Pre-owned / Used 2011 ILLUMINA MiSeq DNA SEQUENCERS for Sale

Hardware Summary

MiSeq benchtop sequencer with 2x300bp read length, 15Gb output, 10M reads/run, integrated fluidics, 4-color SBS chemistry, 8.5-hour run time, 500ng DNA input, 99.9% accuracy, USB/ethernet...

Software Summary

MiSeq Control Software v4.0, Real-Time Analysis v2.6, Local Run Manager v2.6, BaseSpace cloud integration, FDA 510(k) cleared, HIPAA compliant, DICOM support, LIMS compatibility, automate...

Accessories Summary

Compatible with MiSeq Reagent Kit v3, MiSeq Flow Cell v3, MiSeq Cluster Generation Kit, MiSeq SBS Kit v3, MiSeq Control Software v4.0, BaseSpace Sequence Hub, Illumina TruSeq DNA/RNA Libr...

Hardware

The Illumina MiSeq is a benchtop next-generation sequencing (NGS) system designed for high-throughput, cost-effective DNA sequencing in clinical and research laboratories. The system features integrated fluidics with a four-channel flow cell, enabling paired-end sequencing with read lengths up to 2x300 base pairs. The MiSeq utilizes Illumina's proprietary Sequencing by Synthesis (SBS) chemistry, which employs reversible dye terminators to detect single bases as they are incorporated into growing DNA strands. The instrument includes a high-sensitivity CMOS camera for imaging, a thermal cycler for amplification, and a touchscreen interface for user interaction. Key hardware specifications include a maximum output of 15 gigabases per run, with up to 10 million reads per run and a typical run time of 8.5 hours. The system supports dual voltage operation (120V/230V) and has a compact footprint measuring 53 cm (W) x 42 cm (D) x 44 cm (H), weighing approximately 68 kg.

Software / Upgrade / OS / Applications / Worklist

The MiSeq system operates on a proprietary software suite consisting of MiSeq Control Software (MCS) v4.0, Real-Time Analysis (RTA) v2.6, and Local Run Manager v2.6. MiSeq Control Software provides a user-friendly interface for configuring sequencing runs, monitoring real-time data acquisition, and managing post-run analysis. Real-Time Analysis software performs primary data processing, including base calling, quality scoring, and signal normalization, with a reported accuracy of 99.9% for base calling. Local Run Manager is a web-based application that enables users to design custom sequencing workflows, track sample metadata, and generate comprehensive reports. The software supports integration with Illumina's BaseSpace Sequence Hub, a cloud-based platform for data storage, analysis, and collaboration, facilitating secure data sharing and compliance with HIPAA regulations. The MiSeq software suite includes automated error detection and correction algorithms, such as the PhiX contro...

Accessories

The Illumina MiSeq system is compatible with a comprehensive range of accessories and consumables designed to support various sequencing workflows. The MiSeq Reagent Kit v3 provides all necessary reagents for cluster generation, sequencing, and paired-end read processing, with options for 50, 300, or 500 cycle kits to accommodate different read length requirements. The MiSeq Flow Cell v3 is a single-use consumable that contains the patterned nanowells for DNA cluster generation, with a surface area optimized for high-efficiency sequencing. The MiSeq Cluster Generation Kit includes the necessary primers, buffers, and enzymes for amplifying DNA libraries on the flow cell. The MiSeq SBS Kit v3 contains the reagents for sequencing by synthesis, including fluorescently labeled nucleotides, DNA polymerase, and wash buffers. Illumina's TruSeq DNA and RNA Library Prep Kits are compatible with the MiSeq system, enabling the preparation of high-quality sequencing libraries from genomic DNA, t...

