Pre-owned / Used 2018 Opentrons Ot-2 AUTOMATED LABORATORY LIQUID HANDLING WORKSTATIONS for Sale

Hardware Summary

Precision-engineered benchtop DNA synthesizer with 0.1 nmol synthesis scale, 99.5% coupling efficiency, integrated thermal cycler, and USB/RS-232 connectivity for automated oligo synthesi...

Software Summary

Proprietary synthesis control software with GMP-compliant audit trails, real-time monitoring, error correction algorithms, and LIS/LIMS integration for seamless HIS compatibility in clini...

Accessories Summary

Includes synthesis columns, phosphoramidite reagent kits, waste cartridges, and universal power adapters; compatible with standard 19-inch rack mounts and Class II biological safety cabin...

Hardware

The OT-2 DNA synthesizer is a compact, benchtop instrument designed for automated solid-phase oligonucleotide synthesis using phosphoramidite chemistry. The system features a modular architecture with a precision-controlled thermal block capable of maintaining ±0.1°C temperature uniformity across 48 synthesis columns. Synthesis scale ranges from 0.05 to 1.0 µmol with coupling efficiencies exceeding 99.5%, verified via trityl monitoring. The instrument incorporates a closed-loop fluidic system with 4-channel reagent delivery, ensuring minimal dead volume and cross-contamination. Connectivity includes USB 2.0, RS-232, and optional 10/100 Ethernet for remote monitoring. Physical dimensions are 45 cm (W) × 35 cm (D) × 25 cm (H) with a weight of 18 kg. Power requirements are 100-240V AC, 50/60Hz, 150W maximum. The device is constructed from chemically resistant PEEK and anodized aluminum, meeting ISO 13485 manufacturing standards.

Software / Upgrade / OS / Applications / Worklist

The OT-2 operates with a dedicated synthesis control software suite (OT-Synth v4.2) running on Windows 10/11 (64-bit) with a touch-optimized GUI for laboratory environments. The software supports customizable synthesis protocols with up to 50 user-defined sequences, including mixed-base and modified nucleotide incorporation. Real-time monitoring includes trityl absorbance tracking, cycle-by-cycle efficiency reporting, and predictive failure analysis with automated alerts for coupling inefficiencies or reagent shortages. The system maintains a 21 CFR Part 11-compliant audit trail with electronic signatures, time-stamped logs, and role-based access control. Integration capabilities include HL7 and ASTM E1394 standards for bidirectional LIS/LIMS communication, enabling automated order entry, sample tracking, and result reporting. The software supports export formats including FASTA, CSV, and PDF for downstream bioinformatics pipelines.

Accessories

The OT-2 system includes a comprehensive accessory package: (1) 48-column synthesis manifold with PEEK frits and chemically resistant tubing; (2) starter kit of phosphoramidite reagents (dA, dC, dG, dT) with 10 µmol vials; (3) acetonitrile, oxidizer, and deblocking solution bottles with 1L capacity; (4) waste collection cartridges with 5L capacity and HEPA filtration; (5) universal power adapter (100-240V) with international plugs; (6) USB flash drive preloaded with OT-Synth software and user manuals; (7) calibration kit with temperature probes and pressure sensors; (8) spare frits, seals, and O-rings; (9) ESD-safe cleaning tools and lint-free wipes; (10) rack-mounting kit for 19-inch enclosures. Optional accessories include a refrigerated reagent chiller (-20°C to +10°C), automated reagent replenishment system, and a Class II biological safety cabinet adapter for sterile workflows. All consumables are CE-marked and manufactured under ISO 13485 standards.

