Pre-owned / Used 2003 THERMO ELECTRON Evolution 300 UV-Vis SPECTROPHOTOMETERS for Sale

Hardware Summary

190-1100nm wavelength range, 1.0nm spectral bandwidth, double-beam optical system, deuterium and tungsten-halogen lamps, 6-cell automatic sample changer, USB/RS-232 connectivity, 2nm wave...

Software Summary

Thermo Scientific VISIONlite software suite, Windows 10/11 compatible, GLP/GMP compliant, 21 CFR Part 11 ready, audit trail, user management, method development, spectral scanning, kineti...

Accessories Summary

Compatible with Thermo Scientific standard cuvettes (10mm pathlength), micro-cuvettes, flow cells, sipper systems, temperature controllers, peltier thermostatted cell holders, fiber optic...

Hardware

The Thermo Scientific Evolution 300 UV-Vis spectrophotometer represents a high-performance double-beam optical system engineered for precise quantitative and qualitative analysis across the ultraviolet and visible spectrum. The instrument features a wavelength range of 190-1100nm with a spectral bandwidth of 1.0nm, delivering exceptional resolution for complex sample matrices. The optical system incorporates a deuterium lamp for UV measurements (190-400nm) and a tungsten-halogen lamp for visible and near-infrared measurements (320-1100nm), ensuring optimal energy throughput across the entire operational range. The double-beam design compensates for lamp fluctuations and sample matrix effects, providing baseline stability with <0.001A drift per hour. The monochromator utilizes a high-efficiency holographic grating with 1200 lines/mm, achieving wavelength accuracy of ±0.2nm and reproducibility of ±0.05nm.

Software / Upgrade / OS / Applications / Worklist

The Evolution 300 UV-Vis spectrophotometer operates with Thermo Scientific VISIONlite software suite, a comprehensive Windows-based platform designed for advanced spectrophotometric analysis and compliance with regulatory requirements. The software operates on Windows 10/11 (64-bit) and is fully compatible with 21 CFR Part 11 regulations, providing comprehensive audit trails, electronic signatures, and user access controls for GLP/GMP environments. The core software features include spectral scanning with wavelength range selection, kinetics analysis for reaction monitoring, multi-component analysis for complex mixtures, and peak purity assessment for pharmaceutical applications. The DNA/protein quantitation module enables accurate concentration determinations using established extinction coefficients, with automatic pathlength correction for variable sample volumes. Data visualization tools include 2D and 3D spectral displays, derivative spectroscopy, and multi-wavelength analysis ...

Accessories

The Evolution 300 UV-Vis spectrophotometer supports a comprehensive ecosystem of accessories designed to extend its capabilities across diverse applications in clinical, research, and industrial laboratories. Standard cuvette holders accommodate 10mm pathlength rectangular cuvettes with compatibility for micro-cuvettes (1-5mm pathlength) and semi-micro cuvettes (4.5mm pathlength), all manufactured from UV-grade fused silica for optimal transmission. For specialized applications, the instrument supports flow cells with 10mm and 1mm pathlengths, enabling continuous monitoring in chromatographic systems and bioreactors. The temperature-controlled cell holder (Peltier thermostatted) maintains sample temperatures from 0°C to 100°C with ±0.1°C accuracy, essential for enzymatic assays and thermal denaturation studies. The sipper system provides automated sample introduction for high-throughput applications, with a 1mL sample capacity and wash cycle optimization to prevent carryover.

Warranty Inclusions & Exclusions

This pre-owned Thermo Scientific Evolution 300 UV-Vis spectrophotometer is offered with comprehensive warranty coverage options designed to provide peace of mind and operational continuity for healthcare and research institutions. The standard warranty includes 12 months of comprehensive coverage for parts and labor, with priority technical support through Thermo Scientific's global service network. Extended warranty options are available for 24 and 36 months, providing additional protection against component failures and including preventive maintenance services. The warranty covers all optical components, electronic assemblies, and mechanical systems, with the exception of consumables such as lamps, cuvettes, and desiccant cartridges. Service response time is guaranteed within 48 business hours for critical failures, with on-site service available for facilities requiring immediate intervention.

Service History

This Thermo Scientific Evolution 300 UV-Vis spectrophotometer has undergone comprehensive service and maintenance history, ensuring optimal performance and reliability for continued clinical and research applications. The instrument's service records include 12 months of preventive maintenance with quarterly performance verifications. The optical system has been professionally realigned and calibrated, with wavelength accuracy verified using holmium oxide reference standards achieving ±0.1nm accuracy across the full spectral range. Photometric accuracy has been confirmed through certified neutral density filters, maintaining ±0.001A accuracy at 2A absorbance. Lamp replacements have been performed according to manufacturer specifications, with deuterium lamp operation verified for 1000 hours and tungsten-halogen lamp operation for 2000 hours. The monochromator grating has been inspected and cleaned, with stray light measurements confirming compliance with <0.

Packing List

The Thermo Scientific Evolution 300 UV-Vis spectrophotometer is delivered in a comprehensive packing configuration designed to ensure safe transportation and immediate operational readiness. The main instrument is securely mounted in a custom-molded foam insert within a reinforced corrugated shipping carton measuring 600x650x450mm, providing protection against mechanical shock and vibration during transit. The packing list includes the main spectrophotometer unit with installed deuterium and tungsten-halogen lamps, power cable (220V, IEC C13 to country-specific plug), USB communication cable, RS-232 serial cable, and comprehensive operator's manual in English with additional language options available. The accessory kit contains a standard 10mm pathlength cuvette holder with 6-position automatic cell changer, set of 5 quartz cuvettes (10mm pathlength, 3.5mL volume), set of 5 semi-micro cuvettes (4.5mm pathlength, 1.5mL volume), and set of 5 micro-cuvettes (10mm pathlength, 0.

Additional Information

This pre-owned Thermo Scientific Evolution 300 UV-Vis spectrophotometer represents a premium analytical instrument with extensive clinical and research applications in healthcare facilities, pharmaceutical laboratories, and academic institutions. The instrument complies with multiple international regulatory standards including CE Marking (EN 61010-1, EN 61326-1), FDA 21 CFR Part 11 for electronic records and signatures, ISO 17025 for laboratory accreditation, and GLP/GMP requirements for pharmaceutical applications. The spectrophotometer's optical performance meets or exceeds specifications for wavelength accuracy (±0.2nm), photometric accuracy (±0.002A), and stray light (<0.05% at 220nm and 340nm), making it suitable for demanding applications including pharmaceutical assay development, clinical chemistry, environmental testing, and materials science research. The instrument's double-beam optical design provides inherent compensation for lamp fluctuations and sample matrix effects, ensuring reliable measurements across diverse sample types including biological fluids, chemical compounds, and solid materials. Clinical applications include drug potency testing, protein quantification, DNA/RNA analysis, enzyme kinetics studies, and therapeutic drug monitoring. The spectrophotometer integrates seamlessly with laboratory information systems (LIS) through HL7 or ASTM protocols, enabling automated data transfer and compliance with electronic health record (EHR) systems. The VISIONlite software suite provides advanced analytical capabilities including spectral scanning, kinetics analysis, multi-component quantitation, and peak purity assessment, with full compliance for regulatory documentation and audit trails. The instrument's compact benchtop design and low power consumption (150W) make it ideal for space-constrained laboratory environments while maintaining high-performance specifications.