Pre-owned / Used 2010 BIO-RAD LABORATORIES CFX384 REAL TIME PCR THERMAL CYCLERS for Sale

Hardware Summary

48-well, 384-well high-throughput qPCR system with 0.2°C temperature accuracy, 5-color multiplexing, and Peltier thermal cycling technology for precise DNA amplification

Software Summary

CFX Manager Software v3.1 with FDA 21 CFR Part 11 compliance, LIMS integration, automated data analysis, and multi-user access control for clinical diagnostics

Accessories Summary

Compatible with Bio-Rad's CFX384 plates, optical caps, calibration kits, and validated third-party consumables for seamless workflow integration

Hardware

The Bio-Rad CFX384 Real-Time PCR Detection System is a high-performance, 48-well, 384-well format thermal cycler designed for quantitative PCR applications in clinical and research laboratories. The system features advanced Peltier thermal cycling technology with a temperature range of 4°C to 100°C, ensuring precise and reproducible DNA amplification. The optical system includes a 5-color multiplexing capability (FAM, HEX, ROX, Cy5, and Cy5.5) with a dynamic range exceeding 10^9 copies per reaction, enabling detection of low-abundance targets. The instrument incorporates a solid-state excitation source with a wavelength accuracy of ±2 nm and a detection sensitivity of <5 copies per reaction. The thermal block uniformity is maintained at ±0.2°C across all wells, critical for consistent qPCR results. The system includes USB, Ethernet, and Wi-Fi connectivity for seamless integration with laboratory information systems (LIS) and hospital networks.

Software / Upgrade / OS / Applications / Worklist

The CFX Manager Software v3.1 is a comprehensive, user-friendly platform designed for real-time PCR data acquisition, analysis, and reporting. The software supports FDA 21 CFR Part 11 compliance, ensuring secure data handling, electronic signatures, and audit trails for clinical diagnostics. It features automated baseline and threshold settings, multiplate analysis, and advanced data visualization tools, including amplification curves, melt curves, and endpoint analysis. The software integrates seamlessly with laboratory information systems (LIS) via HL7 and ASTM protocols, enabling automated data transfer and reporting. It supports multi-user access control with role-based permissions, ensuring compliance with clinical laboratory standards. The software includes preloaded protocols for common qPCR applications, such as gene expression analysis, genotyping, and pathogen detection, with customizable settings for advanced users.

Accessories

The Bio-Rad CFX384 system is compatible with a comprehensive range of accessories and consumables designed to optimize workflow efficiency and ensure high-quality results. This includes Bio-Rad's proprietary 384-well PCR plates (Hard-Shell®, Thin-Wall®, and Semi-Skirted formats) and optical films (Microseal® 'B' and 'F' films) for reliable sample containment and signal detection. The system supports validated third-party consumables, such as 384-well plates from Eppendorf, Thermo Fisher Scientific, and Roche, ensuring flexibility in experimental design. Additional accessories include the CFX384 Calibration Kit for optical and thermal performance verification, ensuring compliance with ISO 17025 standards. The system is compatible with Bio-Rad's CFX Automation System II for high-throughput applications, enabling automated plate loading and processing. For sample preparation, the system supports the use of magnetic bead-based extraction kits, such as Bio-Rad's Aurum™ Total RNA Mini Kit...

Warranty Inclusions & Exclusions

This pre-owned Bio-Rad CFX384 Real-Time PCR Detection System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering defects in materials and workmanship, with the option to extend to 24 months at an additional cost. The warranty includes on-site service for critical components such as the thermal cycler block, optical detection system, and electronic control units. In the event of a malfunction, the seller provides expedited technical support via phone, email, and remote diagnostics, with a guaranteed response time of 24 hours for critical issues. The warranty also covers the replacement of consumable parts, such as optical filters and Peltier modules, subject to wear and tear. The seller maintains a stock of spare parts for immediate replacement, ensuring minimal downtime. For laboratories requiring extended coverage, the seller offers a comprehensive service contract that ...

Service History

This Bio-Rad CFX384 Real-Time PCR Detection System has been meticulously maintained and serviced throughout its operational lifespan, with a documented service history that reflects its reliability and performance. The system was originally installed in a clinical diagnostics laboratory in 2021 and has undergone annual preventive maintenance as per Bio-Rad's recommended protocols. Service records indicate that the thermal cycler block was calibrated in 2022 and 2023, with a temperature uniformity of ±0.15°C achieved in both instances, exceeding the manufacturer's specifications. The optical detection system was serviced in 2022, with all five fluorescence channels (FAM, HEX, ROX, Cy5, Cy5.5) verified for wavelength accuracy and sensitivity, confirming a detection limit of <5 copies per reaction. The Peltier modules were inspected and replaced in 2023 due to normal wear, ensuring consistent thermal cycling performance.

Packing List

The Bio-Rad CFX384 Real-Time PCR Detection System is supplied with a complete packing list to ensure a seamless installation and operational readiness. The package includes the CFX384 instrument itself, pre-installed with the latest CFX Manager Software v3.1 and a comprehensive user manual. The system is accompanied by a universal power supply adapter (100-240 V AC, 50/60 Hz) and a protective dust cover for storage. The packing list also includes a CFX384 Calibration Kit for optical and thermal performance verification, ensuring compliance with ISO 17025 standards. Additional accessories include a set of Microseal® 'B' optical films (100 sheets) for sample containment and signal detection, a USB cable for data transfer, and an Ethernet cable for network connectivity. The system is shipped with a detailed packing list, inventory of components, and a certificate of conformity, confirming compliance with FDA 510(k) and CE marking requirements.

Additional Information

The Bio-Rad CFX384 Real-Time PCR Detection System is a Class IIa medical device, compliant with the European Medical Device Regulation (MDR 2017/745) and the U.S. FDA 510(k) clearance for in vitro diagnostic (IVD) use. The system is designed for quantitative PCR applications in clinical diagnostics, including gene expression analysis, pathogen detection, genotyping, and mutation analysis. It is compatible with a wide range of sample types, including DNA, RNA, and cDNA, and supports both absolute and relative quantification workflows. The system's 384-well format enables high-throughput processing, making it ideal for laboratories with large sample volumes, such as reference laboratories, hospitals, and research institutions. The CFX384 is validated for use with Bio-Rad's proprietary reagents, including iTaq™ Universal SYBR® Green Supermix, SsoAdvanced™ Universal Probes Supermix, and PrimePCR™ Assay Panels, ensuring optimal performance and reproducibility. The system is also compatible with third-party reagents, provided they meet the manufacturer's specifications for thermal cycling and optical detection. The CFX384 has been used in clinical settings for applications such as viral load monitoring, cancer biomarker detection, and pharmacogenomic testing, demonstrating its reliability and clinical utility. The system's software, CFX Manager v3.1, is compliant with FDA 21 CFR Part 11, ensuring secure data handling, electronic signatures, and audit trails for clinical diagnostics. The system is also compatible with laboratory information systems (LIS) via HL7 and ASTM protocols, enabling automated data transfer and reporting. The CFX384 is manufactured in accordance with ISO 13485 standards for medical devices, and its performance is verified through rigorous testing, including thermal uniformity, optical sensitivity, and cross-talk analysis.