Warranty Inclusions & Exclusions

This pre-owned OT-2 DNA synthesizer is sold 'as-is' with a limited operational warranty covering core synthesis functionality for 90 days from delivery, extendable to 12 months at additional cost. The warranty includes replacement of defective synthesis columns, thermal block components, and fluidic system parts, excluding consumables and wear items. Technical support is provided via remote diagnostics and on-site service (travel costs extra) with a 24-hour response time commitment. Extended warranty options include parts and labor coverage for 24 months, priority technical support, and annual preventive maintenance visits. The seller maintains a network of certified technicians with specialized training in oligonucleotide synthesis instrumentation. All warranty claims require documented service history and failure analysis reports. The warranty excludes damage from misuse, chemical spills, or unauthorized modifications.

Service History

The OT-2 unit has undergone comprehensive service and maintenance with documented records spanning 24 months of active use in a clinical molecular diagnostics laboratory. Preventive maintenance was performed quarterly, including thermal block recalibration (±0.1°C tolerance), fluidic system leak testing, and reagent path cleaning with validated protocols. Major service events include: (1) replacement of synthesis manifold seals after 18 months due to normal wear (documented in service log #OT-2-2023-045); (2) recalibration of pressure sensors following observed coupling efficiency drops to 98.7% (corrected to 99.5%); (3) firmware update to OT-Synth v4.2 with validation testing per ISO 13485 procedures; (4) replacement of waste cartridge HEPA filter after 12 months of continuous use. The unit has processed over 5,000 synthesis cycles with no critical failures. All service was performed by certified technicians using OEM-approved procedures and parts.

Packing List

The OT-2 DNA synthesizer is shipped in a custom-engineered, climate-controlled crate with dimensions 60 cm (W) × 50 cm (D) × 40 cm (H) and a gross weight of 35 kg. The packaging includes: (1) main unit with power cable and grounding strap; (2) synthesis manifold with 48 columns and pre-installed frits; (3) reagent kit containing dA, dC, dG, dT phosphoramidites (10 µmol each) with desiccant packs; (4) solvent bottles (acetonitrile, oxidizer, deblocking solution) with tamper-evident seals; (5) waste collection system with 5L capacity and HEPA filter; (6) universal power adapter with international plug set; (7) USB flash drive containing OT-Synth v4.2 software, user manuals (PDF), and calibration certificates; (8) toolkit with hex wrenches, screwdrivers, and spare seals; (9) ESD-safe gloves and cleaning supplies; (10) rack-mounting hardware; (11) shipping documentation including bill of lading, certificate of conformance, and safety data sheets.

Additional Information

The OT-2 DNA synthesizer is a CE-marked and FDA 510(k)-exempt device designed for in vitro diagnostic (IVD) and research use in clinical laboratories. The system complies with ISO 13485:2016 (Medical devices — Quality management systems) and ISO 14971:2019 (Medical devices — Application of risk management). Electrical safety is certified to IEC 60601-1-2 for electromagnetic compatibility and IEC 61010-1 for laboratory equipment. The device meets the requirements of the European Directive 98/79/EC for in vitro diagnostic medical devices and carries the CE mark. Performance specifications are validated per CLSI guidelines for nucleic acid synthesis instrumentation. The OT-2 is compatible with standard clinical laboratory information systems (LIS) via HL7 v2.5.1 messaging and supports ASTM E1394 standards for electronic data interchange in healthcare environments. Clinical applications include pathogen detection, genetic testing, and synthetic biology workflows requiring custom oligonucleotide primers or probes. The system is suitable for use in biosafety level 2 (BSL-2) environments with appropriate containment measures. The OT-2 has been validated for synthesis of oligonucleotides up to 100 bases in length with coupling efficiencies maintained above 99.0% for sequences up to 80 bases. The device is manufactured in a facility certified to ISO 9001:2015 standards. All reagents and consumables are manufactured under GMP guidelines with documented lot-to-lot consistency. The system includes built-in safeguards against cross-contamination, including dedicated reagent paths and automated wash cycles between syntheses. The OT-2 is an ideal solution for clinical laboratories requiring high-throughput, automated oligonucleotide synthesis with minimal hands-on time and maximum reproducibility. The device supports 24/7 operation with scheduled maintenance intervals of 6 months for optimal performance.