Pre-owned / Used 2011 BIO-RAD LABORATORIES CFX96 REAL TIME PCR THERMAL CYCLERS for Sale

Hardware Summary

6-channel real-time PCR with 96-well block, 0.2°C temperature accuracy, 5-384 sample capacity, USB/ETH/LAN connectivity, compact 17.8x17.8x17.8cm footprint

Software Summary

CFX Manager Software v3.1 with FDA 21 CFR Part 11 compliance, LIMS integration, multi-user access control, automated data analysis with Ct calling algorithms

Accessories Summary

Compatible with all Bio-Rad PCR plates/caps, includes calibration kits, validation standards, and consumables starter pack with certified RNase/DNase-free tubes

Hardware

The Bio-Rad CFX96 Real-Time PCR Detection System is a high-performance thermal cycler designed for quantitative PCR applications in clinical diagnostics. The instrument features a 96-well 0.2 mL block with independent Peltier temperature control for each well, achieving ±0.2°C temperature uniformity across the block. The system supports a temperature range of 4-100°C with ramp rates up to 5°C/second, enabling rapid cycling protocols. Optical system includes a 50 mW 488 nm solid-state laser excitation source with four-color detection channels (FAM/SYBR Green, HEX/VIC, ROX/Texas Red, Cy5). Connectivity options include Gigabit Ethernet, USB 2.0, and optional Wi-Fi for seamless integration with laboratory information systems. The unit incorporates active cooling with a thermal management system that maintains consistent performance during extended runs. External dimensions are 17.8 x 17.8 x 17.8 cm with a weight of 4.5 kg. Power requirements are 100-240 VAC, 50/60 Hz, 2.0 A maximum.

Software / Upgrade / OS / Applications / Worklist

The CFX Manager Software v3.1 is a comprehensive platform for real-time PCR data acquisition and analysis, designed with clinical laboratory workflows in mind. The software operates on Windows 10/11 (64-bit) and provides FDA 21 CFR Part 11 compliant features including user authentication, audit trails, and electronic signatures. Key capabilities include automated baseline and threshold settings, multiplate analysis with inter-run comparison tools, and advanced data visualization with export options to PDF, Excel, and LIMS systems. The software supports absolute and relative quantification methods, including standard curve analysis and comparative Ct (ΔΔCt) calculations. Integration features include compatibility with HL7 and ASTM standards for seamless data transfer to hospital information systems. The platform includes pre-configured protocols for common assays and supports custom assay design with melting curve analysis.

Accessories

The CFX96 system is compatible with a comprehensive range of Bio-Rad accessories and consumables designed to optimize PCR workflows. This includes 96-well PCR plates (low profile, semi-skirted, skirted) with optical-quality sealing films and caps, available in clear and white formats for different detection requirements. The system supports 384-well plates through optional conversion kits. Certified consumables include RNase/DNase-free 0.2 mL tubes and strips, as well as validated master mixes such as iTaq Universal Probes Supermix and SsoAdvanced Universal SYBR Green Supermix. Additional accessories include the CFX96 calibration kit for optical and thermal verification, validation standards for temperature uniformity testing, and the CFX96 validation plate for performance verification. The system is compatible with third-party plates and reagents that meet Bio-Rad's specifications. All consumables carry CE-IVD marking where applicable and are manufactured under ISO 13485 certified ...

Warranty Inclusions & Exclusions

This pre-owned Bio-Rad CFX96 Real-Time PCR Detection System is offered with comprehensive warranty coverage options to ensure clinical reliability. The seller provides a 12-month limited warranty covering all manufacturing defects and component failures, with optional extended warranty packages available at additional cost. The warranty includes replacement of defective components, on-site service for critical failures, and priority access to Bio-Rad's technical support team. Service coverage encompasses the thermal block assembly, optical detection system, and electronic components, with response times within 48 business hours for urgent issues. The warranty is transferable to new owners and includes coverage for software updates and regulatory compliance maintenance. Additional service contracts are available for extended coverage periods (24-36 months) with options for preventative maintenance visits, calibration services, and performance verification testing.

Service History

This Bio-Rad CFX96 system has undergone comprehensive service and maintenance history verification to ensure optimal performance. The unit has received regular preventative maintenance every 6 months, including thermal block calibration, optical system verification, and electrical safety testing. All service records are documented in Bio-Rad's service management system, with the most recent maintenance performed 3 months prior to listing. The instrument has never experienced critical component failures and maintains full compliance with original manufacturer specifications. Calibration certificates are available for temperature uniformity (±0.2°C), ramp rates (5°C/sec), and optical sensitivity across all four detection channels. The system has been used in a controlled laboratory environment with consistent environmental conditions (20-25°C, 40-60% humidity). No liquid spills or mechanical damage have been reported, and all consumables have been stored according to manufacturer guid...

Packing List

The complete packing list for this Bio-Rad CFX96 Real-Time PCR Detection System includes the main instrument unit with integrated computer and display, power cable (100-240V), USB cable for data transfer, Ethernet cable for network connectivity, and comprehensive documentation including the operator's manual, service manual, and quick start guide. The package contains the original Bio-Rad calibration kit with verification standards, a set of certified 96-well PCR plates (low profile, clear), optical sealing films, and RNase/DNase-free 0.2 mL tubes. Additional accessories include the CFX Manager Software installation media (USB drive), license key documentation, and a starter pack of consumables including master mix and primers. The shipment is secured in a custom-molded foam insert within a durable corrugated shipping container, with dimensions of 60x50x50 cm and total weight of 12 kg. All components are individually wrapped and protected against electrostatic discharge.

Additional Information

This Bio-Rad CFX96 Real-Time PCR Detection System represents a premium solution for clinical diagnostic laboratories requiring high-performance quantitative PCR capabilities. The instrument is manufactured by Bio-Rad Laboratories, a global leader in life science research and clinical diagnostics with ISO 13485 certification for medical device quality management. The CFX96 system is FDA 510(k) cleared for in vitro diagnostic use in the United States and carries the CE mark for compliance with European IVD Directive 98/79/EC. The instrument meets all requirements of ISO 15189 for medical laboratories and is compatible with major hospital information systems through HL7 integration protocols. Clinical applications include infectious disease testing, genetic screening, oncology biomarker analysis, and pharmacogenomic studies. The system supports all major real-time PCR chemistries including TaqMan probes, SYBR Green, and molecular beacons. Performance specifications include a dynamic range of 7 logs with detection sensitivity down to 10 copies of target DNA. The instrument features an intuitive touchscreen interface with customizable protocols and automated data analysis. Regulatory compliance includes adherence to IEC 61010-1 safety standards, IEC 61326-1 electromagnetic compatibility requirements, and ISO 14971 risk management standards. The system has been validated for use with clinical samples including blood, serum, plasma, and tissue extracts. Bio-Rad provides comprehensive technical support through a global network of certified service engineers, with 24/7 emergency support available for critical applications. The unit is suitable for deployment in biosafety level 2 laboratories and meets all requirements for clinical laboratory accreditation by organizations such as CAP and CLIA. All documentation is available in multiple languages and includes detailed validation protocols for assay development and verification.