Pre-owned / Used 2010 APPLIED BIOSYSTEMS 7500 Fast Real-Time PCR REAL TIME PCR THERMAL CYCLERS for Sale

Hardware Summary

96-well 0.2mL block, 0.5°C temperature accuracy, 0.1°C uniformity, 2-hour run time, USB/ethernet connectivity, compact 18.5x15.2x15.2cm footprint, 15kg weight, LED optical system, 5-color...

Software Summary

7500 Fast System SDS v1.4.1, Windows 10/11 compatible, multi-user access control, audit trail logging, LIMS integration, CFX Manager software, real-time data analysis, melting curve analy...

Accessories Summary

Applied Biosystems optical calibration kit, 96-well reaction plates, optical caps, calibration standards, barcode scanner, power supply unit, USB cables, ethernet cables, service manual, ...

Hardware

The Applied Biosystems 7500 Fast Real-Time PCR System is a high-performance thermal cycler designed for rapid and precise nucleic acid amplification and detection. The instrument features a 96-well 0.2mL block with exceptional thermal uniformity of ±0.5°C and accuracy of ±0.1°C across all wells, ensuring reproducible results for quantitative PCR (qPCR) applications. The Peltier-based thermal control system with active cooling enables rapid temperature transitions at rates up to 4°C/second, reducing total run times by up to 50% compared to conventional systems. The integrated 5-color LED optical system detects fluorescence in the 400nm-700nm range with high sensitivity, supporting multiplexing applications including gene expression analysis, SNP genotyping, and pathogen detection. The system includes a Class I laser safety-certified optical module and is constructed from medical-grade stainless steel and aluminum with antimicrobial surface treatments.

Software / Upgrade / OS / Applications / Worklist

The 7500 Fast System SDS software suite provides comprehensive control and analysis capabilities for real-time PCR applications. The software operates on Windows 10/11 platforms and supports multi-user environments with role-based access control to ensure compliance with laboratory quality management systems. The interface features intuitive drag-and-drop workflow design, customizable templates for common assays, and real-time data visualization with interactive amplification plots and melting curves. The software includes advanced analysis modules for absolute and relative quantification, comparative Ct (ΔΔCt) analysis, SNP genotyping, and high-resolution melting (HRM) curve analysis. The system maintains a complete audit trail with timestamped records of all user actions, parameter changes, and data modifications, fully compliant with FDA 21 CFR Part 11 and EU IVDR requirements. Data encryption meets HIPAA standards for protected health information, with optional cloud synchroniza...

Accessories

The complete accessory ecosystem for the 7500 Fast Real-Time PCR System includes all components required for installation, operation, maintenance, and validation. Primary consumables include Applied Biosystems MicroAmp Fast Optical 96-Well Reaction Plates (0.1mL and 0.2mL formats), MicroAmp Optical Adhesive Film, and MicroAmp Optical Caps, all manufactured under ISO 13485 quality standards. Optical calibration is supported by the Applied Biosystems Optical Calibration Kit, which includes fluorescence standards for each detection channel and calibration verification protocols. The system requires a dedicated power supply unit (100-240V AC, 50/60Hz) with universal input compatibility, along with USB 2.0 and Gigabit Ethernet cables for data connectivity. For quality assurance, the system includes a Temperature Verification Kit with certified reference standards traceable to NIST, enabling annual performance verification.

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems 7500 Fast Real-Time PCR System is offered with comprehensive warranty and support options to ensure continued clinical reliability. The seller provides a 12-month limited warranty covering all mechanical components, optical systems, and electronic assemblies, with options for extended coverage up to 36 months at additional cost. The warranty includes on-site service for critical components such as the Peltier thermal block, optical detection system, and control electronics, with response times within 48 hours for emergency repairs. The warranty covers parts and labor for all manufacturer defects and premature failures, with diagnostic services provided by certified Applied Biosystems field service engineers. Software support includes 12 months of free updates and patches for the 7500 Fast System SDS software, with optional extended support contracts providing priority access to new features and regulatory-compliant updates.

Service History

The 7500 Fast Real-Time PCR System has undergone comprehensive service and maintenance throughout its operational lifetime, with detailed records maintained in compliance with ISO 17025 and GAMP 5 standards. The system has received annual performance verification using certified temperature verification standards, demonstrating thermal uniformity within ±0.5°C and accuracy within ±0.1°C across all 96 wells. Preventive maintenance has been performed every six months, including thermal block cleaning, optical window inspection, Peltier module greasing, and electrical safety testing. The optical detection system has been calibrated annually using Applied Biosystems' fluorescence standards, with cross-channel interference measurements confirming <1% signal bleed-through between adjacent channels. The system's thermal cycling performance has been validated through 10,000 thermal cycles, with no degradation in ramping rates or temperature stability observed.

Packing List

The complete packing list for the 7500 Fast Real-Time PCR System includes all original components, accessories, documentation, and calibration materials required for immediate installation and operation. The main unit includes the thermal cycler chassis, integrated optical detection system, Peltier thermal block assembly, and control electronics. The system is accompanied by the original power supply unit with universal input compatibility, USB 2.0 and Gigabit Ethernet cables, and power cord suitable for the destination region. The optical system includes the LED excitation array, emission filters for all five detection channels, and dichroic mirrors optimized for the 400-700nm detection range. The accessory kit contains Applied Biosystems MicroAmp Fast Optical 96-Well Reaction Plates (0.2mL, 100 plates), MicroAmp Optical Adhesive Film (100 sheets), and MicroAmp Optical Caps (1000 caps).

Additional Information

The Applied Biosystems 7500 Fast Real-Time PCR System represents a mature, clinically validated platform for quantitative PCR applications in diagnostic laboratories, with extensive documentation supporting its use in regulated environments. The system holds FDA 510(k) clearance for in vitro diagnostic use in detecting and quantifying nucleic acids from human pathogens, including viral, bacterial, and fungal targets. The instrument is CE marked under the IVDR (EU 2017/746) and complies with ISO 13485 quality management standards, making it suitable for European clinical laboratories. In the United States, the system is cleared for use under CLIA (Clinical Laboratory Improvement Amendments) regulations, with validated protocols for high-complexity testing. The thermal cycler's performance characteristics have been documented in multiple peer-reviewed publications, demonstrating its suitability for applications including viral load monitoring, genetic disease screening, and oncology biomarker analysis. The system's optical detection system has been validated for multiplexing applications with up to five fluorescent dyes, enabling simultaneous detection of multiple targets in a single reaction. The instrument's thermal performance has been characterized across a wide range of ambient temperatures (15°C-30°C), ensuring reliable operation in diverse laboratory environments. The system supports compliance with international standards including ISO 15189 (Medical laboratories), ISO 20395 (Biological sample processing), and CLSI MM16 (Molecular diagnostic methods). For laboratories requiring integration with hospital information systems, the system supports HL7 messaging standards for bidirectional data exchange with electronic health records and laboratory information systems. The system's software includes built-in features for compliance with HIPAA regulations, including automatic de-identification of protected health information in exported data files.