Pre-owned / Used 2017 ROCHE LightCycler 480 II, 96 THERMAL CYCLERS for Sale

Hardware Summary

96-well thermal cycler with 4-channel multiplexing, 0.1°C temperature accuracy, 384-well compatibility, Peltier-based heating/cooling, USB/Ethernet connectivity, compact benchtop design, ...

Software Summary

LightCycler 480 Software Version 1.5 with advanced data analysis modules, multi-user access control, LIMS integration, automated data export to CSV/PDF, real-time PCR quantification, melt...

Accessories Summary

Compatible with Roche LightCycler 480 Multiwell Plates (96-well and 384-well), LightCycler 480 Sealing Foils, LightCycler 480 Optical Caps, LightCycler 480 Control Kit, LightCycler 480 Ca...

Hardware

The Roche LightCycler 480 II thermal cycler is a high-performance, benchtop real-time PCR system designed for both research and clinical diagnostics applications. The instrument features a 96-well block with independent Peltier-based heating and cooling systems, delivering precise temperature control with a uniformity of ±0.25°C across all wells and a temperature accuracy of ±0.1°C. The system supports a temperature range from 4°C to 99°C with ramp rates up to 4.8°C/second, enabling rapid cycling protocols. The optical system incorporates four high-intensity LEDs (465nm, 533nm, 618nm, 660nm) with corresponding photomultiplier tube detectors, facilitating multiplex fluorescence detection for up to four distinct fluorophores simultaneously. The instrument includes a built-in thermal calibration system for automated performance verification, ensuring consistent results over extended use. The compact footprint (450mm x 400mm x 450mm) and integrated touchscreen interface enhance usabilit...

Software / Upgrade / OS / Applications / Worklist

The LightCycler 480 Software Version 1.5 is a comprehensive, FDA 21 CFR Part 11-compliant platform designed for real-time PCR data acquisition, analysis, and reporting. The software operates on Windows 10/11 (64-bit) and supports multi-user environments with role-based access control. Key features include automated plate setup with pre-configured protocols for common applications such as gene expression analysis, SNP genotyping, and pathogen detection. The software supports four-color multiplexing with real-time fluorescence monitoring, enabling simultaneous detection of multiple targets within a single reaction. Advanced analysis modules include absolute and relative quantification, comparative Ct (ΔΔCt) method, end-point genotyping, and high-resolution melting (HRM) curve analysis for mutation scanning and genotyping applications. The platform offers automated data export to CSV, PDF, and XML formats, with direct integration capabilities for laboratory information systems (LIS) vi...

Accessories

The Roche LightCycler 480 II system is supported by a comprehensive ecosystem of accessories and consumables designed to optimize performance and ensure regulatory compliance. Roche offers validated multiwell plates in 96-well and 384-well formats, including standard, semi-skirted, and full-skirted configurations to accommodate various thermal cycler models and applications. The LightCycler 480 Sealing Foils and Optical Caps provide secure, optically clear sealing for PCR reactions, minimizing evaporation and ensuring consistent fluorescence detection. The LightCycler 480 Control Kit includes positive and negative controls for routine performance verification, while the Calibration Kit enables automated thermal and optical calibration to maintain instrument accuracy. The system is compatible with Roche's proprietary reagents, including the FastStart DNA Master SYBR Green I kit for gene expression analysis and the Universal Probe Library for probe-based assays.

Warranty Inclusions & Exclusions

This pre-owned Roche LightCycler 480 II thermal cycler is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering all parts and labor associated with defects in materials and workmanship, commencing from the date of delivery. The warranty includes on-site service by certified technicians for critical components such as the Peltier elements, optical system, and thermal calibration modules. The warranty also covers software updates and technical support for the duration of the warranty period. Extended warranty options are available for an additional fee, extending coverage to 24 or 36 months, with options for accelerated response times and priority scheduling. The warranty excludes consumables, accessories, and damage resulting from misuse, improper installation, or unauthorized modifications.

Service History

This Roche LightCycler 480 II thermal cycler has undergone rigorous preventive maintenance and service procedures in accordance with Roche Diagnostics' service protocols. The service history includes annual calibration and performance verification by Roche-certified engineers, ensuring compliance with ISO 17025 standards for temperature uniformity, ramp rates, and fluorescence detection accuracy. The instrument has been serviced with genuine Roche parts, including replacement of Peltier elements, optical filters, and thermal calibration modules as required. The service records document routine cleaning of the optical system, lubrication of mechanical components, and verification of electrical safety in accordance with IEC 61010-1 standards. The system has been subjected to performance testing using Roche's proprietary control kits, with all results falling within specified tolerances. The service history includes records of software updates and patches applied to maintain compliance...

Packing List

The Roche LightCycler 480 II thermal cycler is supplied with a complete packing list to ensure a seamless installation and setup process. The package includes the thermal cycler unit with integrated touchscreen interface, power cable (100-240V AC, 50/60Hz), USB 2.0 cable, Ethernet cable (Cat 5e), installation CD containing the LightCycler 480 Software Version 1.5, quick reference guide, user manual in English, calibration certificate, performance verification report, and a certificate of conformity. The package also includes a set of LightCycler 480 Sealing Foils (100 sheets), a set of LightCycler 480 Optical Caps (100 units), and a LightCycler 480 Control Kit (positive and negative controls). Additional accessories include a spare fuse kit, a set of calibration tools, a maintenance kit containing cleaning supplies and lubricants, and a set of international power adapters. The package is shipped in a reinforced cardboard box with internal foam padding to protect the instrument durin...

Additional Information

The Roche LightCycler 480 II thermal cycler is a Class IIa medical device under the European Medical Device Regulation (MDR 2017/746) and is FDA 510(k) cleared for in vitro diagnostic use in the United States. The instrument is CE IVD marked, indicating compliance with the European In Vitro Diagnostic Directive 98/79/EC, and is manufactured in accordance with ISO 13485 quality management standards. The system is designed for use in clinical diagnostics, research laboratories, and academic institutions, supporting applications such as gene expression analysis, pathogen detection, SNP genotyping, and high-resolution melting curve analysis. The LightCycler 480 II is compatible with a wide range of PCR reagents and consumables, including Roche's proprietary FastStart DNA Master SYBR Green I kit and Universal Probe Library, as well as third-party reagents validated for use with the system. The instrument supports integration with laboratory information systems (LIS) via HL7 or ASTM protocols, enabling seamless data transfer and automated reporting. The system is designed to meet the stringent requirements of clinical laboratories, including compliance with CLIA (Clinical Laboratory Improvement Amendments) and CAP (College of American Pathologists) standards. The thermal cycler is equipped with advanced safety features, including over-temperature protection, automatic shut-off in case of power failure, and electrical safety compliance with IEC 61010-1. The instrument is also designed to minimize cross-contamination risks through the use of sealed reaction vessels and optimized thermal cycling protocols. The LightCycler 480 II has been validated for use in a variety of clinical applications, including infectious disease testing, oncology research, genetic testing, and food safety analysis. The system is supported by Roche Diagnostics' global service network, with certified technicians available for on-site maintenance, calibration, and troubleshooting.