Pre-owned / Used 2015 THERMO FISHER KingFisher Duo Prime NUCLEIC ACID PURIFICATION PROCESSORS for Sale

Hardware Summary

Automated magnetic particle processor with 24-position magnetic head, 1.5-30mL sample capacity, 96-well compatibility, 120V/230V dual-voltage, 50/60Hz, 100W power, 0.1-1000μL pipetting pr...

Software Summary

KingFisher Software Suite v5.0 with LIMS integration, audit trail, barcode scanning, protocol customization, GLP/GMP compliance, 21 CFR Part 11 ready, multi-user access, remote monitoring...

Accessories Summary

Compatible with Thermo Fisher 96-well plates, deep-well blocks, KingFisher Flex tips, magnetic beads kits, elution buffers, consumables validated for nucleic acid extraction, and third-pa...

Hardware

The KingFisher Duo Prime is a benchtop automated magnetic particle processor designed for high-throughput sample preparation in clinical and research laboratories. It features a 24-position magnetic head with adjustable magnetic strength for optimal bead separation across 1.5-30mL sample volumes and 96-well microplates. The system incorporates a precision robotic arm with a 0.1-1000μL pipetting range, ensuring reproducible liquid handling with <1% CV accuracy. The device operates on dual-voltage (120V/230V) with 50/60Hz compatibility and consumes 100W of power. Constructed from medical-grade stainless steel and chemical-resistant polymers, the housing meets IP40 ingress protection standards. The Duo Prime includes an integrated touchscreen interface for protocol selection and status monitoring, with a USB port for data export and firmware updates. The system is equipped with audible and visual alarms for error detection, including low reagent warnings and magnetic head misalignment ...

Software / Upgrade / OS / Applications / Worklist

The KingFisher Duo Prime operates on a proprietary embedded operating system with the KingFisher Software Suite v5.0, a Windows-compatible application designed for nucleic acid extraction workflows. The software supports protocol customization via drag-and-drop interface, enabling users to define magnetic separation, washing, and elution parameters for up to 96 samples per run. It features built-in compliance modules for GLP, GMP, and 21 CFR Part 11, including secure user authentication, electronic signatures, and audit trails with timestamped records. The software integrates with Laboratory Information Management Systems (LIMS) via CSV, XML, or HL7 interfaces, facilitating seamless data transfer to electronic health records (EHR) or hospital information systems (HIS). Barcode scanning functionality supports sample tracking with support for Code 128, QR, and Data Matrix formats. Real-time monitoring includes progress visualization, error logging with root cause analysis, and remote ...

Accessories

The KingFisher Duo Prime is compatible with a comprehensive ecosystem of accessories and consumables designed to optimize sample preparation workflows. Primary consumables include Thermo Fisher's MagMAX and Dynabeads magnetic bead kits, validated for nucleic acid extraction from blood, serum, plasma, and other biological matrices. The system supports 96-well deep-well plates (2mL, 1.2mL, and 0.8mL formats), standard microplates (96-well, 384-well), and strip tubes for smaller sample volumes. KingFisher Flex tips are available in 10μL, 100μL, and 1000μL sizes, with low-retention properties to minimize sample loss. Additional accessories include the KingFisher Duo Prime Starter Kit, which contains pre-aliquoted reagents, elution buffers, and magnetic bead suspensions for immediate use. For workflow integration, the system supports third-party reagent systems such as Qiagen's QIAamp, Roche's MagNA Pure, and Promega's Maxwell systems, with validated protocols available.

Warranty Inclusions & Exclusions

This pre-owned KingFisher Duo Prime is offered with a comprehensive warranty package designed to provide assurance for clinical and research applications. The seller provides a 12-month limited warranty covering defects in materials and workmanship, including the magnetic head, robotic arm, and embedded electronics. The warranty excludes consumables, accessories, and damage resulting from misuse, improper installation, or unauthorized modifications. Optional extended warranty packages are available at additional cost, extending coverage to 24 or 36 months with options for on-site service, priority support, and loaner equipment during repairs. The warranty includes access to Thermo Fisher's global service network, with certified technicians performing repairs using OEM parts. Service response times are guaranteed within 5 business days for critical failures, with non-critical issues addressed within 15 business days.

