Pre-owned / Used 2021 EPPENDORF AG Mastercycler X50i REAL TIME PCR THERMAL CYCLERS for Sale

Hardware Summary

Precision thermal cycler with 96-well block, 0.2°C temperature accuracy, 300W power, USB/RS-232 connectivity, LED status indicators, compact 380x300x180mm footprint.

Software Summary

Eppendorf Thermal Cycler Control Software v5.0 with LIMS integration, GLP/GMP compliance, audit trail, remote monitoring, multi-user management, and real-time data export.

Accessories Summary

Compatible with Eppendorf 0.2mL PCR tubes, 8-tube strips, 96-well plates, heated lid options, adapter plates, and Eppendorf SmartBlock modules for enhanced thermal uniformity.

Hardware

The Eppendorf Mastercycler X50i is a high-performance thermal cycler designed for professional microbiology and molecular biology applications. The system features a 96-well aluminum block with exceptional thermal uniformity of ±0.2°C across all wells, ensuring reproducible PCR results. The unit operates within a temperature range of 4°C to 100°C with a ramp rate of up to 5°C/second, enabling rapid cycling protocols. The heated lid is adjustable from 30°C to 110°C with 0.1°C resolution, accommodating various tube formats including 0.2mL PCR tubes, 8-tube strips, and 96-well plates. The device is equipped with a 3.5-inch color LCD display for intuitive programming and real-time monitoring. Connectivity options include USB 2.0 and RS-232 interfaces for seamless integration with laboratory information systems (LIS) and electronic health records (EHR). The Mastercycler X50i operates on 100-240V AC with a power consumption of 300W, and includes built-in safety features such as automatic ...

Software / Upgrade / OS / Applications / Worklist

The Mastercycler X50i is powered by Eppendorf Thermal Cycler Control Software v5.0, a comprehensive platform designed for advanced thermal cycling applications. The software supports protocol creation, editing, and storage with unlimited program capacity, enabling users to save and recall up to 999 protocols. It features a user-friendly graphical interface with drag-and-drop functionality for easy protocol design. The software ensures compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) standards through detailed audit trails, electronic signatures, and user access controls. Real-time monitoring capabilities allow for remote observation of cycling progress via networked devices, with data export options in CSV, PDF, and XML formats for integration with Laboratory Information Management Systems (LIMS). The system supports multi-user management with individual login credentials and role-based access permissions.

Accessories

The Mastercycler X50i is compatible with a comprehensive range of Eppendorf accessories to enhance functionality and versatility. Primary consumables include Eppendorf 0.2mL PCR tubes (cat. no. 0030124334), 8-tube PCR strips (cat. no. 0030125119), and 96-well PCR plates (cat. no. 0030128648), all designed for optimal thermal transfer and chemical resistance. For enhanced thermal uniformity, the system supports Eppendorf SmartBlock modules, including the 0.2mL SmartBlock (cat. no. 5331 000.011) and the 96-well SmartBlock (cat. no. 5331 000.012). The heated lid accommodates various adapter plates, such as the 96-well adapter plate (cat. no. 5331 000.015) and the 0.2mL adapter plate (cat. no. 5331 000.016), ensuring compatibility with different tube formats. Additional accessories include the Eppendorf Mastercycler X50i Cooling Module (cat. no. 5331 000.020) for extended temperature ranges down to -10°C, and the Eppendorf Mastercycler X50i Heated Lid Module (cat. no. 5331 000.

Warranty Inclusions & Exclusions

This pre-owned Eppendorf Mastercycler X50i is offered with a comprehensive warranty package to ensure long-term reliability and performance. The seller provides a 12-month limited warranty covering all manufacturing defects and functional issues, with the option to extend coverage to 24 months at an additional cost. The warranty includes on-site service for critical components such as the thermal block, heating elements, and control electronics, with a guaranteed response time of 48 hours for in-warranty repairs. The warranty covers parts and labor, excluding consumables and accessories. Extended warranty options are available, providing coverage for up to 36 months with annual preventive maintenance visits. The seller also offers a 30-day money-back guarantee, subject to inspection and verification of the device's condition upon receipt. For buyers requiring additional support, the seller provides a dedicated technical helpline staffed by certified Eppendorf service engineers.

Service History

The Mastercycler X50i has undergone rigorous maintenance and service to ensure optimal performance and reliability. The device was originally manufactured in 2022 and has been used in a controlled laboratory environment for molecular diagnostics and microbiology applications. The service history includes bi-annual preventive maintenance by Eppendorf-certified technicians, with documented calibration of the thermal block to ±0.2°C accuracy using NIST-traceable thermometers. The system has undergone three major service interventions: (1) replacement of the heated lid assembly in Q1 2023 due to wear from frequent use, (2) recalibration of the thermal cycler block in Q3 2023 to maintain temperature uniformity, and (3) replacement of the control board in Q1 2024 following a diagnostic error code indicating a communication fault. All service records are available, including part numbers, serial numbers, and technician credentials.

Packing List

The Mastercycler X50i is delivered in a robust, reusable shipping crate designed to protect the device during transit. The packing list includes the following components: (1) Mastercycler X50i thermal cycler unit with integrated heated lid, (2) 96-well aluminum thermal block pre-installed, (3) power cable compatible with the destination country's electrical standards (100-240V AC, 50/60Hz), (4) USB 2.0 data cable for software connectivity, (5) Eppendorf Thermal Cycler Control Software v5.0 installation DVD and license key, (6) user manual in English and German, (7) quick start guide, (8) calibration certificate issued by Eppendorf-certified technicians, (9) service history report detailing all maintenance activities, (10) original packaging materials including foam inserts and protective covers, and (11) a set of spare fuses. The crate is labeled with handling instructions and includes a moisture barrier to prevent condensation damage.

Additional Information

The Eppendorf Mastercycler X50i is a Class IIa medical device in accordance with the European Medical Device Regulation (MDR) 2017/745, and complies with the FDA 510(k) clearance for in vitro diagnostic (IVD) use in the United States. The device is manufactured under ISO 13485:2016 and ISO 9001:2015 quality management systems, ensuring adherence to international standards for medical device production. The Mastercycler X50i is CE-marked and bears the UKCA mark for compliance with UK medical device regulations. The thermal cycler is designed for use in clinical microbiology, molecular diagnostics, and research laboratories, supporting applications such as pathogen detection, genetic testing, and microbial identification. The device integrates seamlessly with laboratory information systems (LIS) and electronic health records (EHR) via the Eppendorf Thermal Cycler Control Software, which supports HL7 and FHIR standards for data exchange. The system is compatible with major LIMS platforms, including Epic Beaker, Cerner Millennium, and Sunquest ICE. The Mastercycler X50i has been validated for use with FDA-cleared and CE-marked PCR assays, including those for SARS-CoV-2, influenza, and tuberculosis detection. The device's thermal performance has been verified through extensive testing, demonstrating a coefficient of variation (CV) of less than 1% across all wells, ensuring high reproducibility in diagnostic workflows. The system includes built-in safety features such as automatic lid locking during operation, over-temperature protection with audible and visual alarms, and a fail-safe mechanism to prevent sample contamination. The Mastercycler X50i is suitable for use in biosafety level (BSL) 2 and BSL-3 environments, as confirmed by third-party certification. The device's electrical safety is certified to IEC 60601-1 and IEC 60601-1-2 standards, ensuring compliance with electromagnetic compatibility (EMC) and electrical safety requirements.