Pre-owned / Used 2011 BIO-RAD CFX96 THERMAL CYCLERS for Sale

Hardware Summary

96-well thermal cycler with 0.2°C temperature accuracy, 384-well compatibility, 5-color multiplexing, USB/ethernet connectivity, compact benchtop design, Peltier-based thermal control, gr...

Software Summary

CFX Manager software with FDA 21 CFR Part 11 compliance, LIMS integration, multi-user access control, automated data analysis, thermal gradient optimization, export to CSV/PDF, touchscree...

Accessories Summary

Compatible with all Bio-Rad PCR plates, optical caps, calibration kits, validation standards, CFX automation accessories, third-party plate adapters, maintenance kits, service tools, and ...

Hardware

The Bio-Rad CFX96 Real-Time PCR Detection System is a high-performance thermal cycler designed for quantitative PCR (qPCR) and end-point PCR applications. This pre-owned unit features a 96-well (0.2 mL) block with optional 384-well compatibility, utilizing a Peltier-based thermal control system for precise temperature regulation (±0.2°C) across the entire block. The system supports a temperature range of 4°C to 100°C with a maximum ramp rate of 5°C/second, enabling rapid cycling protocols. The optical system includes a 5-color multiplexing capability (excitation at 450–650 nm, emission at 500–700 nm), allowing simultaneous detection of multiple fluorophores for advanced multiplex assays. The instrument is equipped with a 7-inch touchscreen interface for intuitive operation and real-time data visualization. Connectivity options include USB 2.0, Ethernet, and optional Wi-Fi for seamless integration with laboratory information management systems (LIMS). The compact benchtop design (43.

Software / Upgrade / OS / Applications / Worklist

The CFX96 is powered by Bio-Rad’s CFX Manager Software, a comprehensive, user-friendly platform designed for real-time PCR data acquisition, analysis, and reporting. The software supports both Windows and macOS operating systems and is compliant with FDA 21 CFR Part 11, ensuring secure electronic records and signatures for regulated environments. Key features include automated baseline and threshold cycle (Ct) determination, melting curve analysis, relative quantification (ΔΔCt), and absolute quantification using standard curves. The software supports multi-user access control with role-based permissions, ensuring compliance with laboratory quality management systems. Data can be exported in multiple formats (CSV, PDF, Excel) for integration with LIMS or other laboratory information systems. Advanced features include thermal gradient optimization for primer design, pre-programmed protocols for common applications (e.g.

Accessories

The CFX96 thermal cycler is supported by a comprehensive ecosystem of Bio-Rad certified accessories and consumables, ensuring optimal performance and reproducibility. Key accessories include: (1) Hard-Shell® 96-well PCR plates and 384-well plates, designed for high-performance thermal transfer and compatibility with the CFX96 block; (2) Optical-quality PCR plate seals and caps, including adhesive seals, heat-seal films, and optically clear caps, to minimize evaporation and maximize signal detection; (3) Calibration and validation kits, including the CFX Calibration Kit and Validation Plate, for routine performance verification and compliance with ISO 17025 and CLIA standards; (4) Thermal gradient optimization kits, such as the PrimePCR™ Assay Validation Kit, for primer and probe optimization; (5) Automation accessories, including the CFX Automation System II for high-throughput plate loading and the CFX Deep Well Plate Adapter for compatibility with deep-well plates; (6) Service and...

Warranty Inclusions & Exclusions

This pre-owned Bio-Rad CFX96 thermal cycler is offered with a comprehensive warranty and support package to ensure long-term reliability and performance. The seller provides a 12-month limited warranty covering defects in materials and workmanship, with options for extended warranty coverage at an additional cost. The warranty includes on-site service by Bio-Rad certified technicians for critical components such as the Peltier modules, thermal block, and optical detection system. Remote technical support is available 24/7 via Bio-Rad’s global customer service network, with response times guaranteed within 24 hours for urgent issues. The warranty also covers software updates and technical training for up to 3 users, ensuring optimal utilization of the CFX Manager Software. Additional services include annual performance validation, calibration, and preventive maintenance to maintain compliance with ISO 13485 and CLIA standards.

Service History

This Bio-Rad CFX96 thermal cycler has undergone rigorous preventive maintenance and service history, ensuring optimal performance and compliance with clinical and research standards. The instrument was last serviced in Q2 2023 by a Bio-Rad certified technician, with the following maintenance activities performed: (1) Thermal block cleaning and calibration, ensuring uniform temperature distribution (±0.2°C) across all wells; (2) Peltier module inspection and performance testing, verifying efficient thermal cycling and minimal drift; (3) Optical calibration, including excitation and emission wavelength verification for all 5 detection channels (FAM, HEX, ROX, Cy5, Quasar 670); (4) Software update to the latest version of CFX Manager (v3.1), including FDA 21 CFR Part 11 compliance patches; (5) Electrical safety testing, confirming compliance with IEC 61010-1 and IEC 60601-1 standards; (6) Performance validation using Bio-Rad’s Validation Plate, confirming Ct values within ±0.

Packing List

The Bio-Rad CFX96 thermal cycler is delivered in a secure, climate-controlled packaging solution designed to protect the instrument during transit. The packing list includes: (1) Main unit: Bio-Rad CFX96 Real-Time PCR Detection System, including 96-well thermal block, optical detection system, touchscreen interface, and power supply; (2) Power cable: IEC C13 to local standard power cord; (3) USB cable: For connection to a computer or LIMS; (4) Ethernet cable: For network connectivity; (5) Quick start guide and user manual: In digital and printed formats; (6) Installation and calibration certificate: Signed by a Bio-Rad certified technician, confirming performance within specifications; (7) Performance validation report: Including Ct values, melting curve analysis, and optical calibration results; (8) Accessory kit: Including a set of optical-quality PCR plate seals, a calibration plate, and a cleaning kit for the thermal block and optical components; (9) Packing materials: Including...

Additional Information

The Bio-Rad CFX96 Real-Time PCR Detection System is a versatile and high-performance thermal cycler designed for a wide range of clinical and research applications, including quantitative PCR (qPCR), end-point PCR, genotyping, pathogen detection, gene expression analysis, and microbial identification. This pre-owned unit is manufactured by Bio-Rad, a global leader in life science and clinical diagnostics, and is compliant with stringent regulatory standards, including FDA 510(k) clearance for in vitro diagnostic (IVD) use, CE-IVD marking for European markets, and ISO 13485 certification for quality management systems. The CFX96 is compatible with a broad ecosystem of Bio-Rad and third-party reagents, including SYBR Green, TaqMan, and FRET-based assays, ensuring flexibility for diverse experimental designs. The instrument’s 5-color multiplexing capability allows for simultaneous detection of multiple targets in a single reaction, reducing sample volume requirements and improving throughput. The system supports advanced applications such as digital PCR (via third-party integration), high-resolution melting (HRM) analysis, and absolute quantification using standard curves. The CFX96 is designed for integration with laboratory information management systems (LIMS) and electronic health records (EHR), enabling seamless data transfer and compliance with clinical data standards such as HL7 and FHIR. The instrument is also compatible with Bio-Rad’s PrimePCR™ Assay Database, providing pre-validated assays for common targets, ensuring reproducibility and reducing assay development time. The CFX96 meets the requirements of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, ensuring high-quality data reporting. The system is suitable for use in clinical laboratories, research institutions, and biopharmaceutical settings, with applications ranging from infectious disease diagnostics to cancer research and personalized medicine...