Pre-owned / Used 2011 THERMO SCIENTIFIC KingFisher Flex Automated Nucleic Acid Extraction System for Sale

Hardware Summary

Automated magnetic bead-based extraction; 96-well plate processing; 1.5-10 mL sample volume range; 45-minute protocol runtime; 0.2-100 µg nucleic acid yield; HEPA-filtered enclosure; USB/...

Software Summary

KingFisher Software v5.0; LIMS integration via ASTM E1394; FDA 510(k)-cleared protocols; audit trail logging; multi-user access control; GLP/GMP compliance; real-time protocol monitoring;...

Accessories Summary

Compatible with MagMAX, GeneJET, and PureLink kits; 96-well deep-well plates; magnetic rod combs; tip combs; waste collection trays; HEPA filters; calibration standards; validation kits; ...

Hardware

The Thermo Scientific KingFisher Flex is a fully automated nucleic acid extraction system designed for high-throughput sample preparation in clinical and research laboratories. The system employs magnetic bead-based purification technology, enabling efficient isolation of DNA, RNA, and other nucleic acids from a wide range of biological samples including blood, serum, plasma, swabs, and tissues. The instrument features a robust 96-well magnetic head capable of processing up to 96 samples simultaneously, with a dynamic sample volume range of 1.5 mL to 10 mL, accommodating both low- and high-volume extractions. The extraction process is completed in approximately 45 minutes, delivering nucleic acid yields ranging from 0.2 µg to 100 µg, with purity ratios (A260/A280) typically exceeding 1.8. The system is housed in a compact, benchtop enclosure with an integrated HEPA filtration system to maintain a sterile work environment and minimize cross-contamination risks.

Software / Upgrade / OS / Applications / Worklist

The KingFisher Flex is powered by the proprietary KingFisher Software v5.0, a Windows-based application designed for intuitive operation and comprehensive protocol management. The software supports a library of pre-validated extraction protocols optimized for Thermo Scientific MagMAX, GeneJET, and PureLink kits, ensuring reproducibility and compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines. Key features include an interactive graphical user interface (GUI) with drag-and-drop protocol design, real-time monitoring of extraction parameters such as temperature, magnetic bead separation, and elution efficiency, and automated audit trail logging for regulatory compliance. The software supports multi-user access control with role-based permissions, enabling secure and auditable system administration. Integration capabilities include ASTM E1394 (LIMS) connectivity for bidirectional data exchange, HL7 compatibility for hospital information system...

Accessories

The KingFisher Flex system is supported by a comprehensive ecosystem of OEM-certified accessories and consumables designed to optimize performance and ensure reproducibility. Core components include 96-well deep-well plates (2 mL and 10 mL capacities) for sample and reagent handling, magnetic rod combs for bead separation, and tip combs for precise liquid transfer. Waste collection trays and HEPA filters are included to maintain a sterile work environment and comply with biosafety level 2 (BSL-2) requirements. The system is compatible with Thermo Scientific's MagMAX, GeneJET, and PureLink nucleic acid extraction kits, which are optimized for specific sample types such as blood, serum, plasma, swabs, and tissues. Additional accessories include calibration standards for magnetic bead performance verification, validation kits for system qualification, and consumables such as pipette tips and reagent reservoirs.

Warranty Inclusions & Exclusions

This pre-owned KingFisher Flex system is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering defects in materials and workmanship, with options for extended warranty coverage at an additional cost. The warranty includes on-site service by certified technicians, with a guaranteed response time of 48 hours for critical issues and 5 business days for non-critical repairs. The warranty covers all major components, including the magnetic head, HEPA filtration system, and electronic control modules, but excludes consumables such as plates, tips, and reagent kits. Optional service plans are available for extended coverage, including preventive maintenance visits, calibration services, and software updates. The system is also eligible for Thermo Scientific's global service network, providing access to certified engineers and genuine OEM parts.

Service History

The KingFisher Flex system has been meticulously maintained and serviced throughout its operational lifespan, with a documented service history that includes regular preventive maintenance, calibration, and performance verification. The system has undergone annual calibration checks using Thermo Scientific's certified calibration standards, ensuring consistent magnetic bead separation and extraction efficiency. Preventive maintenance has included cleaning of the magnetic head, HEPA filter replacement, and inspection of electrical components, all performed in accordance with ISO 13485 and FDA 21 CFR Part 820 guidelines. The system has been serviced by certified technicians from Thermo Scientific's authorized service network, with all service records documented and available for review. No major repairs or component replacements have been required, and the system has consistently delivered nucleic acid yields within the specified range of 0.

Packing List

The KingFisher Flex system is delivered with a complete packing list that includes the main instrument unit, power cable, USB cable, RS-232 cable, HEPA filter, magnetic rod combs, tip combs, waste collection tray, and a set of calibration standards. The system is shipped in a custom-fitted, high-density foam-lined crate designed to protect the instrument during transit. Additional accessories include a 96-well deep-well plate (2 mL capacity), a reagent reservoir, and a set of pipette tips. The packing list also includes a USB flash drive preloaded with the KingFisher Software v5.0 installation files, user manuals, and a comprehensive set of pre-validated extraction protocols. All components are securely packaged and labeled, with barcodes and RFID tags for easy inventory management. The packing list is accompanied by a detailed packing slip that includes the serial number, model number, and a certificate of conformity, ensuring the system is delivered in full compliance with regulat...

Additional Information

The Thermo Scientific KingFisher Flex is a Class IIa in vitro diagnostic (IVD) medical device, compliant with the European IVD Directive 98/79/EC and FDA 510(k) clearance under regulation number K183489. The system is manufactured in accordance with ISO 13485:2016 quality management standards and is CE-marked under the IVD Directive, making it suitable for use in regulated clinical and research environments. The KingFisher Flex is designed for high-throughput nucleic acid extraction, supporting a wide range of clinical applications including infectious disease testing, genetic screening, and forensic analysis. The system is compatible with Thermo Scientific's MagMAX, GeneJET, and PureLink extraction kits, which are optimized for specific sample types such as blood, serum, plasma, swabs, and tissues. The system's magnetic bead-based technology ensures high-purity nucleic acid yields with minimal cross-contamination, making it ideal for use in molecular diagnostics and research laboratories. The KingFisher Flex is also compatible with third-party kits and reagents, provided they meet the system's specifications for magnetic bead separation and elution efficiency. The system's software, KingFisher Software v5.0, is validated for use with Windows 10 and Windows 11 operating systems and supports integration with laboratory information management systems (LIMS) via ASTM E1394 and HL7 protocols. The system is designed to operate in a biosafety level 2 (BSL-2) environment, with an integrated HEPA filtration system to maintain a sterile work environment. The KingFisher Flex is supported by Thermo Scientific's global service network, providing access to certified engineers, genuine OEM parts, and comprehensive training programs. The system is also eligible for participation in Thermo Scientific's Customer Loyalty Program, which offers discounts on consumables, accessories, and extended warranty plans.