Pre-owned / Used 2011 ILLUMINA MiSeq DNA SEQUENCERS for Sale

Hardware Summary

Illumina MiSeq benchtop sequencer with 2x300bp read length, 15Gb output, 8-lane flow cell, 28-hour run time, 90% accuracy, USB/ETH/LAN connectivity, 100-240V power, 50-60Hz frequency, 150...

Software Summary

MiSeq Control Software v4.0+, Real-Time Analysis v2.6+, BaseSpace Suite, integrated primary/secondary analysis, automated cluster generation, error rate tracking, demultiplexing, FASTQ ge...

Accessories Summary

Compatible with MiSeq Reagent Kit v3, MiSeq Flow Cell, MiSeq PR2 Bottle, MiSeq Buffer Cartridge, MiSeq Wash Buffer, MiSeq Denaturation Solution, MiSeq Hybridization Buffer, MiSeq Tris-HCl...

Hardware

The Illumina MiSeq is a compact, benchtop next-generation sequencing (NGS) system designed for targeted sequencing, amplicon sequencing, and small whole-genome sequencing applications. The system features a 2x300bp paired-end read capability with a maximum output of 15 gigabases (Gb) per run, achieving up to 90% accuracy across all read lengths. The integrated 8-lane flow cell system supports multiplexing of up to 96 samples per run, with a rapid turnaround time of approximately 28 hours for a full run cycle. The instrument is equipped with a high-performance optical system featuring a 1.3 megapixel CMOS camera and a 4-color laser excitation system (488nm, 532nm, 640nm, 750nm) for precise fluorescence detection. The MiSeq operates on a 100-240V AC power supply with a frequency range of 50-60Hz and consumes 150W of power. Physical dimensions are 43.2cm (depth) x 30.5cm (width) x 43.2cm (height) with a net weight of 15.

Software / Upgrade / OS / Applications / Worklist

The MiSeq system operates on a dedicated Linux-based embedded platform running MiSeq Control Software (MCS) v4.0 or higher, which provides comprehensive instrument control, real-time monitoring, and automated sequencing workflow management. The software integrates seamlessly with Illumina's Real-Time Analysis (RTA) v2.6+ for primary data processing, including base calling, quality scoring (Phred Q scores), and cluster identification with an accuracy threshold of Q30 for >80% of bases. Secondary analysis is performed through the BaseSpace Suite, which offers automated demultiplexing, FASTQ file generation, and alignment to reference genomes using Burrows-Wheeler Aligner (BWA) or other supported algorithms. The system supports both single-end and paired-end sequencing workflows with customizable read lengths from 50bp to 300bp per read. Key software features include automatic error rate tracking, adapter trimming, quality filtering (minimum Q score threshold configurable), and real-ti...

Accessories

The Illumina MiSeq system requires a comprehensive set of consumables and accessories for optimal operation. Primary consumables include the MiSeq Reagent Kit v3 (2x300bp format) with 500-cycle output capacity, MiSeq Flow Cell (8-lane format) for sample loading, and MiSeq PR2 Bottle for sequencing reagent delivery. Additional critical consumables include the MiSeq Buffer Cartridge (containing hybridization buffer, wash buffer, and storage buffer), MiSeq Denaturation Solution (0.2N NaOH for template denaturation), MiSeq Hybridization Buffer (for probe-based applications), and MiSeq Tris-HCl/TE Buffer solutions for pH stabilization. The system utilizes specialized cleaning and maintenance reagents such as MiSeq Cleaning Solution for flow cell decontamination and MiSeq Storage Buffer for long-term instrument preservation. For sample preparation workflows, compatible accessories include the MiSeq Sample Purification Beads, MiSeq Resuspension Buffer, and MiSeq Elution Buffer.

