Pre-owned / Used 2021 BIO-RAD CFX Opus 96 THERMAL CYCLERS for Sale

Hardware Summary

96-well capacity, 0.2ml tube compatibility, 0.5°C temperature accuracy, gradient feature, USB/Ethernet connectivity, compact benchtop design, LED calibration indicators, Peltier-based the...

Software Summary

CFX Manager Software v3.1, FDA 510(k) cleared, CFR Part 11 compliant, real-time PCR analysis, multi-user access control, GLP/GMP compliant data export, LIMS integration, touchscreen inter...

Accessories Summary

Compatible with 0.2ml tubes/plates, calibration kits, dust covers, USB cables, Ethernet cables, CFX Opus 96 Quick Start Guide, Bio-Rad certified accessories, GLP-compliant documentation t...

Hardware

The Bio-Rad CFX Opus 96 Real-Time PCR Detection System is a high-performance thermal cycler designed for quantitative and qualitative PCR applications in clinical and research laboratories. The system features a 96-well block with 0.2ml tube compatibility, ensuring compatibility with standard PCR consumables. The thermal block employs Peltier technology for precise temperature control with a uniformity of ±0.2°C across all wells, enabling consistent and reproducible results. The instrument operates within a temperature range of 4°C to 100°C, with a ramp rate of up to 5°C/second, significantly reducing run times for PCR protocols. The integrated gradient feature allows for the optimization of annealing temperatures across 12 distinct zones, enhancing assay development efficiency. Connectivity options include USB 2.0 and Ethernet (10/100), facilitating seamless data transfer and remote monitoring capabilities.

Software / Upgrade / OS / Applications / Worklist

The CFX Opus 96 is powered by Bio-Rad's CFX Manager Software v3.1, a comprehensive platform designed for real-time PCR data acquisition, analysis, and reporting. The software is compliant with 21 CFR Part 11, ensuring secure electronic records and signatures for regulated environments. It supports multi-user access control with role-based permissions, enhancing data integrity and audit trail capabilities. The software features advanced analysis tools, including baseline subtraction, threshold cycle (Ct) determination, and melting curve analysis, enabling precise quantification and genotyping. Data export is fully compatible with GLP/GMP standards, supporting formats such as CSV, PDF, and XML for seamless integration with laboratory information systems (LIS/LIMS). The touchscreen interface provides an intuitive user experience, with customizable dashboard layouts for monitoring multiple runs simultaneously.

Accessories

The CFX Opus 96 thermal cycler is compatible with a comprehensive range of Bio-Rad and third-party accessories to enhance functionality and workflow efficiency. Standard accessories include 96-well 0.2ml PCR plates and tubes, calibration kits for temperature verification, and dust covers for instrument protection. Bio-Rad offers certified accessories such as the CFX Opus 96 Optical Reaction Module, which includes the excitation and detection systems for fluorescence-based PCR applications. Additional accessories include the CFX Opus 96 Gradient Thermal Cycler Block for enhanced assay optimization, and the CFX Opus 96 96-Well Plate Sealing Film Applicator for secure sample containment. The system supports a variety of optical filters for multiplexing applications, including FAM, HEX, ROX, and Cy5, ensuring compatibility with a wide range of fluorophores. For data management, Bio-Rad provides GLP-compliant documentation templates and validation tools to streamline compliance reporting...

Warranty Inclusions & Exclusions

The Bio-Rad CFX Opus 96 thermal cycler is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The standard warranty includes a 12-month parts and labor coverage from the date of installation, with options for extended warranties up to 36 months. Bio-Rad's warranty covers defects in materials and workmanship, ensuring prompt repair or replacement of faulty components. The warranty also includes access to Bio-Rad's global technical support team, available 24/7 for troubleshooting and guidance. For customers requiring additional coverage, Bio-Rad offers premium service plans that include priority response times, annual preventive maintenance, and calibration services. The warranty is transferable, providing added value for resale or relocation of the instrument. Bio-Rad's service technicians are factory-trained and certified, ensuring high-quality repairs and maintenance.

Service History

The CFX Opus 96 thermal cycler has been meticulously maintained and serviced to ensure optimal performance and reliability. The instrument's service history includes annual preventive maintenance by Bio-Rad-certified technicians, with detailed records of all inspections, calibrations, and repairs. The thermal block has been calibrated using NIST-traceable temperature standards, ensuring accuracy within ±0.2°C across all wells. The Peltier modules have been tested for thermal uniformity and ramp rate performance, with all results documented in compliance with ISO 17025 standards. The optical detection system has been verified for fluorescence sensitivity and signal-to-noise ratio, with calibration records confirming compliance with manufacturer specifications. The instrument's software has been updated to the latest version, including all regulatory-compliant patches and security updates.

Packing List

The CFX Opus 96 thermal cycler is supplied with a complete packing list to ensure a seamless installation and setup process. The standard packing list includes the main instrument unit, power cable, USB cable, Ethernet cable, and a quick start guide. Additional components include the optical reaction module, calibration kit (temperature verification standards), dust cover, and a set of 96-well 0.2ml PCR plates for initial testing. The packing list also includes a CD-ROM with the CFX Manager Software v3.1 installation files, user manuals in PDF format, and regulatory compliance documentation. A USB flash drive containing pre-configured PCR protocols and validation templates is included for immediate use. The packing list is accompanied by a detailed inventory checklist to verify all components upon receipt. The instrument is shipped in a reinforced cardboard box with internal foam padding to prevent damage during transit.

Additional Information

The Bio-Rad CFX Opus 96 Real-Time PCR Detection System is a state-of-the-art thermal cycler designed for high-throughput, quantitative PCR applications in clinical diagnostics, research, and biopharmaceutical industries. The instrument is FDA 510(k) cleared and CE marked, ensuring compliance with global regulatory standards for in vitro diagnostic (IVD) use. It is also compliant with ISO 13485:2016, ISO 14971:2019, and IEC 62304:2015, demonstrating Bio-Rad's commitment to quality management and risk management systems. The CFX Opus 96 supports a wide range of PCR applications, including gene expression analysis, genotyping, mutation detection, and pathogen identification, making it a versatile tool for laboratories requiring precision and reliability. The instrument's gradient feature enables the optimization of annealing temperatures across 12 distinct zones, reducing the need for multiple runs and accelerating assay development. The CFX Manager Software v3.1 is fully compliant with 21 CFR Part 11, ensuring secure electronic records and signatures for regulated environments such as clinical diagnostics and pharmaceutical research. The software supports multi-user access control, audit trails, and data encryption, enhancing data integrity and compliance with GLP/GMP standards. The instrument is compatible with Bio-Rad's PrimePCR™ assays, which are pre-optimized for use with the CFX Opus 96, ensuring reliable and reproducible results. Additionally, the system supports third-party PCR plates and reagents, providing flexibility for laboratories with diverse workflows. The CFX Opus 96 is designed for seamless integration with laboratory information systems (LIS/LIMS) via Ethernet connectivity, enabling automated data transfer and reducing manual errors. The instrument's compact benchtop design and low power consumption make it ideal for laboratories with limited space or resources.