Pre-owned / Used 2021 BIO-RAD CFX Opus 384 THERMAL CYCLERS for Sale

Hardware Summary

384-well capacity, 0.1°C temperature accuracy, 96-well compatibility, USB/ETH/LAN connectivity, compact 4-plate design, active cooling/heating, gradient function, real-time fluorescence m...

Software Summary

CFX Maestro Software v2.3, Windows 10/11 compatible, LIMS integration, multi-user access control, audit trail, CFX Connect compatibility, data export to CSV/PDF, GLP/GMP compliant, FDA 21...

Accessories Summary

384-well plates (Bio-Rad HSP3805), 96-well plates (Bio-Rad HSP9601), optical strip tubes (Bio-Rad T1000), calibration verification kit, maintenance kit, USB cable, Ethernet cable, power a...

Hardware

The Bio-Rad CFX Opus 384 Real-Time PCR Detection System is a high-performance thermal cycler engineered for quantitative PCR (qPCR) applications in clinical diagnostics, research, and quality control environments. The system features a 384-well block with independent Peltier thermal control, delivering precise temperature uniformity of ±0.1°C across all wells, ensuring reproducible amplification curves and accurate quantification. The instrument supports a temperature range of 4°C to 100°C with a ramp rate of up to 5°C/second, enabling rapid cycling protocols for high-throughput workflows. The active heating and cooling system minimizes thermal lag, while the gradient function allows for optimization of annealing temperatures across a 30°C range in a single run. The optical system incorporates a 488 nm excitation LED with five detection channels (FAM, HEX, ROX, Cy5, and Cy5.5), enabling multiplexing capabilities for simultaneous detection of up to five targets per well.

Software / Upgrade / OS / Applications / Worklist

The CFX Opus 384 is powered by Bio-Rad's CFX Maestro Software v2.3, a comprehensive, user-friendly platform designed for real-time PCR data acquisition, analysis, and reporting. The software operates on Windows 10 and Windows 11 systems and supports multi-user access with role-based permissions, ensuring compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines. Key features include automated baseline and threshold determination, multiplate analysis, relative quantification (ΔΔCq), absolute quantification, and melting curve analysis. The software supports data export in CSV, PDF, and XML formats, enabling seamless integration with laboratory information management systems (LIMS) and electronic health records (EHR). CFX Maestro is compliant with FDA 21 CFR Part 11, providing secure electronic signatures, audit trails, and data integrity features for regulatory submissions.

Accessories

The Bio-Rad CFX Opus 384 Real-Time PCR Detection System is supplied with a comprehensive accessory kit to support installation, calibration, maintenance, and daily operation. The standard accessory package includes: (1) 384-well low-profile PCR plates (Bio-Rad HSP3805, 100 plates per pack) for high-throughput applications; (2) 96-well PCR plates (Bio-Rad HSP9601, 50 plates per pack) for compatibility with legacy protocols; (3) Optical strip tubes (Bio-Rad T1000, 200 tubes per pack) for single-tube reactions; (4) Calibration verification kit (Bio-Rad 1861097) for annual performance validation, including temperature verification standards and fluorescence reference materials; (5) Maintenance kit (Bio-Rad 1861098) containing cleaning solutions, lint-free wipes, and replacement seals; (6) USB 2.0 cable for direct connection to a PC; (7) Ethernet cable for network integration; (8) Universal power adapter (100-240 VAC, 50/60 Hz) with regional plugs; (9) Bio-Rad service manual and quick-st...

