Pre-owned / Used 2008 APPLIED BIOSYSTEMS 7500 FAST THERMAL CYCLERS for Sale

Hardware Summary

96-well block, 0.2ml tubes, 1°C/sec ramp rate, Peltier thermal system, USB/RS232 connectivity, compact 15.5x18.5x10.5in footprint, 25kg weight, 100-240V AC power

Software Summary

7500 Fast System SDS v1.4.1, real-time fluorescence detection, 4-color multiplexing, data export to LIMS, FDA 21 CFR Part 11 compliant, audit trail, user authentication

Accessories Summary

Compatible with MicroAmp Fast Optical 96-Well Reaction Plates, optical caps, calibration standards, validation kits, PC connectivity cables, power adapters

Hardware

The Applied Biosystems 7500 FAST Real-Time PCR System is a high-performance thermal cycler designed for rapid and precise DNA amplification and quantification. This pre-owned unit features a 96-well 0.2ml block with a Peltier-based thermal system capable of achieving ramp rates up to 1°C per second, ensuring efficient thermal cycling for applications such as quantitative PCR (qPCR), gene expression analysis, and pathogen detection. The system operates on a 100-240V AC power supply with a compact footprint of 15.5 x 18.5 x 10.5 inches and weighs 25kg, making it suitable for laboratory bench-top deployment. Connectivity options include USB and RS232 ports for seamless integration with laboratory information management systems (LIMS) and data analysis software. The thermal block accommodates standard 0.2ml MicroAmp Fast Optical 96-Well Reaction Plates, enabling high-throughput processing with minimal sample volume requirements.

Software / Upgrade / OS / Applications / Worklist

The 7500 FAST system is powered by the 7500 Fast System SDS (Software Development Suite) v1.4.1, a comprehensive platform for real-time PCR data acquisition, analysis, and reporting. The software supports 4-color multiplexing, enabling simultaneous detection of multiple targets within a single reaction. Key features include real-time fluorescence monitoring, absolute and relative quantification, melting curve analysis, and comparative Ct (ΔΔCt) analysis for gene expression studies. The software is fully compliant with FDA 21 CFR Part 11 regulations, providing secure user authentication, audit trails, and electronic signatures for regulatory compliance in clinical settings. Data can be exported in multiple formats (CSV, XML, PDF) for integration with laboratory information systems (LIMS) and electronic health records (EHR). The user interface is designed for intuitive operation, with customizable assay templates and automated data analysis workflows to streamline laboratory processes...

Accessories

The 7500 FAST thermal cycler is compatible with a comprehensive range of accessories to optimize performance and workflow efficiency. Essential consumables include MicroAmp Fast Optical 96-Well Reaction Plates (0.1ml and 0.2ml formats), MicroAmp Optical Adhesive Film, and MicroAmp Optical Caps for sealing reactions. Calibration and validation kits, such as the 7500 Fast System Calibration Kit and the 7500 Fast System Validation Kit, ensure thermal and optical performance within specified tolerances. Additional accessories include PC connectivity cables (USB and RS232), power adapters for international voltage standards, and replacement thermal blocks for expanded applications. The system is also compatible with Applied Biosystems' proprietary reagents, including TaqMan Gene Expression Assays, TaqMan MicroRNA Assays, and TaqMan Pathogen Detection Kits, enabling seamless integration with established molecular diagnostics workflows.

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems 7500 FAST Real-Time PCR System is offered with a comprehensive warranty package to ensure operational reliability and peace of mind. The seller provides a 12-month limited warranty covering defects in materials and workmanship, subject to standard terms and conditions. The warranty includes on-site service for critical components such as the thermal block, Peltier elements, and optical detection system, with response times within 48 business hours for urgent repairs. Extended warranty options are available at an additional cost, including 24-month and 36-month coverage with options for parts and labor reimbursement. The warranty also encompasses software updates and technical support for the 7500 Fast System SDS software, ensuring compliance with the latest regulatory standards. Service agreements can be customized to include preventive maintenance, calibration checks, and performance validation, with options for remote diagnostics and troubleshooti...

Service History

This Applied Biosystems 7500 FAST Real-Time PCR System has undergone rigorous service and maintenance to ensure optimal performance and reliability. The service history includes annual preventive maintenance by certified technicians, with detailed records of thermal block calibration, optical system alignment, and electrical safety inspections. The system has been serviced in accordance with Applied Biosystems' recommended protocols, including replacement of wear components such as thermal block seals and optical filters. Performance validation records confirm compliance with manufacturer specifications for thermal uniformity (±0.25°C across all wells) and fluorescence detection sensitivity (signal-to-noise ratio > 500:1). The system has been used in a controlled laboratory environment with documented adherence to ISO 17025 standards for calibration and testing. No major component failures or unscheduled downtime have been recorded, and the system has been regularly updated with the...

Packing List

The Applied Biosystems 7500 FAST Real-Time PCR System is supplied with a complete packing list to ensure a seamless installation and operational readiness. The package includes the thermal cycler unit with integrated computer and monitor, a detachable keyboard and mouse, and a power cord compatible with the destination region's electrical standards. Accessories provided are a MicroAmp Fast Optical 96-Well Reaction Plate (0.2ml, 10 units), MicroAmp Optical Adhesive Film (50 sheets), and MicroAmp Optical Caps (100 units). Documentation includes the 7500 Fast System User Guide, Safety Instructions, and Quick Start Guide, along with a USB drive containing the 7500 Fast System SDS software and drivers. Additional items include a calibration certificate, performance validation report, and a service history log. The system is shipped in a custom-fitted, shock-resistant crate with internal cushioning to prevent transit damage.

Additional Information

The Applied Biosystems 7500 FAST Real-Time PCR System is a Class II medical device designed for in vitro diagnostic (IVD) use, compliant with FDA 510(k) clearance and CE Marking under the In Vitro Diagnostic Directive (IVDD) 98/79/EC. The system is manufactured in accordance with ISO 13485:2016 quality management standards and adheres to IEC 60601-1-2 electromagnetic compatibility (EMC) requirements. Clinical applications include pathogen detection (e.g., SARS-CoV-2, influenza, HIV), gene expression analysis, SNP genotyping, and microbial identification in clinical microbiology laboratories. The system's high ramp rate and thermal precision make it ideal for rapid cycling protocols, reducing turnaround times for critical diagnostic tests. Integration capabilities include compatibility with laboratory information systems (LIS) via HL7 or ASTM standards, enabling automated result reporting and electronic health record (EHR) interfacing. The system supports a wide range of reagents, including Applied Biosystems' TaqMan and SYBR Green assays, as well as third-party chemistries, ensuring flexibility for diverse molecular diagnostics workflows. Safety features include automatic lid heating to prevent condensation, over-temperature protection, and compliance with IEC 62304 software lifecycle standards. The system is suitable for deployment in biosafety level 2 (BSL-2) environments and meets the requirements of the Clinical Laboratory Improvement Amendments (CLIA) for moderate to high-complexity testing. Environmental specifications include operating temperatures between 15°C and 30°C and relative humidity of 20% to 80%, non-condensing. The system's compact design and low power consumption (max 150W) make it adaptable to various laboratory configurations, including mobile or field-deployable settings. All documentation, including user manuals, service records, and compliance certificates, is available in English and can be provided in digital format upon request.