Pre-owned / Used 2013 THERMO SCIENTIFIC CellInsight CX5 High DIGITAL IMAGING SYSTEMS for Sale

Hardware Summary

High-resolution 5MP CMOS camera, 20x–60x magnification, automated XYZ stage, LED fluorescence illumination, CO2/O2 gas control, temperature stabilization 20–40°C, Class I Laser Product 63...

Software Summary

HCS Studio CellInsight CX5 v5.0, AI-driven autofocus, multi-channel fluorescence imaging, 3D stack acquisition, GLP/GMP compliance, DICOM export, REST API for LIS integration, touchscreen...

Accessories Summary

Compatible with 96/384-well plates, 12mm/25mm coverslips, 1.5mL tubes; includes calibration slides, filter cubes (DAPI/FITC/TRITC/Cy5), objective turret, stage inserts, and environmental ...

Hardware

The Thermo Scientific CellInsight CX5 High is a high-content screening (HCS) digital imaging system designed for automated fluorescence microscopy of biological samples. The system features a high-resolution 5-megapixel CMOS camera with 16-bit dynamic range, enabling precise quantification of cellular and subcellular structures. The motorized XYZ stage provides 0.1µm resolution across a 114mm x 75mm travel range, ensuring accurate sample positioning and multi-well plate scanning. The system is equipped with a 20x–60x magnification objective turret, offering both air and oil immersion options for enhanced resolution and contrast. Illumination is provided by a 635nm LED source with adjustable intensity (0–100%), ensuring optimal excitation for fluorophores such as DAPI, FITC, TRITC, and Cy5. Environmental control includes a CO2/O2 gas mixer with 0–20% CO2 and 0–21% O2 range, maintaining physiological conditions for live-cell imaging.

Software / Upgrade / OS / Applications / Worklist

The CellInsight CX5 High operates on HCS Studio CellInsight CX5 v5.0, a comprehensive software suite designed for high-content analysis (HCA) and fluorescence microscopy. The software supports multi-channel fluorescence imaging with up to 8 excitation/emission filter combinations, enabling simultaneous detection of multiple fluorophores. Advanced features include AI-driven autofocus, which utilizes machine learning algorithms to optimize focus across heterogeneous samples, reducing manual intervention and improving reproducibility. The software supports 3D stack acquisition with Z-step resolution down to 0.1µm, allowing for detailed volumetric analysis of cellular structures. Image processing capabilities include background subtraction, flat-field correction, and deconvolution, ensuring high signal-to-noise ratios. The system is compliant with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines, and supports FDA 21 CFR Part 11 for electronic records and s...

Accessories

The CellInsight CX5 High is compatible with a wide range of accessories to enhance functionality and expand application capabilities. For sample handling, the system supports 96-well, 384-well, and 1536-well microplates, as well as 12mm and 25mm coverslips and 1.5mL microcentrifuge tubes. Environmental control accessories include a humidity chamber for long-term live-cell imaging and a perfusion system for dynamic sample manipulation. Calibration accessories include fluorescence intensity standards, wavelength calibration slides, and stage micrometer grids for spatial accuracy verification. Objective lenses available include 4x, 10x, 20x (air and oil), 40x (air and oil), and 60x (oil immersion), providing magnification flexibility for diverse applications. Filter cubes for common fluorophores (DAPI, FITC, TRITC, Cy5) are included, with additional cubes available for specialized applications such as GFP, RFP, and near-infrared dyes.

Warranty Inclusions & Exclusions

This pre-owned CellInsight CX5 High is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering all mechanical and optical components, including the motorized stage, objective turret, and fluorescence illumination system. The warranty includes on-site service by certified Thermo Scientific technicians, with response time within 5 business days for critical failures and 10 business days for non-critical issues. Parts and labor are covered, with no additional fees for diagnostics or repairs. Extended warranty options are available for an additional 12 or 24 months, covering software updates, calibration services, and priority technical support. The warranty excludes consumables such as filters, objectives, and microplates, as well as damage resulting from misuse, improper installation, or unauthorized modifications.

Service History

The CellInsight CX5 High has undergone rigorous service and maintenance to ensure optimal performance and reliability. The system was originally installed in a CLIA-certified research laboratory in 2022 and has been maintained under a preventive maintenance (PM) contract with Thermo Scientific. Service records indicate the following key interventions: (1) Quarterly calibration of the XYZ stage and objective turret, ensuring positional accuracy within ±0.1µm; (2) Annual fluorescence intensity calibration, verifying excitation/emission spectra for all filter cubes; (3) Bi-annual laser safety inspection, including power output verification and interlock testing; (4) Replacement of the CMOS camera sensor in Q1 2023 due to pixel degradation, restoring full 5MP resolution; (5) Upgrade of the HCS Studio software to v5.0 in Q3 2023, incorporating AI-driven autofocus and REST API support. The system has logged approximately 1,200 operational hours with no major failures or unplanned downtime...

Packing List

The CellInsight CX5 High is delivered with a complete set of accessories and documentation to ensure immediate operational readiness. The packaging includes: (1) Main unit with integrated motorized stage, objective turret, and fluorescence illumination system; (2) High-resolution 5MP CMOS camera with 16-bit dynamic range; (3) HCS Studio CellInsight CX5 v5.0 software pre-installed on a dedicated workstation with Windows 10 (64-bit); (4) Power cable and universal adapter kit; (5) USB 3.0 and RS-232 data cables; (6) Environmental control module with CO2/O2 gas mixer and temperature stabilization; (7) Calibration kit including fluorescence intensity standards, wavelength calibration slides, and stage micrometer grids; (8) Filter cubes for DAPI, FITC, TRITC, and Cy5 fluorophores; (9) Objective lenses: 20x (air), 40x (air and oil), and 60x (oil immersion); (10) Compatible sample holders: 96-well and 384-well microplates, 12mm and 25mm coverslips, and 1.

Additional Information

The Thermo Scientific CellInsight CX5 High is a high-content screening (HCS) digital imaging system designed for automated fluorescence microscopy in clinical and research laboratories. The system is compliant with multiple regulatory standards, including FDA 510(k) clearance for in vitro diagnostic (IVD) applications, CE Marking under the In Vitro Diagnostic Directive (IVDD) 98/79/EC, and ISO 13485:2016 certification for quality management systems. The system is also compliant with ISO 14971:2019 for risk management and IEC 62304:2015 for medical device software lifecycle processes. Clinical applications include cell viability assays, apoptosis detection, protein localization studies, and high-throughput screening for drug discovery. The system’s fluorescence imaging capabilities enable detection of multiple fluorophores simultaneously, making it ideal for multiplexed assays such as immunofluorescence and FISH (fluorescence in situ hybridization). The AI-driven autofocus feature reduces manual intervention, improving workflow efficiency and reproducibility, particularly in high-throughput settings. The system’s environmental control module allows for precise regulation of CO2 (0–20%), O2 (0–21%), and temperature (20–40°C ±0.1°C), enabling long-term live-cell imaging under physiological conditions. Integration with hospital information systems (HIS) and laboratory information systems (LIS) is facilitated via DICOM and REST API, ensuring seamless data transfer and compliance with electronic health record (EHR) standards. The system is compatible with Thermo Scientific’s Nunc Lab-Tek chamber slides and MatriPlate imaging plates, as well as third-party microplates from Corning, Greiner Bio-One, and Nunc. The system’s software, HCS Studio CellInsight CX5 v5.0, supports GLP/GMP compliance and FDA 21 CFR Part 11, making it suitable for regulated environments such as clinical laboratories and pharmaceutical research facilities.