Warranty Inclusions & Exclusions

This pre-owned Illumina MiSeq system is offered with comprehensive warranty and support options to ensure clinical-grade reliability and performance. The seller provides a minimum 90-day limited warranty covering all major components, including the optical system, flow cell stage, temperature control module, and embedded computer system. Extended warranty options are available for purchase, including a 1-year parts and labor warranty with 24/7 technical support, or a 2-year comprehensive warranty covering both hardware and software components. The warranty includes on-site service for critical failures, remote diagnostics via Illumina's ServiceLink platform, and priority access to certified field service engineers. Software support includes access to Illumina's BaseSpace Suite with 24/7 cloud-based troubleshooting, firmware updates, and compatibility with the latest MiSeq Control Software versions.

Service History

This Illumina MiSeq system has undergone rigorous preventive maintenance and service documentation throughout its operational lifespan, with all records available for review. The service history includes 12 documented preventive maintenance (PM) visits conducted at 6-month intervals by Illumina-certified field service engineers, with each visit including full system calibration, optical alignment verification, temperature uniformity testing, and flow cell stage precision measurement. Critical components such as the CMOS camera, laser excitation system, and cluster generation module have been replaced proactively based on performance degradation trends, with all replacements documented in the service log. The system has undergone two full performance validations against Illumina's factory specifications, including sequencing accuracy verification using the PhiX control library (Q30 >80% for all runs), cluster density optimization (target range 800-1200 clusters/mm²), and phasing/pre-...

Packing List

The complete packing and shipping configuration for this Illumina MiSeq system includes the main instrument unit with integrated computer and touchscreen display, a dedicated power cable (IEC 60320 C13 to C14), a USB 2.0 data cable for peripheral connections, the original operator's manual in English (with additional language inserts where applicable), the installation and service documentation CD-ROM, a set of calibration verification tools (including alignment target and QA/QC standards), a comprehensive accessory kit containing spare fuses, cleaning swabs, lint-free wipes, and maintenance reagents, and the original shipping crate with custom foam inserts for secure transport. The system is shipped with the flow cell stage in a locked position and all optical components protected by dust covers. The packing configuration meets ISTA 3A standards for international shipping, with additional cushioning for sensitive components such as the CMOS camera and laser assembly.

Additional Information

This pre-owned Illumina MiSeq system represents a cost-effective solution for clinical and research laboratories seeking high-throughput next-generation sequencing capabilities without the capital expenditure of new equipment. The system is ideally suited for clinical applications including targeted gene panel sequencing for oncology diagnostics, microbial identification and antimicrobial resistance profiling, noninvasive prenatal testing (NIPT), and pharmacogenomic analysis. The MiSeq's 2x300bp read length capability enables comprehensive coverage of clinically relevant genomic regions, with demonstrated performance in detecting single nucleotide polymorphisms (SNPs) at >99.9% accuracy and small insertions/deletions (indels) at >98% sensitivity. The system's compact benchtop design makes it suitable for installation in standard clinical laboratories with limited floor space, while its low power consumption (150W) and minimal heat dissipation (passive cooling with active temperature control) reduce facility requirements. Regulatory compliance includes FDA 510(k) clearance for in vitro diagnostic use (K163726) under the Clinical Laboratory Improvement Amendments (CLIA) of 1988, with demonstrated performance in proficiency testing programs such as the College of American Pathologists (CAP) NGS sequencing surveys. The system carries the CE IVD mark for European market distribution under EU In Vitro Diagnostic Regulation (IVDR) 2017/746, with documented compliance to EN ISO 13485:2016 and EN ISO 14971:2019 standards. Additional certifications include IEC 62304:2006 for medical device software lifecycle processes, IEC 60601-1-2:2014 for electromagnetic compatibility, and ISO 13485:2016 for quality management systems. The system has been validated for use with clinically relevant reference materials including the NIST Genome in a Bottle (GIAB) benchmark datasets and the Horizon Discovery SeraCare Seraseq NGS reference standards. Integration capabilities include HL7 v2.