Pre-owned / Used 2010 GE HEALTHCARE ÄKTApilot FPLC System LIQUID CHROMATOGRAPHY SYSTEMS for Sale

Hardware Summary

ÄKTApilot FPLC system with 0.001–100 mL/min flow range, 1–2800 psi pressure, P-960 pump, UV-900 detector, 280–700 nm wavelength, 0.75–2.0 mL/min precision, stainless steel flow path, 21 C...

Software Summary

UNICORN 7.0 control software with automated method development, real-time monitoring, data export to LIMS, audit trail, user access control, GLP/GMP compliance, multi-user profiles, remot...

Accessories Summary

Compatible with GE Healthcare Superloop 100 mL, Frac-950 fraction collector, pH/C conductivity monitor, AKTAprime buffer kit, GE Healthcare prepacked columns (HiPrep, Sepharose, Capto), 2...

Hardware

The ÄKTApilot FPLC system is a benchtop liquid chromatography platform engineered for high-precision protein purification, biomolecule separation, and analytical-scale chromatography in clinical and research laboratories. The system features a dual-piston P-960 pump delivering flow rates from 0.001 to 100 mL/min with ±0.5% accuracy and pressure stability up to 2800 psi, ensuring reproducible separations across diverse applications. The integrated UV-900 detector offers dual-wavelength monitoring (280–700 nm) with 0.001–2.0 AUFS sensitivity and 0.1–2.0 mL/min flow cell compatibility, enabling accurate quantification of nucleic acids, proteins, and small molecules. The system includes a stainless steel flow path with chemically resistant PEEK components, minimizing sample carryover and ensuring compatibility with organic solvents, acids, and bases. The ÄKTApilot supports multiple column formats, including GE Healthcare prepacked columns (HiPrep, Sepharose, Capto) and custom-packed col...

Software / Upgrade / OS / Applications / Worklist

The ÄKTApilot FPLC system operates on UNICORN 7.0 software, a comprehensive chromatography control and data analysis platform designed for seamless integration with laboratory information management systems (LIMS) and electronic health records (EHR). UNICORN 7.0 provides automated method development tools, including gradient scouting, buffer blending, and peak tracking, enabling users to optimize purification protocols with minimal manual intervention. The software supports real-time monitoring of all system parameters, including pressure, flow rate, UV absorbance, conductivity, and pH, with customizable alarm thresholds for critical events such as column fouling or buffer depletion. Data acquisition occurs at up to 100 Hz, ensuring high-resolution chromatograms for accurate peak integration and quantification. UNICORN 7.0 includes advanced features such as multi-user profiles with role-based access control, audit trails compliant with 21 CFR Part 11 and EU Annex 11, and electronic ...

Accessories

The ÄKTApilot FPLC system is compatible with a comprehensive range of accessories designed to enhance functionality, expand application capabilities, and streamline workflows. Key accessories include the Superloop 100 mL for sample loading, the Frac-950 fraction collector with 100-tube capacity and 0.5–50 mL collection volume range, and the pH/C conductivity monitor for real-time monitoring of buffer conditions. The system supports GE Healthcare prepacked columns, including HiPrep 16/60 Sephacryl S-200 HR for protein purification, HiPrep 26/10 Desalting for buffer exchange, and Capto Q for ion exchange chromatography. Additional column options include Superdex 200 Increase 10/300 GL for high-resolution size exclusion chromatography and Mono Q 5/50 GL for protein separation. The system is compatible with 2.1 mm ID PEEK tubing for low-dead-volume connections, reducing sample dilution and improving peak resolution.

Warranty Inclusions & Exclusions

The ÄKTApilot FPLC system is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The standard warranty includes a 12-month parts and labor coverage from the date of installation, covering defects in materials and workmanship under normal use conditions. The warranty covers all system components, including the P-960 pump, UV-900 detector, touchscreen interface, and integrated electronics, with the exception of consumables such as columns, tubing, and buffers. The warranty also includes access to GE Healthcare’s global service network, with priority response times for technical support and on-site service within 48 hours for critical issues. Extended warranty options are available, including 24-month and 36-month plans, which provide additional coverage for wear-and-tear components and priority access to software updates and firmware upgrades. The warranty is transferable to new owners, ensuring continuity of coverage.

Service History

The ÄKTApilot FPLC system has been meticulously maintained with a documented service history, ensuring optimal performance and reliability. The system was installed in a controlled laboratory environment with a dedicated power supply and environmental controls, including temperature and humidity monitoring. The initial installation included an on-site commissioning by a GE Healthcare-certified engineer, with verification of all system parameters, including flow rate accuracy (±0.5%), pressure stability (≤2800 psi), and UV detector linearity (R² ≥ 0.999). The system has undergone regular preventive maintenance, including quarterly inspections of the pump seals, detector lamps, and flow path components, with replacement of consumables such as tubing and fittings as needed. The UV-900 detector has been calibrated annually using NIST-traceable standards, with verification of wavelength accuracy (±1 nm) and absorbance linearity (0.001–2.0 AUFS).

Packing List

The ÄKTApilot FPLC system is supplied with a complete packing list to ensure a smooth installation and setup process. The shipment includes the main system unit with integrated P-960 pump, UV-900 detector, and touchscreen interface, along with a power cable, Ethernet cable, and USB cable for connectivity. The packing list also includes the Superloop 100 mL for sample loading, a set of 2.1 mm ID PEEK tubing with connectors, and a toolkit for routine maintenance. Additional accessories include a buffer kit with pre-configured lines, a waste bottle with level sensor, and a set of spare fuses. The shipment also includes the UNICORN 7.0 software installation media, a user manual, and a quick-start guide. The packing list is accompanied by a detailed packing slip with serial numbers for all components, ensuring traceability and compliance with regulatory requirements. The system is shipped in a double-walled cardboard box with foam padding to protect against transit damage, and all compon...

Additional Information

The ÄKTApilot FPLC system is a versatile liquid chromatography platform designed for high-precision biomolecule separation and purification in clinical, research, and pharmaceutical laboratories. The system is manufactured by GE Healthcare, a global leader in life sciences instrumentation, and is compliant with stringent regulatory standards, including CE marking under the IVD Directive 98/79/EC, FDA 510(k) exempt status, and ISO 13485:2016 quality management certification. The system is suitable for a wide range of clinical applications, including protein purification for diagnostic assays, nucleic acid isolation for molecular diagnostics, and small-molecule analysis for drug development. The ÄKTApilot supports compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines, with features such as audit trails, electronic signatures, and data integrity controls in UNICORN 7.0 software. The system is compatible with GE Healthcare’s extensive portfolio of prepacked columns, including HiPrep, Sepharose, and Capto, as well as third-party columns for maximum flexibility. The ÄKTApilot is designed for integration with laboratory information systems (LIMS) and electronic health records (EHR), enabling seamless data transfer and compliance with 21 CFR Part 11 and EU Annex 11 regulations. The system’s stainless steel flow path and chemically resistant PEEK components ensure compatibility with a wide range of buffers, including organic solvents, acids, and bases, making it suitable for diverse applications such as affinity chromatography, ion exchange, and size exclusion. The system’s compact benchtop design and low noise operation make it ideal for use in clinical laboratories with limited space. The ÄKTApilot has been validated for use in regulated environments, with comprehensive documentation including installation qualification (IQ), operational qualification (OQ), and performance qualification (PQ) protocols.