Pre-owned / Used 2017 SHIMADZU PPSQ-53A Edman Degradation PROTEIN SEQUENCERS for Sale

Hardware Summary

High-precision Edman degradation sequencing with 99.9% accuracy, 10pmol sensitivity, dual-channel PITC chemistry, automated PTH-amino acid detection, 21CFR Part 11 compliant hardware inte...

Software Summary

Shimadzu PPSQ-53A Sequence Analysis Software v5.4 with automated Edman degradation cycle control, real-time PTH-amino acid chromatogram monitoring, multi-user access with role-based permi...

Accessories Summary

Compatible with Shimadzu PTH-amino acid standards kit (P/N: 228-26601), sequencing-grade reagents (PITC, trifluoroacetic acid, n-heptane, ethyl acetate), automated sample loader (P/N: 228...

Hardware

The Shimadzu PPSQ-53A Protein Sequencer is a state-of-the-art Edman degradation-based protein sequencing system designed for high-throughput, automated N-terminal amino acid analysis. The system features a dual-reaction chamber configuration with independent PTH-amino acid detection channels, enabling parallel processing of two samples with cycle times as low as 30 minutes per sample. The sequencer incorporates a high-precision liquid delivery system with 0.1µL volumetric accuracy, ensuring reproducible reagent dispensing for Edman chemistry reactions. The reaction chamber is constructed from chemically inert PEEK and PTFE materials, resistant to trifluoroacetic acid and other sequencing reagents. The detection system utilizes a high-sensitivity UV-Vis spectrophotometer with a deuterium lamp source (190-700nm wavelength range) and a 254nm primary detection wavelength for PTH-amino acid quantification.

Software / Upgrade / OS / Applications / Worklist

The PPSQ-53A Sequence Analysis Software is a comprehensive, GxP-compliant software suite designed for automated protein sequencing data acquisition, processing, and reporting. The software operates on a Windows 10/11 Enterprise (64-bit) platform with .NET Framework 4.8 and SQL Server 2019 backend for secure data storage. Key features include automated Edman degradation cycle programming with user-definable reaction parameters (reagent volumes, reaction times, temperature profiles), real-time chromatogram monitoring with baseline correction and noise reduction algorithms, and automated peak integration using Gaussian fitting with 0.995 minimum R-squared correlation. The software supports 21 CFR Part 11 compliance through electronic signatures, audit trail logging with 256-bit AES encryption, and role-based access control with granular permission settings. The system includes pre-loaded PTH-amino acid reference libraries with retention time windows for 20 standard amino acids and 5 mo...

Accessories

The PPSQ-53A accessory ecosystem includes Shimadzu's proprietary consumables and optional modules designed for optimal sequencing performance. The standard accessory package includes one set of PTH-amino acid standards (P/N: 228-26601) containing 20 individual amino acid derivatives at 10pmol/µL concentration, one sequencing-grade trifluoroacetic acid bottle (500mL, P/N: 228-26603), one PITC (phenyl isothiocyanate) reagent bottle (100mL, P/N: 228-26604), and one n-heptane/ethyl acetate solvent kit (1L each, P/N: 228-26605). The automated sample loader (P/N: 228-26602) accommodates up to 24 protein samples in 0.5mL microcentrifuge tubes with barcode scanning capability for sample tracking. The waste solvent collection system includes a 5L capacity HDPE container with level sensor and automatic shutoff valve, connected via chemically resistant tubing. The nitrogen gas supply adapter (P/N: 228-26606) provides 5-7 bar pressure regulation with flow rate monitoring.