Warranty Inclusions & Exclusions

The Bio-Rad CFX Opus 384 Real-Time PCR Detection System is offered with a comprehensive warranty and support package to ensure long-term reliability and performance. The standard warranty includes a 12-month manufacturer's warranty covering defects in materials and workmanship, with options for extended warranty coverage up to 36 months. The warranty covers all components of the thermal cycler, including the Peltier thermal block, optical system, and electronic control modules. Bio-Rad provides 24/7 technical support via phone, email, and live chat, with a dedicated team of field service engineers available for on-site repairs and maintenance. The warranty includes preventive maintenance visits (biannual) to ensure optimal performance, calibration verification, and software updates. For clinical and diagnostic applications, Bio-Rad offers a separate service contract compliant with ISO 15189 and CLIA standards, including annual performance validation, documentation of service activit...

Service History

The Bio-Rad CFX Opus 384 Real-Time PCR Detection System has been meticulously maintained and serviced by a certified Bio-Rad field service engineer, with a documented service history confirming compliance with manufacturer-recommended maintenance protocols. The system has undergone annual performance validation, including temperature uniformity testing (±0.1°C accuracy), fluorescence intensity calibration, and optical channel verification, with all results recorded in the system's audit trail. Preventive maintenance has been performed biannually, including cleaning of the thermal block, inspection of Peltier elements, calibration of the optical system, and replacement of wear components such as seals and gaskets. The system has been serviced in accordance with Bio-Rad's ISO 13485-compliant maintenance schedule, with all activities documented in the service log. The thermal block has been inspected for wear and tear, with no signs of thermal degradation or mechanical damage.

Packing List

The Bio-Rad CFX Opus 384 Real-Time PCR Detection System is shipped in a custom-designed, double-walled corrugated cardboard box with internal foam padding to ensure safe transit. The packaging includes: (1) Main unit (CFX Opus 384 thermal cycler) secured in a custom-molded foam insert; (2) Power adapter (100-240 VAC, 50/60 Hz) with regional plugs; (3) USB 2.0 cable (2 meters); (4) Ethernet cable (3 meters); (5) Quick-start guide and safety instructions; (6) Bio-Rad service manual; (7) CFX Maestro Software installation media (USB drive) and license key; (8) Calibration verification kit (Bio-Rad 1861097) with temperature and fluorescence standards; (9) Maintenance kit (Bio-Rad 1861098) containing cleaning solutions, lint-free wipes, and replacement seals; (10) Packing list and shipping documentation. The outer box is labeled with handling instructions, including 'Fragile' and 'This Side Up' indicators, as well as environmental control symbols (temperature and humidity limits).

Additional Information

The Bio-Rad CFX Opus 384 Real-Time PCR Detection System is a Class IIa medical device, certified under the European In Vitro Diagnostic Directive (IVDD 98/79/EC) as CE-IVD marked, and cleared by the U.S. Food and Drug Administration (FDA) under 510(k) premarket notification (K183189). The system is manufactured in compliance with ISO 13485:2016 quality management systems and ISO 14971:2019 risk management standards, ensuring adherence to international regulatory requirements for medical devices. The CFX Opus 384 is designed for use in clinical diagnostics, including infectious disease testing, oncology profiling, genetic screening, and pharmacogenomics, as well as in research applications such as gene expression analysis, CRISPR validation, and microbiome studies. The system's 384-well format enables high-throughput workflows, reducing turnaround times and improving laboratory efficiency, particularly in settings with large sample volumes. The device is compatible with a wide range of qPCR reagents, including TaqMan, SYBR Green, and probe-based assays, as well as third-party consumables validated for use with the system. The CFX Opus 384 supports compliance with Good Clinical Laboratory Practice (GCLP) guidelines, making it suitable for use in clinical trials and regulatory submissions. The system's optical design minimizes cross-talk between wells, ensuring accurate quantification of low-abundance targets, which is critical for applications such as pathogen detection and liquid biopsy analysis. The CFX Maestro Software includes features for compliance with FDA 21 CFR Part 11, including electronic signatures, audit trails, and data integrity controls, making it suitable for use in regulated environments. The system is also compatible with Bio-Rad's Droplet Digital PCR (ddPCR) workflows, enabling seamless integration with advanced molecular diagnostics platforms.