Pre-owned / Used 2016 BECTON DICKINSON LSRFortessa X-20 FLOW CYTOMETERS for Sale

Hardware Summary

LSR II platform with 20-parameter detection, 4-laser configuration (405nm, 488nm, 561nm, 640nm), high-performance optical system, advanced fluidics with 96-well plate compatibility, 150,0...

Software Summary

BD FACSDiva v9.0 software with multi-user licensing, real-time data acquisition and analysis, automated compensation with spillover matrix calculation, customizable experiment templates, ...

Accessories Summary

Compatible with BD FACSFlow sheath fluid, BD TruCount tubes for absolute counting, BD Calibrite beads for daily QC, BD CS&T research beads for performance tracking, 96-well and 384-well p...

Hardware

The BD LSRFortessa X-20 is a high-performance benchtop flow cytometer designed for multi-parameter cellular analysis in research and clinical laboratories. The system features a 4-laser optical configuration (405nm violet, 488nm blue, 561nm yellow-green, 640nm red) with 20 fluorescence detectors, enabling simultaneous detection of up to 18 colors plus forward and side scatter parameters. The optical bench incorporates high-efficiency dichroic mirrors and bandpass filters optimized for minimal spectral overlap, achieving <2% coefficient of variation (CV) in fluorescence resolution. The fluidics system includes a high-precision syringe pump with 96-well plate compatibility and automated sample loader, supporting both tube and plate-based workflows. The instrument maintains a 5-decade dynamic range with a maximum acquisition rate of 150,000 events per second, ensuring rapid data collection for rare event detection.

Software / Upgrade / OS / Applications / Worklist

The BD LSRFortessa X-20 operates with BD FACSDiva software v9.0, a comprehensive acquisition and analysis platform designed for multi-user environments in clinical and research settings. The software supports real-time data acquisition with customizable experiment templates, automated compensation calculation using spillover matrices, and advanced gating strategies for complex multi-parameter analysis. The platform includes integrated modules for cell cycle analysis, proliferation tracking, and apoptosis detection using Annexin V and caspase assays. Data export capabilities include FCS 3.1 format for compatibility with third-party analysis software, as well as direct PDF, CSV, and PowerPoint output for report generation. The software is fully compliant with FDA 21 CFR Part 11 requirements for electronic records and signatures, including secure user authentication, audit trails, and data integrity controls.

Accessories

The BD LSRFortessa X-20 system is supported by a comprehensive ecosystem of certified accessories and consumables designed for optimal performance and regulatory compliance. Key accessories include BD FACSFlow sheath fluid (CE marked, ISO 15189 compliant) for maintaining consistent fluid dynamics, BD TruCount absolute counting tubes for precise cell enumeration, and BD Calibrite beads for daily quality control verification. The system supports both 96-well and 384-well microplate formats for high-throughput applications, with automated plate loaders available as optional upgrades. Temperature-controlled tube holders maintain sample integrity during extended acquisitions, while the sample agitation system prevents cell settling in prolonged experiments. HEPA-filtered enclosure options provide biosafety level 2 (BSL-2) containment for handling potentially biohazardous samples. The system is compatible with BD's full range of fluorescence-labeled antibodies and reagents, including CE-m...

Warranty Inclusions & Exclusions

This pre-owned BD LSRFortessa X-20 flow cytometer is offered with comprehensive warranty and support options designed to meet the needs of clinical and research laboratories. The seller provides a 12-month limited warranty covering all mechanical and optical components, including the laser systems, fluidics, and detection electronics. The warranty includes on-site service by certified BD engineers with response time within 5 business days for critical failures. Extended warranty options are available at additional cost, covering parts and labor for up to 36 months. The warranty excludes consumables, wear-and-tear items, and damage resulting from improper operation or unauthorized modifications. The seller offers a 30-day money-back guarantee with full refund if the instrument fails initial performance verification tests. Technical support is provided via dedicated hotline with 24/7 availability for emergency troubleshooting.

Service History

This BD LSRFortessa X-20 flow cytometer has undergone comprehensive service and maintenance with complete documentation available. The instrument has been serviced annually by certified BD engineers in accordance with the manufacturer's preventive maintenance schedule, including laser alignment verification, fluidics system calibration, and optical bench cleaning. All laser systems (405nm, 488nm, 561nm, 640nm) have been performance-tested with <2% CV in fluorescence resolution, meeting BD's specifications for optimal detection sensitivity. The fluidics system has been calibrated for consistent sample acquisition rates and minimal carryover between samples, with verification using BD's performance verification beads. The optical filters and dichroic mirrors have been inspected and cleaned, with no degradation in transmission efficiency observed. The system has undergone electrical safety testing in accordance with IEC 60601-1 standards, with all components passing insulation resistan...

Packing List

The BD LSRFortessa X-20 flow cytometer is delivered in a custom-fitted shipping crate with dimensions 120cm x 100cm x 180cm and weight approximately 180kg. The packaging includes heavy-duty foam inserts with vibration dampening for optical component protection, as well as desiccant packs to prevent moisture damage during transit. The shipment includes the main instrument with installed 4-laser optical bench, high-performance computer workstation with pre-installed BD FACSDiva software, 24-inch medical-grade monitor with protective screen cover, USB keyboard and mouse, Ethernet cable for network connectivity, power cable with international plug adapters, and comprehensive user documentation including operation manual, service manual, and quick reference guide. Additional components include the automated sample loader with barcode scanner, temperature-controlled sample chamber, HEPA filtration system (if installed), and all necessary mounting hardware for bench installation.

Additional Information

This BD LSRFortessa X-20 flow cytometer represents a premium-grade analytical instrument with extensive clinical and research applications in oncology, immunology, and cell biology. The system is manufactured by Becton Dickinson, a global leader in flow cytometry technology with over 50 years of innovation in cellular analysis. The LSRFortessa X-20 platform is FDA-cleared for in vitro diagnostic use in the United States and carries CE IVD marking for European markets, meeting all applicable regulatory requirements including EU MDR 2017/746 and FDA 21 CFR Part 820. The instrument is compliant with ISO 15189 standards for medical laboratories, ISO 13485 for quality management systems, and IEC 60601-1 for electrical safety. Clinical applications include hematological malignancy characterization, minimal residual disease detection, stem cell enumeration, immune profiling in cancer immunotherapy, and vaccine development research. The system's 20-parameter detection capability enables comprehensive immunophenotyping of complex cell populations, including rare event analysis with high sensitivity and specificity. The instrument features automated compensation with real-time spillover matrix calculation, eliminating manual compensation errors and ensuring reproducible results across operators. The system supports integration with laboratory information systems (LIS) via HL7 protocols, enabling automated result reporting and compliance with electronic health record (EHR) requirements. The BD FACSDiva software includes advanced analysis modules for cell cycle analysis, proliferation tracking, and apoptosis detection, with export capabilities compatible with FlowJo, FCS Express, and other third-party analysis platforms. The instrument's high acquisition rate (150,000 events/sec) and 5-decade dynamic range make it suitable for both high-throughput screening and rare event detection applications.