Pre-owned / Used 2007 APPLIED BIOSYSTEMS StepOnePlus REAL TIME PCR THERMAL CYCLERS for Sale

Hardware Summary

Real-time PCR system with 96-well block, 0.2mL reaction volume, ±0.25°C temperature accuracy, 4-color multiplexing, USB 2.0/3.0 connectivity, compact benchtop design, 100-240V AC power in...

Software Summary

StepOne Software v2.3 with user management, data export to LIMS, FDA 21 CFR Part 11 compliant, audit trail, real-time amplification plots, melting curve analysis, multi-user access, netwo...

Accessories Summary

Compatible with MicroAmp Optical 96-Well Reaction Plates, MicroAmp Optical Caps, TaqMan Gene Expression Assays, Power SYBR Green PCR Master Mix, Applied Biosystems 7500/StepOne calibratio...

Hardware

The Applied Biosystems StepOnePlus Real-Time PCR System is a benchtop thermal cycler designed for quantitative nucleic acid amplification and detection. The system features a 96-well 0.2mL block with independent Peltier-based thermal control, achieving precise temperature uniformity of ±0.25°C across all wells. The optical system employs four high-performance LED excitation sources with corresponding filters for 4-color multiplexing (FAM, VIC, NED, ROX), enabling simultaneous detection of up to four distinct fluorophores per reaction. The instrument includes a high-resolution CCD camera for real-time fluorescence monitoring with a dynamic range exceeding 5 logs. Connectivity options include dual USB 2.0/3.0 ports for data transfer and peripheral device integration, alongside an Ethernet port for networked laboratory environments. The system operates on 100-240V AC input with auto-sensing voltage adjustment and consumes 150W during peak operation. Physical dimensions are 33.

Software / Upgrade / OS / Applications / Worklist

The StepOne Software v2.3 is a comprehensive, FDA 21 CFR Part 11-compliant platform designed for real-time PCR data acquisition, analysis, and reporting. The software supports multi-user environments with role-based access control, ensuring compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines. Data acquisition features include real-time amplification plots, endpoint fluorescence analysis, and melting curve analysis with derivative melting peak detection. The software supports absolute and relative quantification methods, including comparative Cq (ΔΔCq) analysis for gene expression studies. Advanced features include baseline correction algorithms, automatic threshold setting, and noise reduction filters to enhance data accuracy. The software integrates seamlessly with Laboratory Information Management Systems (LIMS) via CSV, XML, or direct database connectivity, enabling automated data transfer and reducing manual entry errors.

Accessories

The StepOnePlus system is compatible with a comprehensive ecosystem of accessories and consumables designed to optimize performance and workflow efficiency. Core consumables include MicroAmp Optical 96-Well Reaction Plates (Cat. No. 4326935), which feature a proprietary optical design for enhanced fluorescence detection and reduced well-to-well variability. MicroAmp Optical Caps (Cat. No. 4323032) provide a secure seal to prevent evaporation and contamination, with low autofluorescence properties. Applied Biosystems offers a range of validated PCR master mixes, including Power SYBR Green PCR Master Mix (Cat. No. 4367659) for dye-based detection and TaqMan Gene Expression Assays (Cat. No. 4331182) for probe-based quantification, both optimized for the StepOnePlus platform. Calibration standards include the Applied Biosystems 7500/StepOne Calibration Kit (Cat. No. 4346903), which ensures optical and thermal performance verification. Additional accessories include USB cables (Cat. No.

Warranty Inclusions & Exclusions

This pre-owned Applied Biosystems StepOnePlus Real-Time PCR System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering all mechanical, optical, and electronic components, including the thermal block, optical detection system, and control electronics. The warranty includes on-site service for critical failures, with a guaranteed response time of 48 hours for emergency repairs. For non-critical issues, remote troubleshooting and software support are provided within 24 hours. The warranty covers parts and labor for all manufacturer-recommended maintenance, including thermal calibration, optical alignment, and software updates. Extended warranty options are available at an additional cost, including a 24-month comprehensive package that covers accidental damage, extended calibration intervals, and priority technical support.

Service History

This Applied Biosystems StepOnePlus Real-Time PCR System has undergone rigorous service and maintenance to ensure optimal performance and reliability. The device was last serviced in Q2 2023 by an Applied Biosystems-certified technician, including a full thermal calibration, optical alignment verification, and software update to v2.3. Prior to this, the system underwent a comprehensive performance qualification in Q4 2022, including sensitivity testing with serial dilutions of a known target (limit of detection: 10 copies/µL), specificity testing with non-target sequences, and reproducibility testing across multiple runs (CV < 2%). The thermal block was inspected for uniformity, with temperature mapping confirming ±0.25°C accuracy across all 96 wells. The optical system was calibrated using Applied Biosystems' fluorescence standards, with cross-talk measurements confirming < 0.1% between channels.

Packing List

The Applied Biosystems StepOnePlus Real-Time PCR System is packaged for secure transportation and includes all necessary components for immediate installation and operation. The shipment includes the main unit (StepOnePlus Real-Time PCR System) with integrated thermal block and optical detection system, pre-installed StepOne Software v2.3 on a dedicated data acquisition computer, and a power cable compatible with the destination country's voltage standards. The package also includes a USB 2.0/3.0 data transfer cable for connecting the instrument to a PC or network, a set of MicroAmp Optical Caps (1000 units) for sealing reaction plates, and a calibration kit for optical and thermal performance verification. Additional accessories include a user manual in digital and printed formats, a quick start guide, and a safety data sheet (SDS) for all included components. The shipment is packed in a double-walled corrugated cardboard box with internal foam padding to protect against shocks and...

Additional Information

The Applied Biosystems StepOnePlus Real-Time PCR System is a CE-IVD and FDA 510(k)-cleared device, designed for in vitro diagnostic (IVD) use in clinical laboratories, research institutions, and biopharmaceutical settings. The system complies with multiple international regulatory standards, including ISO 13485 (Quality Management Systems), ISO 14971 (Risk Management), and IEC 61010-1 (Safety Requirements for Electrical Equipment). The device is listed under the FDA's Class II medical device category, with 510(k) clearance for applications such as pathogen detection, genetic testing, and gene expression analysis. The StepOnePlus is compatible with a wide range of clinical applications, including infectious disease diagnostics (e.g., SARS-CoV-2, influenza, HIV), oncology (e.g., mutation detection in EGFR, KRAS), and pharmacogenomics (e.g., CYP450 genotyping). The system supports both qualitative and quantitative PCR workflows, with validated protocols for common assays such as TaqMan Pathogen Detection Kits and TaqMan Gene Expression Assays. The device is designed for integration with hospital and laboratory information systems (HIS/LIS) via HL7 or FHIR standards, enabling automated data transfer and reducing manual entry errors. The StepOne Software v2.3 includes built-in compliance features for FDA 21 CFR Part 11, GDPR, and HIPAA, ensuring data integrity and patient confidentiality. The system is compatible with Applied Biosystems' QuantStudio Design & Analysis Software for advanced data analysis, including machine learning-based interpretation of amplification curves. The device has been validated for use in Good Clinical Laboratory Practice (GCLP) environments, with documented performance metrics for sensitivity, specificity, and reproducibility. The StepOnePlus is part of Applied Biosystems' global service network, with certified technicians available in over 50 countries for on-site support and training.