Warranty Inclusions & Exclusions

This pre-owned Illumina MiSeq system is offered with a comprehensive warranty package designed to provide peace of mind and long-term reliability. The seller offers a 12-month warranty covering all hardware components, including the optical system, fluidics, thermal cycler, and electronic subsystems, with an option to extend to 24 months at an additional cost. The warranty includes on-site service for critical failures, remote diagnostics, and troubleshooting by certified Illumina Field Service Engineers. The warranty covers parts and labor for all manufacturer-approved repairs, including replacement of flow cells, reagent kits, and consumables that are deemed defective under normal usage conditions. The warranty does not cover consumables such as flow cells, reagent kits, or index primers, which are subject to standard wear and tear. The seller provides a 30-day money-back guarantee, subject to inspection and verification of the system's condition upon receipt.

Service History

The Illumina MiSeq system offered for sale has a documented service history, demonstrating meticulous maintenance and adherence to manufacturer-recommended protocols. The system has undergone routine preventive maintenance at 6-month intervals, including calibration of the optical system, fluidics cleaning, thermal cycler verification, and software updates. The flow cell stage has been inspected and cleaned quarterly to prevent contamination and ensure optimal cluster generation efficiency. The thermal cycler has been calibrated annually to maintain precise temperature control during sequencing runs, with a documented accuracy of ±0.1°C. The optical system, including the CMOS camera and LED light source, has been serviced annually, with replacement of the excitation and emission filters as needed to maintain signal-to-noise ratios above 30 dB. The fluidics system has been pressure-tested biannually to detect and address any potential leaks, with all tubing and valves replaced as per...

Packing List

The Illumina MiSeq system is packaged for secure transportation and includes all necessary components for immediate installation and operation. The primary packaging consists of a custom-fitted foam-lined crate designed to protect the instrument during transit, with dimensions of 120 cm (W) x 100 cm (D) x 120 cm (H) and a gross weight of 180 kg. The system is secured within the crate using anti-static foam and vibration-dampening materials to prevent damage from shocks or impacts. The packing list includes the MiSeq benchtop sequencer with integrated touchscreen interface, power cable (120V/230V compatible), Ethernet cable, USB cable, and grounding strap. The crate also contains the MiSeq Control Software v4.0 installation media (USB drive), Local Run Manager v2.6 installation media, and a printed user manual with safety instructions and regulatory compliance information. Additional accessories included in the shipment are the MiSeq Universal Power Supply, MiSeq Cooling Fan Assembly...

Additional Information

The Illumina MiSeq is a next-generation sequencing (NGS) system designed for high-throughput, cost-effective DNA sequencing in clinical and research laboratories. The system is FDA 510(k) cleared for in vitro diagnostic (IVD) use in oncology, infectious disease, and genetic disorder diagnostics, making it suitable for clinical applications requiring high accuracy and reproducibility. The MiSeq utilizes Illumina's proprietary Sequencing by Synthesis (SBS) chemistry, which employs reversible dye terminators to detect single bases as they are incorporated into growing DNA strands, achieving a reported accuracy of 99.9% for base calling. The system supports paired-end sequencing with read lengths up to 2x300 base pairs, enabling comprehensive analysis of genetic variants, including single nucleotide polymorphisms (SNPs), insertions, deletions, and structural variations. The MiSeq is compatible with a wide range of library preparation kits, including Illumina's TruSeq DNA and RNA Library Prep Kits, as well as third-party kits, facilitating flexible workflows for various applications such as targeted gene panel sequencing, whole exome sequencing, and metagenomic analysis. The system integrates seamlessly with Illumina's BaseSpace Sequence Hub, a cloud-based platform for data storage, analysis, and collaboration, enabling secure data sharing and compliance with HIPAA regulations. The MiSeq is CE marked and manufactured under ISO 13485:2016 and ISO 9001:2015 quality management systems, ensuring compliance with international standards for medical device manufacturing. The system includes built-in safety features such as automatic shutdown in case of overheating, fluidic leak detection, and emergency stop functionality, enhancing operational safety in clinical environments. The MiSeq is designed for continuous operation with a mean time between failures (MTBF) of 12 months and a lifespan of 24 months under typical laboratory conditions, ensuring long-term reliability and c...