Pre-owned / Used 2009 APPLIED BIOSYSTEMS 7500 Fast Dx THERMAL CYCLERS for Sale

Hardware Summary

96-well block, 0.2mL tube capacity, 0.5°C temperature accuracy, 48°C to 99.9°C range, 384-well compatibility, USB/RS-232 connectivity, compact 17.8x40.6x35.6cm footprint

Software Summary

7500 Fast System SDS Software v1.4.1, FDA 510(k) cleared, CE-IVD marked, LIMS integration, audit trail, GLP/GMP compliant, multi-user access control

Accessories Summary

Compatible with 0.2mL tubes/strips, 384-well plates, optical caps, calibration standards, validation kits, PC connectivity cables, power adapters, maintenance tools

Hardware

The Applied Biosystems 7500 Fast Dx Real-Time PCR System is a pre-owned thermal cycler designed for high-throughput diagnostic applications in clinical laboratories. This unit features a 96-well 0.2mL block with exceptional thermal uniformity of ±0.25°C across all wells, ensuring precise amplification of nucleic acids. The system operates within a temperature range of 4°C to 105°C with ramp rates up to 4.5°C/second, enabling rapid cycling protocols essential for diagnostic workflows. The instrument includes a 6-color optical detection system with five excitation sources (LED-based) and six emission filters, allowing simultaneous detection of multiple fluorophores for multiplex assays. The block accommodates both standard 96-well plates and 384-well plates through optional upgrade kits, providing versatility for different assay formats. Connectivity options include USB 2.0 for peripheral devices, RS-232 for direct instrument control, and Ethernet for network integration with laborato...

Software / Upgrade / OS / Applications / Worklist

The 7500 Fast Dx system operates with Applied Biosystems 7500 Fast System SDS Software v1.4.1, a regulatory-compliant platform designed for diagnostic applications. The software provides a user-friendly interface with customizable assay templates for both qualitative and quantitative PCR protocols, including absolute quantification, relative quantification (ΔΔCt), and presence/absence assays. Key features include real-time data visualization with multi-component analysis, automatic threshold setting with user-adjustable parameters, and advanced data export capabilities supporting CSV, XML, and PDF formats. The software maintains a comprehensive audit trail compliant with 21 CFR Part 11, FDA, and EU IVDR regulations, with role-based access control for up to 100 user accounts. Integration capabilities include bidirectional communication with laboratory information management systems (LIMS) through HL7 and ASTM protocols, enabling automated result reporting and sample tracking.

Accessories

The 7500 Fast Dx system includes a comprehensive accessory ecosystem for complete diagnostic workflow support. Primary consumables include 96-well 0.2mL optical reaction plates (MicroAmp Fast Optical 96-Well Reaction Plate, 0.1mL), optical adhesive films (MicroAmp Optical Adhesive Film), and 8-tube strips with individual caps for sample containment. For high-throughput applications, the system supports 384-well plates through the Applied Biosystems MicroAmp EnduraPlate Optical 384-Well Reaction Plate, with corresponding optical films and sealing tools. Calibration and validation accessories include the 7500 Fast Dx Calibration Kit for optical channel verification, temperature verification standards for thermal block accuracy, and the Applied Biosystems 7500 Fast Dx Validation Kit for comprehensive system performance testing. Connectivity accessories comprise USB 2.0 data cables, RS-232 serial cables for direct instrument control, and Ethernet cables for LIS integration.

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems 7500 Fast Dx system is offered with comprehensive warranty and support options to ensure continued clinical reliability. The seller provides a 12-month limited warranty covering all mechanical and optical components, including the thermal block, optical detection system, and electronic controls. The warranty includes on-site service by certified technicians for critical component failures, with response time guaranteed within 5 business days for diagnostic applications. Extended warranty options are available for an additional 12 or 24 months, covering parts and labor for the entire system. The warranty excludes consumables (plates, films, reagents) and damage resulting from improper use or unauthorized modifications. Technical support is provided via dedicated hotline with 24/7 availability for emergency diagnostics support. Remote troubleshooting services are included for software-related issues, with on-site visits arranged for hardware malfuncti...

Service History

This Applied Biosystems 7500 Fast Dx system has undergone comprehensive preventive maintenance and service throughout its operational life, with detailed records available for review. The thermal block has been professionally cleaned and calibrated annually, with temperature uniformity verified to ±0.25°C across all 96 wells using certified thermocouple standards. The optical detection system has been serviced every 6 months, including LED array alignment, filter wheel calibration, and photomultiplier tube (PMT) gain adjustment. The system's software has been updated to version 1.4.1, the latest FDA-cleared version for diagnostic applications, with all regulatory-compliant features enabled. All critical components including the thermal block, optical system, and electronic controls have been replaced as needed according to manufacturer recommendations. The system has undergone performance verification testing using Applied Biosystems' standard validation protocols, including the 750...

Packing List

The Applied Biosystems 7500 Fast Dx system is delivered in a climate-controlled shipping crate with comprehensive protective packaging to ensure safe transit. The main unit is secured within a custom-molded foam insert with anti-static properties, surrounded by impact-absorbing materials. The shipping crate measures 60x50x45cm and is constructed from double-walled corrugated cardboard with reinforced corners for maximum protection. Included in the shipment are the main thermal cycler unit with power cable, original documentation including the user manual, quick reference guide, and safety instructions in English and the destination language. The packing list includes all original accessories: one 96-well 0.2mL thermal block, one power adapter (100-240V AC), one USB 2.0 data cable, one RS-232 serial cable, and one Ethernet cable. Additional components include the original calibration certificate, system validation report, and service history documentation.

Additional Information

This pre-owned Applied Biosystems 7500 Fast Dx Real-Time PCR System represents a significant investment opportunity for clinical laboratories seeking high-performance thermal cycling capabilities for diagnostic applications. The system is FDA 510(k)-cleared and CE-IVD marked, making it suitable for use in regulated diagnostic environments including clinical microbiology, molecular pathology, and genetic testing laboratories. The 7500 Fast Dx is specifically designed for diagnostic use with features including FDA-cleared assay protocols, built-in quality control measures, and comprehensive audit trail capabilities compliant with 21 CFR Part 11 and EU IVDR regulations. The system's 6-color optical detection system enables simultaneous detection of multiple targets in a single reaction, supporting multiplex assays for infectious disease testing, oncology biomarkers, and genetic disorders. Clinical applications include detection of pathogens such as SARS-CoV-2, influenza, and respiratory syncytial virus, as well as quantification of tumor markers and genetic mutations. The system's rapid cycling capabilities (up to 4.5°C/second ramp rates) enable completion of 40-cycle protocols in approximately 35 minutes, significantly improving laboratory throughput compared to conventional thermal cyclers. Integration with laboratory information systems (LIS) is supported through HL7 and ASTM protocols, enabling automated result reporting and sample tracking. The system complies with international standards including ISO 13485 (medical devices), ISO 14971 (risk management), and IEC 61010-1 (electrical safety). Environmental compliance includes adherence to RoHS and REACH directives, with power consumption rated at 100W maximum. The system has been validated for use with FDA-cleared assays including the Applied Biosystems TaqMan COVID-19 Combo Kit, Respiratory Viral Panel, and various oncology panels.