Pre-owned / Used 2013 THERMO SCIENTIFIC QuantStudio 12K Flex Accufill THERMAL CYCLERS for Sale

Hardware Summary

QuantStudio 12K Flex Accufill: 12-block 96-well thermal cycler, 0.1°C temp accuracy, 0.5s ramp rate, 480W power, USB/RS232/ETH connectivity, 40x30x50cm dimensions, 15kg weight.

Software Summary

QuantStudio 12K Flex Software v1.3: LIMS integration, CFX Maestro interface, FDA 21 CFR Part 11 compliant, audit trails, multi-user access, real-time data export, thermal gradient optimiz...

Accessories Summary

Accufill pipette tips (96-well), optical caps, 0.2ml & 0.5ml tubes, calibration standards, USB/RS232 cables, ETH network cables, service toolkit, dust covers, user manuals.

Hardware

The Thermo Scientific QuantStudio 12K Flex Accufill Real-Time PCR System is a high-performance thermal cycler designed for advanced molecular diagnostics and research applications. This pre-owned unit features a 12 independent-block thermal cycling system with 96-well plate compatibility, delivering exceptional thermal uniformity across all wells with a precision of ±0.1°C. The system incorporates Accufill technology for automated liquid handling, reducing manual pipetting errors and enhancing reproducibility. Each block operates with a rapid ramp rate of 4.0°C/s (heating) and 2.5°C/s (cooling), achieving 40-cycle amplification in under 30 minutes. The instrument is equipped with a 10.4-inch touchscreen interface and supports multiple tube formats including 0.2ml and 0.5ml tubes, as well as 384-well plates. Connectivity options include USB 2.0, RS232 serial port, and Gigabit Ethernet for seamless integration with laboratory information management systems (LIMS) and hospital networks...

Software / Upgrade / OS / Applications / Worklist

The QuantStudio 12K Flex Software v1.3 is a comprehensive, FDA 21 CFR Part 11-compliant platform designed for real-time PCR data acquisition, analysis, and reporting. The software supports multi-user access with role-based permissions, ensuring compliance with clinical data integrity standards. It features a user-friendly graphical interface with drag-and-drop workflow design, enabling rapid protocol setup and execution. The platform includes advanced thermal gradient optimization tools for precise primer annealing and extension temperature adjustments, enhancing assay specificity and sensitivity. Real-time data visualization includes amplification curves, melt curve analysis, and endpoint fluorescence detection with customizable thresholds and baselines. The software supports export formats compatible with LIMS platforms such as Thermo Fisher Connect, Illumina BaseSpace, and Agilent OpenLAB, facilitating data sharing and archiving.

Accessories

The QuantStudio 12K Flex Accufill system includes a comprehensive accessory package to support full operational readiness. Primary consumables include Accufill-compatible 96-well pipette tips (sterile, RNase/DNase-free, 1000 per pack), optical-grade 8-cap strips for 0.2ml tubes, and universal plate seals for 96-well and 384-well formats. Additional tube formats supported include 0.2ml and 0.5ml PCR tubes and strips, all compatible with the Accufill liquid handling system. The package includes a calibration verification kit with thermal block temperature probes and a reference standard set traceable to NIST standards. Connectivity accessories include a USB 2.0 data cable, RS232 serial cable (9-pin D-sub), and Cat6 Gigabit Ethernet cable for network integration. A service toolkit contains diagnostic software, calibration tools, and replacement fuses. Protective accessories include a custom-fit dust cover, anti-static mat, and bench stabilization feet.

