Pre-owned / Used 2019 THERMO FISHER QuantStudio 5 THERMAL CYCLERS for Sale

Hardware Summary

96-well thermal cycler with ±0.2°C temperature accuracy, 0.1°C uniformity, 384-well block option, USB/ethernet connectivity, compact benchtop design, active cooling to 4°C, real-time fluo...

Software Summary

QuantStudio Design & Analysis Software v1.5.1, FDA 21 CFR Part 11 compliant, LIMS integration, multi-user access control, automated data export, real-time PCR analysis, melt curve analysi...

Accessories Summary

Compatible with MicroAmp Optical 96-Well Reaction Plates, MicroAmp Optical 384-Well Reaction Plates, MicroAmp Adhesive Film, optical caps, calibration standards, validation kits, PCR-grad...

Hardware

The Thermo Fisher QuantStudio 5 Real-Time PCR System is a benchtop thermal cycler designed for high-throughput quantitative PCR applications. The system features a 96-well 0.2 mL block with optional 384-well compatibility, utilizing Peltier-based thermal control for precise temperature regulation across the entire block. Temperature accuracy is maintained at ±0.2°C with uniformity of ±0.1°C, ensuring consistent amplification across all wells. The instrument incorporates active cooling to 4°C, enabling rapid thermal cycling and reduced run times. Connectivity options include USB 2.0 for peripheral devices and Ethernet for network integration, facilitating data transfer to laboratory information systems (LIS) and electronic health records (EHR). The system operates on universal voltage (100-240V, 50/60Hz) with a power consumption of 150W, and includes a 7-inch capacitive touchscreen for intuitive user interaction.

Software / Upgrade / OS / Applications / Worklist

The QuantStudio Design & Analysis Software v1.5.1 is a comprehensive, FDA 21 CFR Part 11-compliant platform designed for real-time PCR data acquisition, analysis, and reporting. The software supports multi-user environments with role-based access control, ensuring compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines. Key features include real-time fluorescence monitoring with adjustable gain settings, automated baseline correction, and advanced threshold cycle (Ct) determination algorithms. The software supports multiple assay types, including endpoint genotyping, allelic discrimination, melt curve analysis, and digital PCR applications. Data export capabilities include CSV, PDF, and LIMS-compatible formats, with automated data transfer protocols for integration with laboratory information management systems (LIMS) such as Thermo Fisher's Connect platform.

Accessories

The QuantStudio 5 system is compatible with a comprehensive ecosystem of Thermo Fisher Scientific consumables and accessories designed to optimize performance and workflow efficiency. Primary consumables include MicroAmp Optical 96-Well Reaction Plates (Part No. N8010560) and MicroAmp Optical 384-Well Reaction Plates (Part No. 4309849), both featuring proprietary optical clarity for maximum fluorescence signal detection. Adhesive films (Part No. 4311971) and optical caps (Part No. 4323032) are available for plate sealing, with options for standard and ultra-clear variants to minimize background fluorescence. Calibration standards (Part No. 4485694) and validation kits (Part No. A39129) are provided for routine performance verification, including thermal ramp rate testing, block uniformity assessment, and fluorescence sensitivity calibration. The system supports a range of PCR-grade reagents, including TaqMan assays (Part No. 4331182), SYBR Green Master Mix (Part No.

Warranty Inclusions & Exclusions

The QuantStudio 5 system is offered with a comprehensive warranty package designed to provide maximum protection and operational assurance. The base warranty includes a 12-month coverage period from the date of installation, covering all manufacturing defects and component failures under normal operating conditions. This warranty includes parts replacement, labor costs, and technical support for troubleshooting and repair. Extended warranty options are available for an additional fee, extending coverage to 24 or 36 months, with optional on-site service agreements for immediate response in clinical environments. The warranty covers the thermal cycler block, Peltier elements, optical detection system, touchscreen interface, and electronic components, excluding consumables and accessories. Service response time is guaranteed within 48 business hours for standard warranty coverage, with priority service for extended warranties.

Service History

The QuantStudio 5 system has undergone rigorous preventive maintenance and service interventions to ensure optimal performance and compliance with clinical standards. The service history includes quarterly thermal calibration procedures using NIST-traceable temperature standards, with documented verification of ±0.2°C accuracy and ±0.1°C uniformity across the 96-well block. Block uniformity testing is performed using a calibrated thermal mapping system, with results recorded in compliance with ISO 13485 and FDA 21 CFR Part 820 requirements. Preventive maintenance includes cleaning of the optical detection system, lubrication of mechanical components, and inspection of electrical connections. The Peltier elements have been tested for thermal cycling efficiency, with performance metrics recorded to ensure compliance with manufacturer specifications. Software updates have been applied regularly, including patches for security vulnerabilities and enhancements for new assay protocols.

Packing List

The QuantStudio 5 system is shipped in a climate-controlled, shock-absorbent packaging solution designed to protect sensitive components during transit. The outer packaging consists of a double-walled corrugated cardboard box with reinforced corners and internal foam padding, meeting ISTA 3A shipping standards for medical equipment. The inner packaging includes a custom-molded foam insert tailored to the system's dimensions, with cutouts for the thermal cycler, power adapter, and accessories. The thermal cycler unit is secured with anti-static foam and plastic wrap to prevent electrostatic discharge (ESD) damage. The packing list includes the QuantStudio 5 thermal cycler (Model No. A39129), universal power adapter (Part No. 4484757) with international plug adapters, 7-inch capacitive touchscreen, USB 2.0 cable (Part No. 4484754), Ethernet cable (Part No. 4484756), and installation guide (Part No. 4485697). Additional components include the service toolkit (Part No.

Additional Information

The QuantStudio 5 Real-Time PCR System is a Class IIa medical device certified under the European In Vitro Diagnostic Directive (IVDD 98/79/EC) and complies with the In Vitro Diagnostic Regulation (IVDR 2017/746), ensuring suitability for clinical diagnostic applications. The system is registered with the U.S. Food and Drug Administration (FDA) under 510(k) clearance (K183283) for use in human in vitro diagnostic procedures, including pathogen detection, genetic testing, and oncology applications. The device adheres to ISO 13485:2016 quality management system standards and is manufactured in a facility certified to ISO 9001:2015. Clinical applications include infectious disease diagnostics (e.g., SARS-CoV-2, influenza, HIV), genetic testing (e.g., BRCA1/2, CFTR), oncology (e.g., liquid biopsy, minimal residual disease monitoring), and pharmacogenomics (e.g., drug metabolism genotyping). The system supports compliance with the Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) accreditation requirements, with validation protocols for use in high-complexity laboratories. Integration capabilities include HL7 and FHIR standards for seamless data exchange with hospital information systems (HIS), electronic medical records (EMR), and laboratory information systems (LIS). The system is compatible with Thermo Fisher's Applied Biosystems Connect platform, enabling cloud-based data storage, remote monitoring, and multi-site data aggregation for research and clinical networks. Environmental specifications include operating temperatures of 15-30°C, humidity levels of 20-80% non-condensing, and altitude tolerance up to 2000 meters. The system meets electromagnetic compatibility (EMC) standards, including IEC 61326-1 for industrial environments and IEC 60601-1-2 for medical electrical equipment.