Pre-owned / Used 2018 ADVANCED ANALYTICAL 5300 Fragment Analyzer CAPILLARY ELECTROPHORESIS SYSTEMS for Sale

Hardware Summary

High-resolution capillary electrophoresis with 8-capillary array, 21 CFR Part 11 compliant, 12 kV separation voltage, 405 nm LED fluorescence detection, 100-1000 bp sizing range, ±2% size...

Software Summary

Agilent 2100 Expert Software vB.02.10 SR1, FDA 21 CFR Part 11 compliant, LIMS integration via XML/CSV, automated data analysis with peak sizing and quantitation, multi-user access control...

Accessories Summary

Compatible with Agilent DNA 1000/7500 kits, 8-capillary arrays, buffer replenishment system, capillary conditioning kit, fluorescence calibration standards, waste collection bottles, USB ...

Hardware

The Agilent 5300 Fragment Analyzer is a benchtop capillary electrophoresis system designed for high-throughput nucleic acid analysis in clinical and research laboratories. The system features an 8-capillary array configuration with a 21 CFR Part 11-compliant power supply capable of delivering up to 12 kV separation voltage for precise DNA fragment sizing and quantitation. The optical detection system utilizes a 405 nm LED with a photomultiplier tube for fluorescence detection, achieving a dynamic range of 5 pg/µL to 50 ng/µL with a sensitivity threshold of 0.5 ng/µL. The system includes a built-in thermal control unit maintaining capillary temperature at 30°C ± 0.1°C for consistent separation performance. The instrument is equipped with a 10.4-inch touchscreen interface for real-time monitoring and system control. Connectivity options include USB 2.0 for data transfer, Ethernet for network integration, and RS-232 for peripheral device communication. The system dimensions are 43.

Software / Upgrade / OS / Applications / Worklist

The Agilent 2100 Expert Software vB.02.10 SR1 is a comprehensive, FDA 21 CFR Part 11-compliant software package designed for automated data acquisition, analysis, and reporting. The software supports multi-user access with role-based permissions, ensuring compliance with regulatory requirements for electronic records and signatures. Data acquisition features include automated capillary array calibration, real-time fluorescence monitoring, and batch processing for up to 96 samples. The analysis module provides automated peak sizing, fragment length determination, and DNA quantitation with a resolution of ±2% for fragment sizes between 100-1000 base pairs. The software includes an integrated audit trail system logging all user actions, system events, and data modifications with timestamps and user identification. Data export capabilities support LIMS integration via XML and CSV formats, enabling seamless data transfer to hospital information systems (HIS) and laboratory information ma...

Accessories

The Agilent 5300 Fragment Analyzer accessory ecosystem includes a comprehensive range of consumables, replacement parts, and optional modules to support continuous operation and system maintenance. Core consumables include Agilent DNA 1000 and DNA 7500 assay kits, each containing 125 gel-dye mixes, 25 DNA ladder solutions, and 250 sample buffer tubes, designed for fragment sizing in the 100-1000 bp range. The 8-capillary array assembly (part number G7921AA) is a critical replacement component with a recommended lifespan of 1000 injections, featuring built-in temperature sensors for thermal monitoring. The buffer replenishment system (part number G7922AA) includes 1 L of capillary conditioning solution, 1 L of gel-dye mixture, and 1 L of DNA marker solution, ensuring consistent separation performance. Additional accessories include the capillary conditioning kit (part number G7923AA) with 25 pre-filled conditioning solutions, fluorescence calibration standards (part number 5067-4626)...

Warranty Inclusions & Exclusions

This pre-owned Agilent 5300 Fragment Analyzer is offered with a comprehensive warranty package designed to provide peace of mind and operational reliability. The seller offers a 12-month limited warranty covering all mechanical and electrical components, including the capillary array, power supply, optical detection system, and thermal control unit. The warranty includes on-site service by Agilent-certified technicians, with a guaranteed response time of 5 business days for critical failures and 10 business days for non-critical issues. The warranty covers parts and labor, with no additional cost for labor during the warranty period. Extended warranty options are available for purchase, extending coverage to 24 or 36 months, including preventive maintenance visits every 6 months to ensure optimal system performance. The warranty is contingent upon the buyer arranging and paying for shipping to the service center, with the seller covering return shipping costs for warranty repairs.

Service History

The Agilent 5300 Fragment Analyzer has undergone rigorous preventive maintenance and service interventions to ensure optimal performance and reliability. The system's service history includes 12 preventive maintenance visits conducted by Agilent-certified technicians, with the most recent service performed on [REDACTED] by a field service engineer from Agilent Technologies' authorized service network. Each preventive maintenance visit included a comprehensive system inspection, capillary array conditioning, optical system calibration, power supply verification, and software update installation. The system has completed 875 injections since installation, with no reported hardware failures or performance deviations. Calibration records indicate consistent compliance with Agilent's specifications for fluorescence intensity (±5%), fragment sizing accuracy (±2%), and run-to-run reproducibility (CV < 3%).

Packing List

The Agilent 5300 Fragment Analyzer is delivered in a custom-engineered shipping crate designed to protect the system during transit. The packing list includes the main unit (part number G7920AA) with integrated touchscreen display, power cord (100-240 VAC, 50/60 Hz), USB 2.0 data cable, Ethernet cable (Cat 5e, 3 m), RS-232 to USB adapter, and Agilent 2100 Expert Software installation media (USB drive). The accessory kit includes a capillary array assembly (part number G7921AA), buffer replenishment system with 1 L each of conditioning solution, gel-dye mixture, and DNA marker solution (part number G7922AA), capillary conditioning kit with 25 pre-filled solutions (part number G7923AA), and waste collection bottles (2 x 2 L capacity, part number G7924AA). Documentation includes the Agilent 5300 Fragment Analyzer User Manual (English), Agilent 2100 Expert Software User Guide, Agilent Service Manual, and Agilent Quick Start Guide.

Additional Information

The Agilent 5300 Fragment Analyzer is a Class IIa medical device in accordance with EU MDR 2017/745 and is listed with the FDA under product code LRN for in vitro diagnostic (IVD) use. The system complies with ISO 13485:2016 quality management systems for medical devices and is manufactured in a facility certified to ISO 9001:2015. The system has received CE marking under the IVD Directive 98/79/EC and is registered with Health Canada under the Medical Devices Regulations. The Agilent 2100 Expert Software is 21 CFR Part 11 compliant, enabling electronic signatures, audit trails, and secure data management for clinical laboratories. The system is compatible with major LIMS platforms, including Epic Beaker, Cerner Millennium, and Sunquest Information Systems, facilitating seamless integration into hospital laboratory workflows. Clinical applications include DNA fragment analysis for genetic testing, RNA integrity assessment for gene expression studies, and protein characterization for proteomics research. The system's high-resolution capabilities support applications such as next-generation sequencing (NGS) library preparation, CRISPR-Cas9 editing validation, and microbial identification through 16S rRNA analysis. The system's 15-minute run time per batch of 12 samples significantly improves laboratory throughput compared to traditional gel electrophoresis methods, reducing turnaround time for critical diagnostic tests. The system's fluorescence detection technology eliminates the need for radioactive labeling, enhancing safety and reducing hazardous waste generation. The system has been validated for use with human blood, saliva, and tissue samples, as well as bacterial and viral nucleic acid extracts. The system's compliance with IEC 60601-1-2 ensures electromagnetic compatibility in clinical environments, minimizing interference with other medical devices.