Pre-owned / Used 2011 ILLUMINA MiSeq DNA ANALYZERS for Sale

Hardware Summary

MiSeq benchtop sequencer with 2x300bp read length, 15Gb output, 28-hour runtime, integrated fluidics, precise optical detection, and robust thermal control for high-fidelity sequencing.

Software Summary

MiSeq Control Software v4.0 with real-time base calling, integrated primary/secondary analysis, Illumina DRAGEN Bio-IT Platform compatibility, and FDA 21 CFR Part 11 compliant data manage...

Accessories Summary

Compatible with MiSeq Reagent Kits v3/v2, MiSeq Flow Cells, TruSeq DNA/RNA Library Prep Kits, Illumina BaseSpace cloud integration, and third-party bioinformatics tools like CLC Genomics ...

Hardware

The Illumina MiSeq is a compact, benchtop next-generation sequencing (NGS) system designed for high-throughput DNA analysis in clinical and research laboratories. The system features a modular architecture with integrated fluidics, optics, and thermal control systems, enabling precise temperature cycling and fluorescence detection. The MiSeq utilizes a 4-color, charge-coupled device (CCD) camera for high-resolution imaging of sequencing reactions, with a maximum output of 15 gigabases (Gb) per run and read lengths up to 2x300 base pairs (bp). The instrument includes a dedicated computer system with a touchscreen interface for real-time monitoring and control. Key hardware components include a solid-state laser excitation source, high-sensitivity photomultiplier tubes (PMTs) for signal detection, and a microfluidic flow cell for sample processing. The system supports automated cluster generation and sequencing by synthesis (SBS) chemistry, with a typical run time of approximately 28 ...

Software / Upgrade / OS / Applications / Worklist

The MiSeq is powered by MiSeq Control Software (MCS) v4.0, a comprehensive, user-friendly interface designed for seamless operation and data management. MCS provides real-time monitoring of sequencing runs, including base calling, quality score generation, and cluster identification. The software supports automated primary analysis, including demultiplexing and FASTQ file generation, with optional secondary analysis pipelines for variant calling and alignment. The MiSeq is compatible with Illumina's DRAGEN Bio-IT Platform, which offers accelerated secondary analysis with advanced algorithms for variant detection, copy number variation (CNV) analysis, and structural variant calling. The system complies with FDA 21 CFR Part 11 regulations for electronic records and signatures, ensuring data integrity and traceability. MiSeq Control Software integrates with Illumina BaseSpace, a cloud-based platform for data storage, sharing, and advanced bioinformatics analysis.

Accessories

The Illumina MiSeq ecosystem includes a range of high-quality accessories and consumables designed to optimize sequencing workflows and ensure consistent performance. Key accessories include MiSeq Reagent Kits (v2 and v3), which provide optimized chemistry for different read lengths and output requirements, with typical yields of 10-15 Gb per run. The MiSeq Flow Cell, a critical component for sequencing reactions, is available in standard and high-output configurations to accommodate varying sample throughput. Illumina offers a suite of library preparation kits, including TruSeq DNA and RNA Library Prep Kits, for efficient and reproducible sample processing. The MiSeq is compatible with Illumina's TruSight panels for targeted sequencing applications, such as oncology, inherited disease, and infectious disease detection. For data management and analysis, the MiSeq supports integration with Illumina BaseSpace, which provides cloud-based storage, sharing, and advanced bioinformatics to...

Warranty Inclusions & Exclusions

This pre-owned Illumina MiSeq is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The warranty includes a 12-month parts and labor coverage, with options for extended warranty plans up to 36 months, ensuring extended protection against unexpected failures. The warranty covers all critical components, including the optical detection system, fluidics module, thermal control unit, and integrated computer system. In the event of a malfunction, our technical support team provides rapid response and on-site service, with a guaranteed response time of 48 hours for critical issues. The warranty also includes access to Illumina's global service network, with certified engineers trained in MiSeq maintenance and repair. Additionally, the warranty covers software updates and technical support for the MiSeq Control Software and BaseSpace platform, ensuring compliance with the latest regulatory standards and performance optimizations.

Service History

This pre-owned Illumina MiSeq has undergone rigorous service and maintenance to ensure optimal performance and reliability. The instrument has been serviced annually by certified Illumina technicians, with detailed records of all maintenance activities, including fluidics system cleaning, optical calibration, thermal control validation, and software updates. The service history includes replacement of consumable components, such as flow cells, reagent lines, and PMTs, as per Illumina's recommended maintenance schedule. The instrument has been calibrated using Illumina's proprietary calibration kits to ensure accurate base calling and signal detection. Additionally, the service records include performance validation tests, such as sequencing runs with control samples, to confirm consistent output and data quality. The MiSeq has been used in a controlled laboratory environment with stable temperature and humidity conditions, minimizing the risk of environmental-related issues.

Packing List

The Illumina MiSeq is delivered with a complete set of components and accessories to ensure immediate readiness for sequencing workflows. The packing list includes the MiSeq instrument itself, mounted on a sturdy, mobile cart with integrated power supply and cable management. The cart is equipped with locking casters for stability and easy movement within the laboratory. The instrument is accompanied by a dedicated computer system with a touchscreen interface, preloaded with MiSeq Control Software v4.0 and Illumina BaseSpace integration. The packing list also includes a MiSeq Flow Cell, preloaded with sequencing reagents for the first run, along with a MiSeq Reagent Kit v3 (or v2, depending on configuration) for 2x300bp paired-end sequencing. Additional accessories include a set of calibration kits for optical and thermal validation, a maintenance kit with cleaning supplies and replacement consumables, and a service toolkit for routine upkeep.

Additional Information

This pre-owned Illumina MiSeq is a fully functional, high-performance next-generation sequencing system with a proven track record in clinical and research laboratories. The MiSeq complies with all relevant regulatory standards, including FDA 21 CFR Part 820 (Quality System Regulation), FDA 21 CFR Part 11 (Electronic Records and Signatures), and ISO 13485 (Medical Devices – Quality Management Systems). The instrument is CE-marked for use in the European Union, ensuring compliance with the In Vitro Diagnostic Directive (IVDD) 98/79/EC and the Medical Device Regulation (MDR) 2017/746. The MiSeq is also compliant with the Clinical Laboratory Improvement Amendments (CLIA) in the United States, making it suitable for use in certified clinical laboratories. The instrument has been validated for a wide range of clinical applications, including targeted gene panel sequencing for oncology, inherited disease testing, and infectious disease identification. The MiSeq supports workflows for microbial identification, metagenomic analysis, and microbiome profiling, with applications in antimicrobial resistance detection and outbreak tracking. The system is compatible with Illumina's TruSight panels, which are designed for specific clinical indications, such as the TruSight Oncology 500 for comprehensive cancer profiling. The MiSeq also supports custom amplicon sequencing for research applications, including gene expression analysis, epigenetic studies, and CRISPR screening. The instrument integrates seamlessly with hospital information systems (HIS) and laboratory information management systems (LIMS) via HL7 or FHIR standards, enabling automated data transfer and reporting. The MiSeq is equipped with robust cybersecurity features, including encrypted data storage, user authentication, and audit trails, ensuring compliance with HIPAA and GDPR regulations for patient data protection.