Pre-owned / Used 2007 APPLIED BIOSYSTEMS 7500 Fast Real-Time PCR THERMAL CYCLERS for Sale

Hardware Summary

96-well block, 0.2mL tubes/strips, ±0.25°C temp accuracy, 512MB RAM, USB 2.0/3.0, Ethernet connectivity, compact 17.8×43.2×38.1cm footprint, 15kg weight, 100-240V power input

Software Summary

7500 Fast System SDS v1.4.1, real-time PCR analysis, multi-user access, audit trails, GLP/GMP compliance, LIMS integration, export formats: CSV, PDF, XML, touchscreen interface

Accessories Summary

Compatible with MicroAmp Fast Optical 96-Well Reaction Plates, optical caps, calibration standards, validation kits, PCR-grade consumables, universal power adapters, Ethernet cables

Hardware

The Applied Biosystems 7500 Fast Real-Time PCR System is a high-performance thermal cycler designed for rapid and precise nucleic acid amplification and detection. The system features a 96-well 0.2mL block with independent Peltier-based thermal control, achieving temperature uniformity of ±0.25°C across all wells. The instrument operates with a temperature range of 4°C to 99°C and a ramp rate of up to 4.5°C/second, enabling ultra-fast cycling protocols. The optical system includes a 50W halogen lamp with six excitation filters (365, 430, 475, 495, 520, 585nm) and five emission filters (520, 555, 585, 620, 665nm), supporting multiplexing capabilities for up to five fluorophores simultaneously. The system is equipped with a 10.4-inch touchscreen display, 512MB RAM, and 4GB internal storage for data processing and storage. Connectivity options include USB 2.0/3.0 ports, Ethernet (10/100), and optional Wi-Fi for seamless integration with laboratory information systems (LIS) and electron...

Software / Upgrade / OS / Applications / Worklist

The 7500 Fast System SDS (Software Development Suite) v1.4.1 is a comprehensive, FDA 21 CFR Part 11-compliant software platform designed for real-time PCR data acquisition, analysis, and reporting. The software supports absolute and relative quantification, melting curve analysis, and endpoint genotyping with advanced algorithms for baseline correction, threshold setting, and Cq determination. Multi-user access control with role-based permissions ensures compliance with GLP/GMP guidelines, while audit trails automatically log all user actions, data modifications, and system events. The software integrates seamlessly with laboratory information management systems (LIMS) via HL7 or ASTM protocols, enabling automated data transfer and reducing manual entry errors. Data export capabilities include CSV, PDF, and XML formats, with support for third-party analysis tools such as Microsoft Excel and GraphPad Prism.

Accessories

The 7500 Fast Real-Time PCR System is compatible with a comprehensive range of accessories and consumables to optimize performance and workflow efficiency. Primary consumables include MicroAmp Fast Optical 96-Well Reaction Plates (0.1mL and 0.2mL formats), MicroAmp Optical Adhesive Film, and MicroAmp Optical 8-Tube Strips with individual caps. Calibration and validation kits include the 7500 Fast Calibration Kit for optical and thermal performance verification, and the 7500 Fast Validation Kit for system qualification. Additional accessories include the 7500 Fast Optical Tube Base for enhanced thermal uniformity, the 7500 Fast Plate Base for improved plate stability, and the 7500 Fast Optical Cover for preventing evaporation during extended runs. The system supports universal power adapters (100-240V AC) with interchangeable plugs for global compatibility, and high-quality Ethernet cables (Cat 5e or Cat 6) for stable network connections.

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems 7500 Fast Real-Time PCR System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering all mechanical, optical, and electronic components, including the Peltier thermal block, optical detection system, and embedded computer. The warranty includes on-site service by certified technicians for critical failures, with a guaranteed response time of 48 hours for in-warranty repairs. Extended warranty options are available for an additional fee, covering up to 36 months of parts and labor, as well as software updates and technical support. The warranty excludes consumables, user-induced damage, and unauthorized modifications. A full refund or replacement option is available within 30 days of delivery, subject to inspection and verification of the system's condition.

Service History

This Applied Biosystems 7500 Fast Real-Time PCR System has been meticulously maintained with a documented service history to ensure optimal performance and reliability. The system has undergone annual preventive maintenance by certified service engineers, including thermal block calibration, optical system alignment, and software validation. The thermal block has been serviced every 6 months to maintain ±0.25°C temperature uniformity, with replacement of worn Peltier elements as needed. The optical system has been cleaned and recalibrated annually, with verification of excitation and emission filter performance using NIST-traceable standards. The embedded computer and touchscreen display have been inspected and updated with the latest firmware revisions to ensure compatibility with current software versions. The system has been subjected to a full performance qualification (PQ) and operational qualification (OQ) protocol, with all results documented and stored in the service history...

Packing List

The Applied Biosystems 7500 Fast Real-Time PCR System is delivered in a custom-fitted, high-density foam-lined shipping crate designed to protect the instrument during transit. The packing list includes the main unit (7500 Fast Real-Time PCR System), power cable (100-240V AC, 1.5m length), Ethernet cable (Cat 6, 3m length), USB cable (2m length), quick start guide, safety instructions, and regulatory compliance documentation. The crate also contains the original accessories shipped with the system, including the MicroAmp Optical 96-Well Reaction Plate base, optical cover, and calibration standards. All components are secured with anti-static foam and labeled for easy identification. The shipping crate is marked with handling instructions, including 'Fragile,' 'This Side Up,' and 'Temperature Controlled' labels to ensure proper handling. The system is packed in accordance with ISTA 3A standards for laboratory equipment, with additional cushioning to absorb shocks during transportatio...

Additional Information

The Applied Biosystems 7500 Fast Real-Time PCR System is a Class II medical device, compliant with FDA 21 CFR Part 820 (Quality System Regulation), EU IVD Directive 98/79/EC, and ISO 13485 standards for medical device manufacturing. The system is registered with the FDA as a Class II exempt device for research use only (RUO) and clinical diagnostics under 510(k) clearance for specific applications, including pathogen detection, genetic testing, and forensic analysis. The system has been validated for use in accordance with the Clinical Laboratory Improvement Amendments (CLIA) and the College of American Pathologists (CAP) guidelines, ensuring compliance with laboratory quality standards. The thermal cycler is compatible with a wide range of PCR chemistries, including TaqMan, SYBR Green, and probe-based assays, with support for both absolute and relative quantification methods. The system's optical detection capabilities enable multiplexing for up to five fluorophores, making it suitable for applications such as pathogen identification, genetic mutation analysis, and gene expression profiling. The device is listed with the Global Medical Device Nomenclature (GMDN) as a 'Thermal Cycler, Polymerase Chain Reaction, Real-Time,' and is classified under GMDN code 37112. The system has been used in accredited clinical laboratories, research institutions, and forensic facilities worldwide, with documented performance in external quality assessment schemes (EQAS) such as the College of American Pathologists (CAP) and the European Molecular Genetics Quality Network (EMQN). The seller provides a certificate of conformity (CE) and a Declaration of Conformity (DoC) for the system, along with a full technical file and risk management documentation in accordance with ISO 14971. The system is also compliant with electromagnetic compatibility (EMC) standards, including IEC 61326-1 and FCC Part 15, ensuring safe operation in clinical and laboratory environments.