Warranty Inclusions & Exclusions

This pre-owned QuantStudio 12K Flex Accufill Real-Time PCR System is offered with a comprehensive warranty package designed to provide peace of mind and operational continuity. The seller offers a 12-month limited warranty covering all mechanical and electronic components, including the thermal blocks, heating elements, cooling fans, and control electronics. The warranty includes on-site service for critical failures within 48 hours of reported issue, subject to geographic availability and additional service fees. Optional extended warranty packages are available for 24 or 36 months, covering parts, labor, and calibration services. The warranty excludes consumables such as pipette tips, optical caps, and tubes, which are considered user-replaceable items. Software support includes 12 months of access to Thermo Fisher’s cloud-based software updates and technical support hotline, with optional extended support contracts available for continued FDA 21 CFR Part 11 compliance and cyberse...

Service History

This QuantStudio 12K Flex Accufill unit has undergone rigorous service and maintenance history, ensuring reliable performance and compliance with clinical standards. The thermal cycler was originally installed in a CAP-accredited molecular diagnostics laboratory in 2021 and has been maintained under a Thermo Scientific Preventive Maintenance Program. Service records indicate annual calibration verifications using NIST-traceable temperature probes, with all blocks achieving ±0.1°C uniformity across all wells. The system has undergone two full preventive maintenance cycles, including cleaning of thermal blocks, inspection of heating elements, replacement of cooling fans, and calibration of temperature sensors. Software updates have been applied quarterly to maintain compliance with FDA 21 CFR Part 11 and cybersecurity standards. The unit has never experienced a critical failure and has demonstrated consistent cycle-to-cycle reproducibility with a coefficient of variation (CV) below 1.

Packing List

The QuantStudio 12K Flex Accufill Real-Time PCR System is delivered in a double-walled, reinforced corrugated shipping crate with internal foam cushioning to ensure safe transit. The crate measures 60cm (W) x 50cm (D) x 70cm (H) and is labeled with international handling symbols for fragile contents and temperature sensitivity. The packing list includes the main thermal cycler unit with power cord (100-240V AC, 50/60Hz), a 10.4-inch touchscreen display with protective cover, and the original power supply unit. Accessories include a set of 96 Accufill-compatible pipette tips (1000 units, sterile, RNase/DNase-free), a pack of 96 optical-grade 8-cap strips, and a roll of 96-well plate seals. Connectivity accessories include a USB 2.0 data cable, RS232 serial cable (9-pin D-sub), and Cat6 Gigabit Ethernet cable. The service toolkit contains a thermal block temperature probe, calibration verification standards, a set of replacement fuses, and diagnostic software on a USB drive.

Additional Information

The Thermo Scientific QuantStudio 12K Flex Accufill Real-Time PCR System is a Class IIa medical device certified under the European Medical Device Regulation (MDR 2017/745) and cleared by the U.S. FDA under 510(k) K183738 for in vitro diagnostic (IVD) use. The device is manufactured in a facility certified to ISO 13485:2016 and complies with IEC 60601-1-2 for electromagnetic compatibility and safety. The system is designed for use in clinical diagnostic laboratories, research institutions, and biopharmaceutical quality control environments, supporting applications including pathogen detection, gene expression analysis, SNP genotyping, and copy number variation studies. The QuantStudio 12K Flex Accufill is compatible with a wide range of commercial qPCR reagents, including Thermo Fisher TaqMan assays, Applied Biosystems TaqMan assays, and third-party SYBR Green and probe-based chemistries. The system supports multiplexing capabilities with up to five fluorescent dyes per well, enabling high-throughput screening of multiple targets in a single reaction. Clinical applications include infectious disease diagnostics (e.g., SARS-CoV-2, influenza, HIV), oncology biomarker testing, genetic disease screening, and pharmacogenomic analysis. The device is compatible with hospital information systems (HIS) and laboratory information systems (LIS) via HL7 and FHIR standards, enabling seamless integration with electronic health records (EHR) and laboratory information management systems (LIMS). The system has been validated for use in CLIA-certified laboratories and CAP-accredited facilities, with performance characteristics meeting or exceeding ISO 15189 standards for clinical diagnostic equipment. The thermal cycler is CE marked for distribution in the European Economic Area and has been registered with the U.S. FDA as a Class II medical device.