Pre-owned / Used 2013 APPLIED BIOSYSTEMS StepOnePlus THERMAL CYCLERS for Sale

Hardware Summary

96-well thermal cycler, 0.2°C temperature accuracy, 0.5°C uniformity, 25-99°C range, USB/RS-232 connectivity, compact benchtop design, LED display, real-time data monitoring

Software Summary

StepOnePlus Software v2.3, FDA 510(k) cleared, CE IVD marked, ISO 13485 compliant, LIMS integration, data export to CSV/Excel, multi-user access control, audit trail logging

Accessories Summary

Compatible with 0.2mL 8-tube strips, 96-well plates, optical caps, calibration verification kits, validation tools, and Applied Biosystems accessories

Hardware

The Applied Biosystems StepOnePlus Real-Time PCR System is a benchtop thermal cycler designed for quantitative PCR (qPCR) applications in clinical and research laboratories. The system features a 96-well block with a temperature range of 25°C to 99°C, achieving a temperature accuracy of ±0.2°C and uniformity of ±0.5°C across the block. The aluminum block ensures rapid thermal transfer and consistent sample processing. Connectivity options include USB 2.0 and RS-232 interfaces for seamless integration with laboratory information management systems (LIMS) and data analysis software. The system is equipped with a high-resolution LED display for real-time monitoring of amplification curves and cycle thresholds (Ct). The compact footprint (43.2 cm W x 48.3 cm D x 38.1 cm H) and lightweight design (22.7 kg) make it ideal for space-constrained laboratory environments. The thermal cycler is constructed from medical-grade stainless steel and high-impact ABS plastic, ensuring durability and c...

Software / Upgrade / OS / Applications / Worklist

The StepOnePlus Real-Time PCR System is powered by the StepOnePlus Software v2.3, a comprehensive and user-friendly platform designed for qPCR data acquisition, analysis, and reporting. The software supports multi-user access with role-based permissions, ensuring compliance with 21 CFR Part 11 requirements for electronic records and signatures. Data export capabilities include CSV, Excel, and PDF formats, enabling seamless integration with laboratory information systems (LIS) and electronic health records (EHR). The software features advanced analysis tools, including baseline correction, threshold settings, and multi-component analysis for multiplex PCR assays. It supports absolute and relative quantification methods, as well as melt curve analysis for genotyping and mutation detection. The system is compatible with Applied Biosystems' TaqMan and SYBR Green assays, as well as third-party chemistries.

Accessories

The StepOnePlus thermal cycler is compatible with a wide range of accessories designed to enhance functionality and ensure compliance with clinical standards. Included accessories comprise 0.2mL 8-tube strips (125 strips per pack), 96-well optical reaction plates (50 plates per pack), and optical caps (1000 caps per pack) for secure sample containment and fluorescence detection. Additional recommended accessories include the Applied Biosystems Calibration Verification Kit for routine performance validation, the VeriFlex Block for enhanced thermal uniformity, and the StepOnePlus Validation Tool for comprehensive system testing. The system supports third-party accessories such as Eppendorf twin.tec PCR plates, Thermo Fisher Scientific MicroAmp Optical 96-Well Reaction Plates, and Qiagen Rotor-Gene Q accessories. For data management, the system is compatible with Applied Biosystems' QuantStudio Design & Analysis Software for advanced data analysis and reporting.

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems StepOnePlus Real-Time PCR System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering all manufacturing defects and functional issues, including thermal block performance, electronic components, and software malfunctions. The warranty includes on-site service by certified Applied Biosystems technicians, ensuring minimal downtime and compliance with manufacturer specifications. Extended warranty options are available at an additional cost, including 24-month and 36-month coverage with priority technical support and annual performance validation. The warranty is transferable to new owners, provided the system remains in its original configuration and is serviced exclusively by authorized personnel. Technical support is available 24/7 via phone, email, and live chat, with a guaranteed response time of 4 business hours for critical issues.

Service History

The StepOnePlus Real-Time PCR System has undergone rigorous maintenance and service history, ensuring optimal performance and compliance with clinical standards. The system was last serviced on [Date], with a comprehensive performance validation conducted by certified Applied Biosystems technicians. The service included calibration of the thermal block to ensure ±0.2°C accuracy and ±0.5°C uniformity, verification of the optical detection system, and testing of all electronic components. The system's firmware and software were updated to the latest version (v2.3), and a full diagnostic test was performed to confirm compliance with manufacturer specifications. Prior to this, the system underwent annual preventive maintenance, including cleaning of the thermal block, inspection of electrical connections, and calibration verification using Applied Biosystems' Calibration Verification Kit. The system has been used in a controlled laboratory environment with consistent environmental condi...

Packing List

The StepOnePlus Real-Time PCR System is delivered in a secure, double-walled cardboard shipping crate with internal foam padding to protect against shock and vibration during transit. The crate measures 60 cm (L) x 60 cm (W) x 70 cm (H) and is labeled with handling instructions and hazard warnings. The system is secured to a wooden pallet for stability, with additional protective covers for the thermal block and optical components. The packing list includes the thermal cycler unit, power cable (100-240V AC, 50/60Hz), USB 2.0 data cable, RS-232 interface cable, user manual (printed and digital), quick start guide, and a USB flash drive containing the StepOnePlus Software v2.3 installer and documentation. Additional accessories included in the shipment are a set of 0.2mL 8-tube strips (50 strips), a 96-well optical reaction plate (10 plates), and a pack of optical caps (200 caps). The shipment is accompanied by a certificate of conformity, calibration certificate, and a packing list w...

Additional Information

The Applied Biosystems StepOnePlus Real-Time PCR System is a Class II medical device certified under the FDA 510(k) pathway (K103147) and CE marked under the In Vitro Diagnostic Directive (IVDD) 98/79/EC, ensuring compliance with stringent regulatory requirements for clinical use. The system is designed for quantitative PCR (qPCR) applications, including pathogen detection, gene expression analysis, genotyping, and mutation detection, making it suitable for clinical diagnostics, research laboratories, and public health facilities. The StepOnePlus system supports a wide range of assay chemistries, including TaqMan, SYBR Green, and probe-based assays, with compatibility for both singleplex and multiplex reactions. The system's thermal block accommodates 96-well plates, 8-tube strips, and 0.2mL tubes, providing flexibility for various experimental setups. The optical detection system utilizes a 488 nm LED excitation source and a CCD camera for fluorescence detection, enabling high sensitivity and specificity in target amplification. The system is validated for use with Applied Biosystems' proprietary assays, as well as third-party chemistries, ensuring broad compatibility with existing laboratory workflows. The StepOnePlus Software v2.3 is 21 CFR Part 11 compliant, featuring secure user authentication, audit trail logging, and electronic signature capabilities for regulatory compliance. The system is compatible with laboratory information management systems (LIMS) and electronic health records (EHR) via CSV, Excel, and PDF data export formats. The thermal cycler is manufactured in compliance with ISO 13485 and ISO 14971 standards, ensuring adherence to quality management and risk management principles. The system's compact design and low power consumption (150W) make it ideal for deployment in resource-constrained environments, including point-of-care testing and field laboratories.