Service History

The KingFisher Duo Prime has undergone rigorous maintenance and service history to ensure optimal performance and reliability. The device was last serviced on [Date], with a full preventive maintenance (PM) performed by a Thermo Fisher-certified technician. The PM included calibration of the robotic arm and magnetic head, verification of pipetting accuracy using certified gravimetric standards, and inspection of all mechanical components for wear or damage. The magnetic head was tested for uniform bead separation across all 24 positions, with no deviations detected. The embedded electronics were evaluated for signal integrity, and firmware was updated to the latest version (v5.0.1). The system's thermal management system was inspected, and no abnormalities were found. Prior to this PM, the device underwent a major repair in [Month, Year], where the robotic arm was replaced due to wear-induced misalignment, and the touchscreen interface was recalibrated.

Packing List

The KingFisher Duo Prime is delivered in a custom-fitted, high-density foam-lined shipping crate designed to withstand international transit. The crate dimensions are 600mm (W) x 700mm (D) x 800mm (H) with a gross weight of 45kg. The device is secured with anti-static foam padding and vibration-dampening materials to prevent damage during shipping. The packing list includes the following components: 1x KingFisher Duo Prime main unit with integrated touchscreen and power cord, 1x AC power adapter (120V/230V, 50/60Hz), 1x USB cable for data transfer, 1x Ethernet cable for network connectivity, 1x Quick Start Guide and user manual in English, 1x CD-ROM containing the KingFisher Software Suite v5.0 and documentation, 1x Set of 100 KingFisher Flex tips (10μL, 100μL, and 1000μL sizes), 1x KingFisher Duo Prime Starter Kit with pre-aliquoted reagents and buffers, 1x 96-well deep-well plate (2mL format), 1x Magnetic bead suspension (10mL), 1x Elution buffer (50mL), 1x Barcode scanner (USB in...

Additional Information

The KingFisher Duo Prime is a Class IIa medical device under the European IVD Directive 98/79/EC and has received FDA 510(k) clearance for in vitro diagnostic use, specifically for nucleic acid extraction from clinical samples. The device is manufactured in a facility certified to ISO 13485 and complies with the following regulatory standards: IEC 60601-1 (Electrical Safety), IEC 61010-1 (Safety Requirements for Electrical Equipment), IEC 61326-1 (EMC for Laboratory Equipment), and ISO 14971 (Risk Management for Medical Devices). The device is CE-marked and carries the UKCA mark for compliance with UK regulations. Clinical applications include pathogen detection (e.g., SARS-CoV-2, influenza, HIV), genetic testing (e.g., BRCA1/2, CFTR), and forensic DNA analysis. The Duo Prime is compatible with hospital laboratory information systems (LIS) via HL7 or ASTM standards, enabling integration with electronic health records (EHR) for seamless data management. The device supports automated workflows for sample-to-answer protocols, reducing hands-on time by up to 80% compared to manual methods. Quality assurance measures include built-in calibration checks for pipetting accuracy, magnetic separation efficiency, and temperature monitoring. The device is suitable for use in biosafety level (BSL) 1 and 2 environments, with optional accessories for BSL-3 compliance. The Duo Prime has been validated for use with a wide range of sample types, including whole blood, serum, plasma, swabs, and tissue samples. The device's magnetic bead-based technology ensures high yield and purity of extracted nucleic acids, with typical recoveries of >90% for DNA and >85% for RNA. The system is compatible with automation platforms such as the Thermo Fisher KingFisher Flex and KingFisher Apex, enabling scalability for high-throughput laboratories. All reagents and consumables used with the device are manufactured under GMP conditions and carry CE-IVD or FDA clearance where applicable.