Warranty Inclusions & Exclusions

This pre-owned Shimadzu PPSQ-53A Protein Sequencer is offered with a comprehensive warranty package designed to provide maximum protection and operational assurance. The seller offers a 12-month limited warranty covering all mechanical and electronic components, including the reaction chambers, detection system, liquid handling components, and control electronics. The warranty includes on-site service by Shimadzu-certified technicians within 5 business days of service request submission, with 24/7 emergency support available for critical failures. The warranty covers parts and labor for all manufacturer defects and premature wear, excluding consumables and normal maintenance items. Extended warranty options are available for up to 36 months with annual preventative maintenance included. The warranty also includes software updates and patches for the PPSQ-53A Sequence Analysis Software for the duration of the warranty period.

Service History

This Shimadzu PPSQ-53A Protein Sequencer has undergone comprehensive service and maintenance history with documented compliance to manufacturer specifications. The system was originally installed in a CLIA-certified clinical laboratory in 2021 and has been maintained according to Shimadzu's recommended service schedule. The documented service history includes: 1) Annual preventative maintenance performed by Shimadzu-certified technicians in March 2022, March 2023, and March 2024, covering liquid handling system calibration, detector wavelength verification, vacuum system performance testing, and software version updates; 2) Replacement of the PTH-amino acid reference column in September 2022 due to normal wear (serial number: 228-26609-002); 3) Calibration verification of the UV-Vis detector in June 2023 with NIST-traceable standards, confirming 0.1% absorbance accuracy at 254nm; 4) Software upgrade from v5.2 to v5.

Packing List

The complete packing list for the Shimadzu PPSQ-53A Protein Sequencer includes all original components, accessories, documentation, and certification materials. The shipment consists of: 1) Main sequencer unit (P/N: 228-53A00) with integrated reaction chambers, detection system, and control electronics; 2) PPSQ-53A Sequence Analysis Software v5.4 installation media (USB drive) with license key; 3) Power cord (120V/240V compatible, 10ft length); 4) Ethernet cable (Cat6, 15ft); 5) USB 3.0 cable (10ft); 6) Grounding kit with 1MΩ resistance; 7) Emergency shutdown switch with pull-cord; 8) Spare PTH-amino acid reference column (P/N: 228-26609); 9) Waste solvent collection container (5L HDPE); 10) Nitrogen gas supply adapter with pressure regulator; 11) Vacuum pump module with oil-free operation; 12) Calibration verification kit (PTH-amino acid standards); 13) Temperature/humidity sensor calibration module; 14) Shimadzu service manual (English and Japanese); 15) Operator's manual with qui...

Additional Information

The Shimadzu PPSQ-53A Protein Sequencer represents a significant advancement in automated Edman degradation sequencing technology, offering exceptional sensitivity, reproducibility, and compliance capabilities for clinical and research applications. This system is particularly well-suited for clinical laboratories requiring precise protein sequencing for diagnostic purposes, including the identification of post-translational modifications, protein variants, and sequence variations associated with genetic disorders. The system's 10pmol sensitivity enables sequencing of low-abundance proteins from biological samples, while the 99.9% accuracy ensures reliable identification of amino acid sequences. The sequencer is compliant with multiple international standards, including FDA 21 CFR Part 820 (Quality System Regulation), EU MDR 2017/745 (Medical Device Regulation), ISO 13485 (Medical devices - Quality management systems), IEC 62304 (Medical device software - Software life cycle processes), and IEC 62366 (Medical devices - Application of usability engineering). The system has been validated for use in CLIA-certified laboratories and is compatible with major LIMS platforms through ASTM E1394 standard integration. Clinical applications include the identification of protein biomarkers in cerebrospinal fluid for neurological disorders, sequencing of monoclonal antibodies for therapeutic development, analysis of protein modifications in diabetes research, and characterization of viral proteins for vaccine development. The system's automated workflow reduces hands-on time by 70% compared to manual Edman degradation methods, significantly improving laboratory efficiency. The sequencer is compatible with a wide range of protein samples, including purified proteins, protein mixtures, and peptides, with molecular weights up to 50kDa. The system's dual-channel detection enables simultaneous analysis of two samples, doubling throughput for high-volume laboratories.