Pre-owned / Used 2016 BIO-RAD QX200 AutoDG Droplet Digital PCR Molecular Diagnostics System for Sale

Hardware Summary

Automated droplet generation, 96-well compatibility, 2D barcode reader, 0.5-20 µL sample range, 0.2% CV droplet uniformity, CE-IVD, FDA-cleared

Software Summary

QuantaSoft™ Pro Software v1.7, LIMS integration, CFR 21 Part 11 compliance, multi-user access, real-time data export, automated QC reporting

Accessories Summary

AutoDG™ Cartridges, Droplet Generation Oil, ddPCR™ Supermix, 96-well PCR plates, consumables kit, barcode scanner, calibration standards

Hardware

The BIO-RAD QX200 AutoDG Droplet Digital PCR System is a fully automated, high-throughput platform designed for precise nucleic acid quantification. The system features an integrated AutoDG droplet generator with a 96-well plate capacity, enabling simultaneous processing of up to 96 samples with droplet volumes of 0.5-20 µL. The droplet generator achieves exceptional uniformity with a coefficient of variation (CV) of <0.2%, ensuring reproducible results across all wells. The system includes a 2D barcode reader for seamless sample tracking and integration with laboratory information systems (LIS). The QX200 droplet reader utilizes a dual-channel fluorescence detection system with excitation wavelengths of 488 nm and 532 nm, and emission detection at 520 nm and 640 nm, respectively. The optical system is calibrated using NIST-traceable standards to maintain accuracy. The instrument is equipped with a temperature-controlled sample holder (4-40°C) and a high-precision thermal cycling mo...

Software / Upgrade / OS / Applications / Worklist

The QX200 AutoDG system operates with QuantaSoft™ Pro Software v1.7, a comprehensive, user-friendly platform designed for droplet digital PCR (ddPCR) data acquisition and analysis. The software supports multi-user access with role-based permissions, ensuring compliance with CFR 21 Part 11 regulations for electronic records and signatures. It features automated data processing with real-time quality control (QC) checks, including droplet count validation, amplitude uniformity assessment, and noise detection. The software enables seamless integration with laboratory information management systems (LIMS) via HL7 or ASTM protocols, facilitating automated data transfer and sample tracking. Data export options include CSV, PDF, and XML formats, with support for third-party analysis tools such as Microsoft Excel and R. The software includes pre-configured assay templates for common applications, including gene expression analysis, copy number variation (CNV) detection, and rare mutation de...

Accessories

The QX200 AutoDG system is supported by a comprehensive ecosystem of consumables and accessories designed to optimize workflow efficiency and reproducibility. Key consumables include AutoDG Cartridges for droplet generation, Droplet Generation Oil for emulsion stabilization, and ddPCR Supermix for reaction setup. The system is compatible with 96-well PCR plates from multiple manufacturers, including Eppendorf twin.tec®, Thermo Fisher MicroAmp®, and Bio-Rad HSP9601 plates, ensuring flexibility in reagent selection. Additional accessories include the Droplet Reader Cartridge for fluorescence detection, the Droplet Generation Oil for Probes for probe-based assays, and the ddPCR™ Negative Control for contamination monitoring. The system also supports the use of barcode scanners (e.g., Honeywell Xenon 1900) for sample tracking and integration with laboratory information systems. Calibration standards, including the ddPCR™ Calibration Kit, are available for periodic performance verificati...

Warranty Inclusions & Exclusions

This pre-owned BIO-RAD QX200 AutoDG Droplet Digital PCR System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month warranty covering all mechanical, optical, and electronic components, including the droplet generator, thermal cycler, and fluorescence detection system. The warranty includes on-site service by certified BIO-RAD technicians, with a guaranteed response time of 48 hours for critical issues and 5 business days for non-critical repairs. The warranty covers parts and labor, with no additional fees for diagnostics or troubleshooting. Extended warranty options are available for an additional cost, including a 24-month or 36-month coverage plan with the same response times and service levels. The warranty also includes access to BIO-RAD’s global technical support network, providing 24/7 phone and email support for operational and troubleshooting inquiries.

Service History

The QX200 AutoDG system has undergone rigorous maintenance and service to ensure optimal performance and reliability. The system was installed new in 2022 and has been serviced annually by BIO-RAD-certified technicians in accordance with the manufacturer’s recommended maintenance schedule. Key service records include: (1) Preventive maintenance performed in March 2023, including calibration of the droplet generator, optical system, and thermal cycler, as well as cleaning of the fluorescence detection module. (2) Corrective maintenance in September 2023 to replace the droplet generator cartridge due to wear and tear, with a documented failure analysis confirming no impact on system performance. (3) Software update in January 2024 to QuantaSoft™ Pro Software v1.7, including installation of the latest patches and compliance enhancements. (4) Annual performance verification in March 2024, confirming droplet uniformity (CV <0.

Packing List

The QX200 AutoDG system is shipped in a custom-designed, double-walled corrugated carton with internal foam padding to ensure protection during transit. The packing list includes: (1) QX200 AutoDG Droplet Digital PCR System main unit, (2) AutoDG droplet generator module, (3) QX200 droplet reader, (4) Power cord (100-240 VAC), (5) USB cable for data transfer, (6) Quick start guide and user manual (digital and printed copies), (7) Installation and calibration certificates, (8) Safety and compliance documentation (CE, FDA, ISO 13485), (9) BIO-RAD ddPCR™ consumables starter kit (10 cartridges, 1 L droplet generation oil, 100 µL ddPCR™ Supermix), (10) Calibration standards and QC materials, (11) Barcode scanner (Honeywell Xenon 1900), (12) 96-well PCR plates (Eppendorf twin.tec®, 10 plates), (13) Pipette tips (0.5-20 µL, 10 racks), (14) Shipping container with desiccant packs and temperature monitoring device. The system is secured to a wooden pallet with stretch wrap for stability.

Additional Information

The BIO-RAD QX200 AutoDG Droplet Digital PCR System is a Class IIa medical device in accordance with EU MDR 2017/746, and is FDA-cleared under the 510(k) premarket notification pathway for in vitro diagnostic use. The system complies with ISO 13485:2016 for quality management systems and ISO 14971:2019 for risk management, ensuring adherence to international standards for medical device safety and performance. The system is CE-marked under the IVD Directive 98/79/EC and carries the UKCA mark for compliance with UK regulations. The QX200 AutoDG system is designed for use in clinical laboratories, research institutions, and biopharmaceutical settings, supporting applications such as gene expression analysis, copy number variation detection, rare mutation screening, and pathogen detection. The system’s droplet digital PCR technology offers unparalleled precision and sensitivity, with a dynamic range of up to 5 logs and a limit of detection (LOD) as low as 0.001%. The system is compatible with a wide range of sample types, including DNA, RNA, and cDNA, and supports both probe-based and EvaGreen® dye-based assays. The AutoDG droplet generator automates the partitioning of samples into approximately 20,000 droplets per well, enabling absolute quantification without the need for standard curves. The system’s fluorescence detection system is optimized for multiplexing, with support for up to two fluorescent channels per well. The system integrates seamlessly with laboratory information systems (LIS) via HL7 or ASTM protocols, enabling automated data transfer and sample tracking. The QuantaSoft™ Pro Software v1.7 includes advanced features such as automated quality control, real-time data visualization, and customizable reporting templates. The system is compatible with a range of third-party reagents and consumables, provided they meet the required specifications for droplet stability and fluorescence compatibility.