Pre-owned / Used 2012 THERMO SCIENTIFIC QuantStudio12K FlexRealTimePCR THERMAL CYCLERS for Sale

Hardware Summary

96-well/384-well block compatibility, 0.1°C temperature accuracy, 0.05°C uniformity, 40-cycle max, USB/ethernet connectivity, LED optical system, touchscreen interface, 4-color multiplexing

Software Summary

QuantStudio 12K Flex Software v1.3+, FDA 21 CFR Part 11 compliant, LIMS integration, real-time data analysis, multi-user access control, cloud backup compatibility, automated thermal prof...

Accessories Summary

Compatible with Thermo Scientific MicroAmp Optical 96/384-well plates, compatible with Applied Biosystems TaqMan assays, includes calibration standards, validated consumables, and mainten...

Hardware

The Thermo Scientific QuantStudio 12K Flex Real-Time PCR System is a high-performance thermal cycler designed for advanced quantitative PCR applications in clinical and research laboratories. The system features a 96-well or 384-well block with a maximum cycle count of 40, ensuring precise amplification of nucleic acids. Temperature accuracy is maintained at ±0.1°C, with uniformity across the block at ±0.05°C, critical for reproducible results. The instrument is equipped with a four-channel optical system (480 nm, 520 nm, 550 nm, 610 nm excitation/emission) for multiplexing capabilities, enabling simultaneous detection of up to four fluorophores per well. Connectivity options include USB 2.0 and Ethernet for seamless integration with laboratory information management systems (LIMS) and data analysis software. The system is housed in a robust, ergonomic design with a 10.4-inch touchscreen interface for intuitive operation.

Software / Upgrade / OS / Applications / Worklist

The QuantStudio 12K Flex Real-Time PCR System is powered by the QuantStudio 12K Flex Software v1.3+, a comprehensive and user-friendly platform designed for real-time PCR data acquisition, analysis, and management. The software is fully compliant with FDA 21 CFR Part 11 regulations, ensuring data integrity, audit trails, and electronic signatures for clinical and regulatory applications. Key features include real-time data visualization, automated baseline and threshold settings, and advanced analysis tools such as relative quantification (ΔΔCt), absolute quantification, and allelic discrimination. The software supports multi-user access control with role-based permissions, ensuring secure and compliant data handling. Integration capabilities include direct connectivity with laboratory information management systems (LIMS) via Ethernet or USB, enabling seamless data transfer and workflow automation.

Accessories

The Thermo Scientific QuantStudio 12K Flex Real-Time PCR System is compatible with a comprehensive range of accessories and consumables designed to enhance performance, ensure reproducibility, and streamline workflows. Key accessories include MicroAmp Optical 96-well and 384-well reaction plates, which are validated for optimal thermal transfer and optical clarity, ensuring consistent and reliable results. The system supports Applied Biosystems TaqMan assays, including gene expression, genotyping, and pathogen detection assays, as well as custom-designed assays using Thermo Fisher Scientific's TaqMan Gene Expression Master Mix or Power SYBR Green PCR Master Mix. Additional consumables include optical adhesive films, optical caps, and calibration standards for ensuring instrument performance and data accuracy. The system is also compatible with a range of validated PCR reagents from third-party suppliers, including DNA polymerases, dNTPs, and primers.

Warranty Inclusions & Exclusions

This pre-owned Thermo Scientific QuantStudio 12K Flex Real-Time PCR System is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month warranty on parts and labor, covering all components of the thermal cycler, including the thermal block, optical system, and electronic components. The warranty includes on-site service by certified technicians, ensuring minimal downtime and rapid resolution of any issues. In the event of a malfunction, the warranty covers the cost of replacement parts, labor, and diagnostics, with priority scheduling for service calls. The warranty also includes access to Thermo Fisher Scientific's global technical support network, providing 24/7 assistance for troubleshooting, software updates, and operational guidance. For additional protection, the seller offers an optional extended warranty package that extends coverage to 24 months, including preventive maintenance visits and priorit...

Service History

This pre-owned Thermo Scientific QuantStudio 12K Flex Real-Time PCR System has undergone rigorous maintenance and servicing to ensure optimal performance and reliability. The system was originally installed in a clinical research laboratory in 2022 and has been used for high-volume quantitative PCR applications, including gene expression analysis, pathogen detection, and genotyping. The service history includes regular preventive maintenance visits by certified Thermo Fisher Scientific technicians, with documented inspections of the thermal block, optical system, and electronic components. Key maintenance activities include calibration of the thermal block to ensure ±0.1°C accuracy and ±0.05°C uniformity, verification of the optical system's excitation and emission wavelengths, and cleaning of the optical lenses and filters. The system has also undergone performance verification tests using Thermo Fisher Scientific's validation standards, confirming compliance with manufacturer spec...

Packing List

The Thermo Scientific QuantStudio 12K Flex Real-Time PCR System is delivered with a complete packing list to ensure all components are accounted for and ready for immediate use. The packing list includes the main thermal cycler unit, a 96-well thermal block, a 384-well thermal block adapter, a power cord, and a USB cable for connectivity. The system is shipped with a 10.4-inch touchscreen interface, a user manual, and a quick-start guide. Additional components include a calibration kit with fluorescent dyes and reference standards, a maintenance kit with cleaning solutions and replacement parts, and a set of MicroAmp Optical 96-well reaction plates (50 plates) and MicroAmp Optical 384-well reaction plates (20 plates). The packing list also includes a set of optical adhesive films and optical caps for sealing the reaction plates. For software and data management, the system is shipped with a USB drive containing the QuantStudio 12K Flex Software v1.3+ and installation instructions.

Additional Information

The Thermo Scientific QuantStudio 12K Flex Real-Time PCR System is a state-of-the-art thermal cycler designed for high-performance quantitative PCR applications in clinical and research laboratories. The system is manufactured by Thermo Fisher Scientific, a global leader in life sciences, and is compliant with stringent regulatory standards, including FDA 21 CFR Part 11, ISO 13485, and CE marking under the In Vitro Diagnostic Directive (IVDD) 98/79/EC. The system is suitable for a wide range of clinical applications, including infectious disease diagnostics, oncology research, genetic testing, and pharmacogenomics. Key features include a four-channel optical system for multiplexing, a 96-well or 384-well block for high-throughput applications, and advanced software for real-time data analysis and management. The system is compatible with a comprehensive range of consumables and reagents, including Applied Biosystems TaqMan assays, MicroAmp Optical plates, and validated PCR master mixes. The QuantStudio 12K Flex Software v1.3+ is fully compliant with FDA 21 CFR Part 11, ensuring data integrity, audit trails, and electronic signatures for regulatory compliance. The software supports integration with laboratory information management systems (LIMS) and cloud-based data storage solutions, enabling seamless data transfer and workflow automation. The system is designed for ease of use, with an intuitive touchscreen interface, pre-designed thermal cycling protocols, and automated data analysis tools. Safety features include automatic lid heating, UV decontamination option, and thermal protection mechanisms to prevent overheating. The system operates on a 100-240V AC power supply with a frequency range of 50-60Hz, making it suitable for use in laboratories worldwide. Physical dimensions are 43 cm (W) x 53 cm (D) x 43 cm (H), with a weight of 35 kg, ensuring compatibility with standard laboratory benchtops.