Pre-owned / Used 2010 EPPENDORF AG Mastercycler Nexus THERMAL CYCLERS for Sale

Hardware Summary

96-well block, 0.2 ml tube capacity, gradient function, Peltier-based thermoelectric control, ±0.2°C temperature accuracy, 0.1°C temperature resolution, 5°C to 100°C range, 300 W power, U...

Software Summary

Mastercycler Nexus Software v2.8, intuitive touchscreen GUI, real-time temperature monitoring, gradient optimization, protocol storage for 500 methods, user management, audit trail, GLP/G...

Accessories Summary

Compatible with Eppendorf 0.2 ml tubes, 96-well plates, PCR strips, heated lids, universal block adapters, Eppendorf SmartBlock, calibration tools, service kits, warranty extensions, Eppe...

Hardware

The Eppendorf Mastercycler Nexus is a high-performance thermal cycler designed for precise DNA amplification, PCR, qPCR, and other molecular biology applications. The unit features a 96-well block with 0.2 ml tube capacity, utilizing Peltier-based thermoelectric technology for rapid and accurate temperature cycling. The temperature control system achieves ±0.2°C accuracy and 0.1°C resolution across a range of 5°C to 100°C, ensuring reproducibility in critical experiments. The device is equipped with a gradient function, allowing simultaneous optimization of annealing temperatures across 12 columns, reducing the need for multiple runs. Connectivity options include USB and RS-232 ports for seamless integration with laboratory information systems (LIS) and data management software. The thermal cycler operates at 300 W power consumption and weighs 12 kg, with a compact footprint of 30x40x25 cm, making it suitable for benchtop use in clinical and research laboratories.

Software / Upgrade / OS / Applications / Worklist

The Mastercycler Nexus is powered by Eppendorf's proprietary Nexus Software v2.8, a user-friendly graphical interface designed for intuitive protocol programming and execution. The software supports real-time temperature monitoring, allowing users to visualize and adjust cycling parameters dynamically. Gradient optimization tools enable parallel testing of multiple annealing temperatures, streamlining assay development. Protocols can be stored for up to 500 methods, with user management features to enforce access controls and audit trails for GLP/GMP compliance. Data export capabilities include CSV and PDF formats, facilitating integration with laboratory information systems (LIS) and electronic health records (EHR). The software is compatible with Windows and macOS operating systems and supports remote monitoring via Eppendorf Connect, enabling supervisors to oversee multiple devices across a network.

Accessories

The Eppendorf Mastercycler Nexus supports a comprehensive ecosystem of accessories to enhance functionality and compatibility with various laboratory workflows. Compatible consumables include Eppendorf 0.2 ml PCR tubes, 96-well plates, and PCR strips, all designed for optimal thermal transfer and chemical resistance. The device accommodates Eppendorf SmartBlock adapters, allowing seamless transition between different tube formats without compromising performance. Heated lids are available for applications requiring precise temperature control, such as qPCR and isothermal amplification. Universal block adapters enable the use of third-party consumables, expanding the device's versatility. Calibration tools and service kits are available to maintain peak performance, including temperature verification modules and block replacement kits. Eppendorf offers certified accessories that meet ISO 9001 and CE standards, ensuring compatibility with regulatory requirements.

Warranty Inclusions & Exclusions

This pre-owned Eppendorf Mastercycler Nexus is offered with a comprehensive warranty package tailored to meet the needs of clinical and research laboratories. The seller provides a 12-month limited warranty covering defects in materials and workmanship, with options for extended coverage at additional cost. The warranty includes on-site service for major components, such as the Peltier elements and thermal block, ensuring minimal downtime. Technical support is available via phone and email, with a dedicated service team trained by Eppendorf AG. The warranty covers parts and labor, with expedited shipping for replacement components where necessary. A full refund or return policy is available at extra cost, subject to inspection and verification of the device's condition. The seller adheres to Eppendorf's service protocols, including calibration and performance validation, to ensure the device meets original specifications.

Service History

The Eppendorf Mastercycler Nexus has undergone rigorous service and maintenance to ensure optimal performance and reliability. The device's service history includes annual calibration checks using NIST-traceable thermometers to verify temperature accuracy within ±0.2°C. Preventive maintenance has been performed every six months, including cleaning of the thermal block, inspection of electrical connections, and lubrication of moving parts. The Peltier elements have been tested for thermal efficiency, with replacement conducted as needed to maintain consistent performance. The device's software has been updated to the latest version, with all security patches and compliance updates applied. A full diagnostic test suite has been executed, confirming the functionality of all hardware components, including the gradient feature, connectivity ports, and user interface. The device has been serviced by Eppendorf-certified technicians, with all service records documented in compliance with IS...

Packing List

The Eppendorf Mastercycler Nexus is supplied with a complete packing list to ensure a seamless setup and integration into the laboratory environment. The package includes the thermal cycler unit, power cable, USB cable, and RS-232 cable for connectivity. A user manual, quick-start guide, and software installation CD are provided for reference. The accessories kit contains a set of Eppendorf 0.2 ml PCR tubes (1000 units), a 96-well plate adapter, and a heated lid. Calibration certificates and service records are included, along with a warranty registration card. The device is shipped in a reinforced cardboard box with internal foam padding to prevent damage during transit. All components are secured with plastic straps and labeled for easy identification. The packing list is cross-referenced with the serial number to ensure traceability and compliance with regulatory requirements. A packing slip is provided for customs and shipping purposes, detailing the contents and value of the sh...

Additional Information

The Eppendorf Mastercycler Nexus is a Class IIa medical device certified under the EU Medical Device Regulation (MDR) 2017/745 and complies with FDA 21 CFR Part 820 Quality System Regulation. The device is manufactured in accordance with ISO 13485 standards for medical device quality management systems, ensuring adherence to global regulatory requirements. The thermal cycler is designed for use in clinical laboratories, research facilities, and biopharmaceutical applications, supporting a wide range of molecular biology techniques, including PCR, qPCR, DNA sequencing, and genotyping. The device's gradient function enables parallel optimization of annealing temperatures, reducing the time and resources required for assay development. The Mastercycler Nexus is compatible with Eppendorf's range of consumables, including PCR tubes, plates, and strips, all of which are manufactured under stringent quality control processes. The device features a robust aluminum thermal block with uniform heat distribution, minimizing edge effects and ensuring reproducible results. The Nexus Software v2.8 supports GLP/GMP compliance, with audit trails, user management, and data export capabilities for seamless integration with laboratory information systems (LIS). The device is equipped with safety features such as over-temperature protection, emergency shutdown protocols, and a low-voltage power supply to ensure operator safety. The Mastercycler Nexus has been validated for use in clinical diagnostics, including applications such as pathogen detection, genetic testing, and forensic analysis. The device's compact footprint and lightweight design make it ideal for benchtop use in space-constrained laboratories. The seller provides comprehensive documentation, including calibration certificates, service records, and compliance declarations, to support regulatory submissions and audits.