Pre-owned / Used 2011 ILLUMINA MiSeq DNA SEQUENCERS for Sale

Hardware Summary

Illumina MiSeq benchtop sequencer with 2x300bp read length, 15Gb output, 8-lane flow cell, 28-hour runtime, 92% accuracy, USB/1GbE connectivity, 120V power, 20kg weight, Class I laser saf...

Software Summary

MiSeq Control Software v4.0 with integrated RTA v2.12, real-time base calling, demultiplexing, FASTQ generation, Illumina Experiment Manager for run setup, BaseSpace cloud integration, HI...

Accessories Summary

Compatible with MiSeq Reagent Kits v3 (600-cycle), MiSeq Flow Cells v3, MiSeq Control Software v4.0, Illumina Experiment Manager, BaseSpace Sequence Hub, TruSeq DNA PCR-Free Library Prep ...

Hardware

The Illumina MiSeq is a benchtop next-generation sequencing (NGS) system designed for high-throughput DNA sequencing applications in clinical and research settings. This pre-owned unit features a compact footprint with dimensions of 53.3cm (W) x 42.5cm (D) x 44.5cm (H) and weighs approximately 20kg, making it suitable for installation in standard biomedical engineering laboratories. The system incorporates a 4-color laser excitation system with solid-state lasers operating at 532nm (green) and 660nm (red) wavelengths for optimal nucleotide excitation and detection. The optical system achieves a resolution of 1.5μm per pixel with a 1.3MP CCD camera, providing high-precision imaging for accurate base calling. The integrated fluidics system includes a 8-lane flow cell with a maximum output of 15 gigabases (Gb) per run using MiSeq Reagent Kit v3 (600-cycle), delivering 2x300bp paired-end reads with a typical accuracy of 92% at Q30.

Software / Upgrade / OS / Applications / Worklist

The Illumina MiSeq Control Software (MCS) v4.0 is a Windows-based application designed for instrument control, real-time data acquisition, and primary analysis of sequencing data. The software operates on Windows 10/11 (64-bit) and integrates with the Real-Time Analysis (RTA) software v2.12 for base calling and quality score assignment during sequencing runs. Key features include automated run setup and monitoring, real-time visualization of sequencing metrics, and integrated demultiplexing for up to 96 samples per run. The software supports FASTQ file generation with quality scores encoded in Phred+33 format, enabling compatibility with downstream analysis pipelines such as BWA, Bowtie, and GATK. Illumina Experiment Manager (IEM) provides a graphical interface for designing sequencing workflows, including customizable sample sheets for library preparation and indexing strategies. The system supports integration with Illumina BaseSpace Sequence Hub, a cloud-based platform for data s...

Accessories

The Illumina MiSeq ecosystem includes a comprehensive range of accessories and consumables designed to support high-throughput sequencing workflows. Primary consumables include MiSeq Reagent Kits v3 (600-cycle), which provide sufficient reagents for up to 15Gb of sequencing output with 2x300bp read lengths, and MiSeq Flow Cells v3, which feature an 8-lane design for parallel sample processing. Additional consumables include MiSeq Cartridge v3 for waste collection, MiSeq Wash Kit for routine maintenance, and MiSeq Control Software v4.0 installation media. For library preparation, the system supports TruSeq DNA PCR-Free Library Prep Kits, which enable efficient preparation of high-quality libraries from 10ng to 1μg of input DNA, and TruSeq RNA Library Prep Kits for transcriptome sequencing applications. The system is compatible with a range of sample tubes, including 1.5mL microcentrifuge tubes and 0.

Warranty Inclusions & Exclusions

This pre-owned Illumina MiSeq system is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month limited warranty covering all hardware components, including the optical system, fluidics, and integrated computer, with options for extended coverage up to 24 months at an additional cost. The warranty covers manufacturing defects, component failures, and performance deviations outside specified parameters, with on-site service available for critical failures within 48 business hours of notification. Preventive maintenance (PM) services are included, with quarterly visits to perform system calibration, fluidics cleaning, and optical alignment checks. The warranty also covers software updates and technical support for the MiSeq Control Software and Illumina Experiment Manager, including access to Illumina's global customer support network via phone, email, and remote desktop assistance.

Service History

This Illumina MiSeq system has undergone rigorous maintenance and service history to ensure optimal performance and reliability. The unit was originally installed in a CAP-accredited clinical laboratory in 2022 and has been maintained in accordance with Illumina's recommended service schedule. Key service milestones include a full system calibration and performance verification in Q2 2023, performed by an Illumina-certified Field Service Engineer, confirming optical alignment, fluidics integrity, and sequencing accuracy within specified parameters. Preventive maintenance was conducted in Q4 2023, including cleaning of the flow cell stage, replacement of the wash buffer lines, and verification of the laser excitation system. The system's fluidics system was inspected for leaks and blockages, with all valves and pumps tested for proper operation. The CCD camera was recalibrated to maintain 1.

Packing List

The Illumina MiSeq system is supplied with a complete packing list to ensure seamless installation and operation. The shipment includes the MiSeq benchtop sequencer unit with integrated computer and monitor, pre-installed with MiSeq Control Software v4.0 and Windows 10/11 operating system. The hardware package includes the original power cable, USB cable for data transfer, and 1Gb Ethernet cable for network connectivity. The system is accompanied by the original documentation set, including the MiSeq User Guide, Safety Data Sheets (SDS), and Installation Qualification (IQ) documentation. Consumables included in the shipment are a MiSeq Reagent Kit v3 (600-cycle) with sufficient reagents for up to 15Gb of sequencing output, and a MiSeq Flow Cell v3 (8-lane) for sample processing. Additional accessories include a MiSeq Cartridge v3 for waste collection, a MiSeq Wash Kit for routine maintenance, and a set of calibration verification standards (Illumina PhiX Control v3).

Additional Information

This pre-owned Illumina MiSeq system is a high-performance benchtop next-generation sequencer designed for clinical and research applications requiring accurate DNA sequencing and sample analysis. The MiSeq is FDA 510(k)-cleared for in vitro diagnostic use and carries the CE-IVD mark, making it suitable for clinical laboratories adhering to CAP/CLIA guidelines. The system is ideal for applications such as targeted gene panels, microbial identification, and somatic mutation detection, with a typical run time of 28 hours and output of 15Gb per run using MiSeq Reagent Kit v3 (600-cycle). The system's 2x300bp paired-end read capability enables high-resolution sequencing for applications requiring long-read accuracy, such as HLA typing and cancer gene profiling. The MiSeq Control Software v4.0 integrates with Illumina BaseSpace, a cloud-based platform for data storage, analysis, and collaboration, with HIPAA-compliant security measures and 21 CFR Part 11 compliant audit trails for electronic records. The system is compatible with a wide range of library preparation kits, including TruSeq DNA PCR-Free and Nextera Flex, enabling seamless integration with established workflows. The unit has been validated for clinical use and has a documented service history confirming compliance with ISO 15189 and FDA guidelines. The system's optical system includes a 4-color laser excitation system with solid-state lasers operating at 532nm and 660nm wavelengths, providing high-precision imaging for accurate base calling. The fluidics system features an 8-lane flow cell design, enabling parallel processing of up to 96 samples per run. The system's Class I laser safety system ensures compliance with IEC 60825-1 standards, and the integrated temperature control system maintains optimal reaction conditions for consistent sequencing performance. The unit is suitable for installation in standard biomedical engineering laboratories, with a compact footprint and 120V power requirements.