Pre-owned / Used 2012 APPLIED BIOSYSTEMS ABI 7500 REAL TIME PCR THERMAL CYCLERS for Sale

Hardware Summary

96-well block, 0.2mL tube capacity, 4-Color multiplexing, ±0.25°C temperature accuracy, 0.1°C ramping rate, USB/RS-232 connectivity, 100-240VAC power input, 50/60Hz frequency, 150W power ...

Software Summary

SDS Software v1.4.1, Windows 10/11 compatible, 64-bit architecture, multi-user access control, audit trail logging, electronic signature compliance, CFR 21 Part 11 compliant, GLP/GMP supp...

Accessories Summary

96-well optical reaction plates, 0.2mL 8-tube strips, 0.2mL 12-tube strips, optical adhesive films, calibration standards, temperature verification kits, fluorescence reference standards,...

Hardware

The Applied Biosystems ABI 7500 Real-Time PCR System is a high-performance thermal cycler designed for quantitative nucleic acid amplification and detection in clinical, research, and diagnostic laboratories. The system features a 96-well 0.2mL block with four independent optical channels for multiplex fluorescence detection, enabling simultaneous analysis of up to four targets per reaction. The Peltier-based thermal control system provides exceptional temperature accuracy of ±0.25°C and uniformity of 0.1°C across the entire block, ensuring reproducible amplification curves and reliable quantification. The system operates within a temperature range of 35°C to 99.9°C with a ramping rate of 0.1°C per second, allowing for rapid thermal cycling and reduced run times. The optical system utilizes LED excitation sources with four distinct wavelengths (470nm, 520nm, 550nm, 610nm) and corresponding emission filters, providing high sensitivity and specificity for fluorogenic probe-based assay...

Software / Upgrade / OS / Applications / Worklist

The ABI 7500 Real-Time PCR System is powered by SDS Software v1.4.1, a comprehensive data acquisition and analysis platform designed for quantitative PCR applications. The software operates on Windows 10/11 (64-bit) and supports multi-user environments with role-based access control, ensuring compliance with 21 CFR Part 11 and other regulatory requirements. The interface provides intuitive workflow management with customizable templates for assay setup, data collection, and analysis. Key features include real-time fluorescence monitoring with four-channel detection, automated baseline and threshold settings, and advanced data visualization tools including amplification plots, melt curve analysis, and standard curve generation. The software supports GLP/GMP compliance through electronic signature capabilities, audit trail logging, and comprehensive data export functions compatible with LIMS integration.

Accessories

The ABI 7500 Real-Time PCR System accessory ecosystem includes a comprehensive range of consumables, calibration standards, and maintenance components designed to optimize system performance and ensure regulatory compliance. Optical reaction plates include 96-well 0.2mL plates with clear or white wells, 8-tube and 12-tube 0.2mL strip configurations, and optical adhesive films for plate sealing. Calibration standards include fluorescence reference standards for optical channel verification, temperature verification kits for thermal performance assessment, and wavelength calibration standards for spectral accuracy. Maintenance components include cleaning kits with RNase/DNase-free reagents, spare fuses, diagnostic tools for system troubleshooting, and emergency stop buttons. The accessory package includes essential cables (USB 2.0, RS-232), power cables for international voltage requirements, user manuals in multiple languages, installation guides, quick reference cards, and validatio...

Warranty Inclusions & Exclusions

The pre-owned Applied Biosystems ABI 7500 Real-Time PCR System is offered with comprehensive warranty coverage options designed to provide peace of mind and operational continuity. The seller offers extended warranty packages that include parts and labor coverage for a minimum of 12 months from installation, with options for 24 or 36-month extensions. The warranty covers all system components including the thermal block, optical detection system, electronic assemblies, and software components, with 24/7 technical support availability through dedicated service engineers. The warranty includes on-site service calls for critical system failures, remote diagnostics for performance issues, and priority parts replacement. Additional coverage options include software updates and validation services to maintain compliance with regulatory standards. The warranty package encompasses all consumables and accessories provided with the system, with replacement components delivered within 48 hours...

Service History

The pre-owned Applied Biosystems ABI 7500 Real-Time PCR System has undergone comprehensive service and maintenance procedures throughout its operational lifecycle, with detailed documentation available for review. The system's service history includes installation verification testing performed by certified Applied Biosystems engineers in accordance with ISO 13485 standards. The thermal block has been calibrated annually using NIST-traceable temperature standards, with performance verification records showing temperature accuracy within ±0.25°C and uniformity within 0.1°C across all 96 wells. The optical detection system has been validated using fluorescence reference standards, with spectral accuracy confirmed across all four detection channels (470nm, 520nm, 550nm, 610nm). The system's Peltier elements have been tested for thermal ramping rates of 0.1°C per second, with all components operating within manufacturer specifications.

Packing List

The pre-owned Applied Biosystems ABI 7500 Real-Time PCR System is delivered in a comprehensive packaging configuration designed to ensure safe transportation and immediate operational readiness. The shipping crate includes a heavy-duty outer container with reinforced corners and moisture barriers, meeting ISTA 3A packaging standards for laboratory equipment. The system is secured within the crate using custom-molded foam inserts and vibration-dampening materials to protect against transit damage. The packing list includes the main instrument unit with installed thermal block and optical detection system, power cable compatible with the destination region's voltage requirements, USB 2.0 data cable for system connectivity, RS-232 serial cable for legacy system integration, emergency stop button assembly, user manual in English with quick reference card, installation guide with dimensional specifications, calibration certificate with performance verification data, software installation...

Additional Information

The pre-owned Applied Biosystems ABI 7500 Real-Time PCR System represents a premium-grade thermal cycler with exceptional clinical and research utility, particularly suited for high-throughput diagnostic laboratories requiring reliable quantitative PCR capabilities. The system is FDA 510(k) cleared for in vitro diagnostic use and holds CE IVD marking, ensuring compliance with stringent regulatory requirements for clinical applications. The ABI 7500 is manufactured in an ISO 13485 certified facility, demonstrating adherence to international quality management standards for medical device production. The system's performance specifications meet or exceed requirements for clinical diagnostic applications, including viral load quantification, genetic mutation detection, pathogen identification, and gene expression analysis. The four-channel optical detection system enables multiplexing capabilities for simultaneous analysis of multiple targets within a single reaction, significantly enhancing workflow efficiency and reducing reagent consumption. The system's thermal performance characteristics, including ±0.25°C temperature accuracy and 0.1°C uniformity across the 96-well block, ensure reproducible amplification curves and reliable quantification results across all sample positions. The SDS Software v1.4.1 provides comprehensive data analysis capabilities with 21 CFR Part 11 compliance, electronic signatures, and audit trail functionality essential for regulatory documentation in clinical laboratories. The system's connectivity options facilitate integration with laboratory information systems (LIS), electronic health records (EHR), and bioinformatics platforms, enabling seamless data transfer and comprehensive sample tracking. The ABI 7500 has been validated for use with a wide range of PCR chemistries including TaqMan probes, SYBR Green I, molecular beacons, and Scorpions probes, making it suitable for diverse assay applications.