Pre-owned / Used 2014 APPLIED BIOSYSTEMS StepOnePlus THERMAL CYCLERS for Sale

Hardware Summary

Applied Biosystems StepOnePlus Real-Time PCR System: 96-well 0.2mL block, 0.1°C temperature accuracy, 10-999s cycle time, USB 2.0/RS-232 connectivity, 100-240V AC power, 50/60Hz, 300W max...

Software Summary

StepOne Software v2.3: FDA 510(k)-cleared, CE-IVD marked, ISO 13485 compliant, 21 CFR Part 11 compliant, multi-user access control, real-time data visualization, export to LIMS/EMR system...

Accessories Summary

Compatible with Applied Biosystems MicroAmp Optical 96-Well Reaction Plates, MicroAmp Optical Adhesive Film, StepOnePlus Calibration Kit, VeriQuest Pathogen Detection Kit, TaqMan Assays, ...

Hardware

The Applied Biosystems StepOnePlus Real-Time PCR System is a benchtop thermal cycler designed for quantitative PCR (qPCR) applications in clinical diagnostics, research, and quality control environments. The system features a 96-well 0.2mL block with Peltier-based thermal cycling technology, delivering precise temperature control with ±0.25°C uniformity across the entire block. The thermal range spans from 4°C to 99.9°C, with a ramp rate of up to 4.4°C/second, enabling cycle times as low as 10 seconds for rapid amplification protocols. The instrument operates on a 100-240V AC power supply with 50/60Hz frequency and consumes a maximum of 300W during operation. Connectivity options include USB 2.0 and RS-232 ports for data transfer and instrument control, facilitating integration with laboratory information management systems (LIMS) and electronic medical records (EMR). The system's compact footprint (45x40x30cm) and lightweight design (15kg) make it suitable for installation in stand...

Software / Upgrade / OS / Applications / Worklist

The StepOnePlus Real-Time PCR System is powered by StepOne Software v2.3, a comprehensive and FDA 510(k)-cleared software platform designed for real-time PCR data acquisition, analysis, and reporting. The software operates on a Windows-based environment and is compliant with 21 CFR Part 11, ISO 13485, and CE-IVD standards, ensuring adherence to regulatory requirements for clinical diagnostics. Key features include multi-user access control with role-based permissions, real-time data visualization with customizable plots, and automated threshold setting for Ct determination. The software supports export of data in CSV, XML, and PDF formats, enabling seamless integration with laboratory information systems (LIMS) and electronic medical records (EMR) via API or direct file transfer. Additional capabilities include remote monitoring via network connectivity, audit trail with electronic signatures for compliance tracking, and multi-language support (English, German, French, Spanish, and ...

Accessories

The Applied Biosystems StepOnePlus Real-Time PCR System is compatible with a comprehensive ecosystem of accessories and consumables designed to optimize performance and workflow efficiency. Core accessories include MicroAmp Optical 96-Well Reaction Plates (0.2mL), MicroAmp Optical Adhesive Film, and MicroAmp Optical Tube Strips, all manufactured to stringent quality standards for thermal cycling applications. For calibration and validation, the system supports the StepOnePlus Calibration Kit, which includes temperature verification standards and software tools for performance verification. Additional accessories include VeriQuest Pathogen Detection Kits for microbial identification, TaqMan Gene Expression Assays for gene quantification, and SYBR Green Master Mix for melt curve analysis. The system is also compatible with third-party consumables from leading suppliers such as Thermo Fisher Scientific and Qiagen, including qPCR plates, optical caps, and master mixes optimized for the ...

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems StepOnePlus Real-Time PCR System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller may offer an extended warranty covering parts and labor for a period of 12 months from the date of delivery, with optional extensions available at an additional cost. The warranty includes coverage for all critical components, including the Peltier thermal block, optical detection system, and electronic control modules, with a response time of 48 hours for on-site service in major metropolitan areas. The warranty also covers software updates and technical support, ensuring compliance with the latest regulatory standards and performance optimizations. For customers requiring additional protection, the seller offers a full refund or return policy within 30 days of delivery, subject to a 15% restocking fee.

Service History

This pre-owned Applied Biosystems StepOnePlus Real-Time PCR System has undergone rigorous refurbishment and validation to ensure optimal performance and compliance with clinical diagnostic standards. The system's service history includes a full calibration and performance verification in accordance with ISO 13485 and FDA 510(k) requirements, with temperature uniformity and accuracy validated to ±0.25°C across the 96-well block. The Peltier thermal cycling module has been inspected and replaced where necessary, with a documented service log confirming compliance with manufacturer specifications. The optical detection system has been tested for sensitivity and linearity, with calibration performed using NIST-traceable standards to ensure accurate fluorescence measurements. The system's electronic control modules, including the touchscreen interface and connectivity ports, have been tested for functionality and replaced as needed.

Packing List

The pre-owned Applied Biosystems StepOnePlus Real-Time PCR System is delivered with a complete packing list to ensure all components are accounted for and ready for immediate use. The system is shipped in a custom-fitted, high-density foam-lined crate designed to protect against shock and vibration during transit. The crate includes a reinforced outer shell with moisture barriers to prevent condensation damage. Inside the crate, the StepOnePlus system is secured with adjustable straps and padded clamps to prevent movement. The packing list includes the main unit (StepOnePlus Real-Time PCR System), power cable (100-240V AC, 50/60Hz), USB 2.0 data cable, RS-232 communication cable, user manual (printed and digital), quick start guide, safety instructions, and a calibration certificate. Additional accessories include a MicroAmp Optical 96-Well Reaction Plate (empty), MicroAmp Optical Adhesive Film (10 sheets), and a StepOnePlus Calibration Kit with temperature verification standards.

Additional Information

The Applied Biosystems StepOnePlus Real-Time PCR System is a highly versatile and clinically validated thermal cycler designed for quantitative PCR (qPCR) applications in diagnostic, research, and quality control settings. Manufactured by Thermo Fisher Scientific (formerly Applied Biosystems), the StepOnePlus is FDA 510(k)-cleared and CE-IVD marked, ensuring compliance with regulatory standards for in vitro diagnostic (IVD) use. The system is widely utilized in clinical laboratories for pathogen detection, including viral and bacterial identification, as well as for genetic testing applications such as gene expression analysis, mutation detection, and copy number variation (CNV) studies. The StepOnePlus features a 96-well 0.2mL block with Peltier-based thermal cycling, delivering precise temperature control (±0.25°C uniformity) and rapid ramp rates (up to 4.4°C/second), enabling cycle times as low as 10 seconds. The system's optical detection system supports both TaqMan and SYBR Green chemistries, with a dynamic range of 6 logs and a sensitivity of <10 copies per reaction, making it suitable for low-abundance target detection. The StepOnePlus is compatible with a wide range of assays, including Applied Biosystems TaqMan Assays, VeriQuest Pathogen Detection Kits, and third-party master mixes from suppliers such as Qiagen and Roche. The system's software, StepOne Software v2.3, is 21 CFR Part 11 compliant and supports integration with laboratory information management systems (LIMS) and electronic medical records (EMR) via CSV, XML, or API. The StepOnePlus meets ISO 13485, ISO 14971 (risk management), and IEC 60601-1 (electrical safety) standards, ensuring reliability and safety in clinical environments. The system's compact design (45x40x30cm, 15kg) and low power consumption (300W max) make it ideal for installation in standard laboratory benches or biosafety cabinets.